Chicken bone marrow mesenchymal stem cells improve lung and distal organ injury

Abstract Mesenchymal stem cells (MSCs) are associated with pulmonary protection and longevity. We separated chicken bone marrow-derived mesenchymal stem cells (BM-MSCs); investigated whether BM-MSCs can improve lipopolysaccharide (LPS)-induced lung and distal organ injury; and explored the underlyin...

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Autores principales:	Hexuan Niu, Hanan Song, Yuhan Guan, Xianchun Zong, Ruili Niu, Shiyu Zhao, Cong Liu, Wenzaixiang Yan, Weijun Guan, Xishuai Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/303e1f513b55468b93788ff94df0b7ea
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spelling	oai:doaj.org-article:303e1f513b55468b93788ff94df0b7ea2021-12-02T17:19:16ZChicken bone marrow mesenchymal stem cells improve lung and distal organ injury10.1038/s41598-021-97383-42045-2322https://doaj.org/article/303e1f513b55468b93788ff94df0b7ea2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97383-4https://doaj.org/toc/2045-2322Abstract Mesenchymal stem cells (MSCs) are associated with pulmonary protection and longevity. We separated chicken bone marrow-derived mesenchymal stem cells (BM-MSCs); investigated whether BM-MSCs can improve lipopolysaccharide (LPS)-induced lung and distal organ injury; and explored the underlying mechanisms. Ninety-six male ICR (6 weeks old) mice were randomly divided into three groups: Sham, LPS, and LPS + MSC groups. The mice were intratracheally injected with 5 mg/kg LPS to induce acute lung injury (ALI). The histopathological severity of injury to the lung, liver, kidney, heart, and aortic tissues was detected. Wet/dry ratio, protein concentrations in bronchoalveolar lavage fluid (BALF), BALF cell counts, inflammatory cytokine levels in serum, inflammatory cytokine gene expression, and oxidative stress-related indicators were detected. In addition, a survival analysis was performed in sixty male ICR mice (6 weeks old, 18–20 g). This study used chicken BM-MSCs, which are easier to obtain and more convenient than other animal or human MSCs, and have MSC-associated properties, such as a colony forming ability, multilineage differentiation potential, and certain phenotypes. BM-MSCs administration significantly improved the survival rate, systemic inflammation, and the histopathological severity of lung, liver, kidney, and aortic injury during ALI. BM-MSCs administration reduced the levels of inflammatory factors in BALF, the infiltration of neutrophils, and oxidative stress injury in lung tissue. In addition, BM-MSCs administration reduced TRL4 and Mdy88 mRNA expression during ALI. Chicken BM-MSCs serve as a potential alternative resource for stem cell therapy and exert a prominent effect on LPS-induced ALI and extrapulmonary injury, in part through TRL4/Mdy88 signaling and inhibition of neutrophil inflammation and oxidative stress injury.Hexuan NiuHanan SongYuhan GuanXianchun ZongRuili NiuShiyu ZhaoCong LiuWenzaixiang YanWeijun GuanXishuai WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hexuan Niu Hanan Song Yuhan Guan Xianchun Zong Ruili Niu Shiyu Zhao Cong Liu Wenzaixiang Yan Weijun Guan Xishuai Wang Chicken bone marrow mesenchymal stem cells improve lung and distal organ injury
description	Abstract Mesenchymal stem cells (MSCs) are associated with pulmonary protection and longevity. We separated chicken bone marrow-derived mesenchymal stem cells (BM-MSCs); investigated whether BM-MSCs can improve lipopolysaccharide (LPS)-induced lung and distal organ injury; and explored the underlying mechanisms. Ninety-six male ICR (6 weeks old) mice were randomly divided into three groups: Sham, LPS, and LPS + MSC groups. The mice were intratracheally injected with 5 mg/kg LPS to induce acute lung injury (ALI). The histopathological severity of injury to the lung, liver, kidney, heart, and aortic tissues was detected. Wet/dry ratio, protein concentrations in bronchoalveolar lavage fluid (BALF), BALF cell counts, inflammatory cytokine levels in serum, inflammatory cytokine gene expression, and oxidative stress-related indicators were detected. In addition, a survival analysis was performed in sixty male ICR mice (6 weeks old, 18–20 g). This study used chicken BM-MSCs, which are easier to obtain and more convenient than other animal or human MSCs, and have MSC-associated properties, such as a colony forming ability, multilineage differentiation potential, and certain phenotypes. BM-MSCs administration significantly improved the survival rate, systemic inflammation, and the histopathological severity of lung, liver, kidney, and aortic injury during ALI. BM-MSCs administration reduced the levels of inflammatory factors in BALF, the infiltration of neutrophils, and oxidative stress injury in lung tissue. In addition, BM-MSCs administration reduced TRL4 and Mdy88 mRNA expression during ALI. Chicken BM-MSCs serve as a potential alternative resource for stem cell therapy and exert a prominent effect on LPS-induced ALI and extrapulmonary injury, in part through TRL4/Mdy88 signaling and inhibition of neutrophil inflammation and oxidative stress injury.
format	article
author	Hexuan Niu Hanan Song Yuhan Guan Xianchun Zong Ruili Niu Shiyu Zhao Cong Liu Wenzaixiang Yan Weijun Guan Xishuai Wang
author_facet	Hexuan Niu Hanan Song Yuhan Guan Xianchun Zong Ruili Niu Shiyu Zhao Cong Liu Wenzaixiang Yan Weijun Guan Xishuai Wang
author_sort	Hexuan Niu
title	Chicken bone marrow mesenchymal stem cells improve lung and distal organ injury
title_short	Chicken bone marrow mesenchymal stem cells improve lung and distal organ injury
title_full	Chicken bone marrow mesenchymal stem cells improve lung and distal organ injury
title_fullStr	Chicken bone marrow mesenchymal stem cells improve lung and distal organ injury
title_full_unstemmed	Chicken bone marrow mesenchymal stem cells improve lung and distal organ injury
title_sort	chicken bone marrow mesenchymal stem cells improve lung and distal organ injury
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/303e1f513b55468b93788ff94df0b7ea
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_version_	1718381018151387136

Chicken bone marrow mesenchymal stem cells improve lung and distal organ injury

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