Urinary peptidomics reveals proteases involved in idiopathic membranous nephropathy

Abstract Background Idiopathic membranous nephropathy (IMN) is a cause of nephrotic syndrome that is increasing in incidence but has unclear pathogenesis. Urinary peptidomics is a promising technology for elucidating molecular mechanisms underlying diseases. Dysregulation of the proteolytic system i...

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Autores principales:	Baoxu Lin, Jianhua Liu, Yue Zhang, Yabin Wu, Shixiao Chen, Yibo Bai, Qiuying Liu, Xiaosong Qin
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Urinary peptidomics Idiopathic membranous nephropathy Proteases Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/304b1ce3c3e740239e3d34ff25cd62ed
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spelling	oai:doaj.org-article:304b1ce3c3e740239e3d34ff25cd62ed2021-11-28T12:23:10ZUrinary peptidomics reveals proteases involved in idiopathic membranous nephropathy10.1186/s12864-021-08155-31471-2164https://doaj.org/article/304b1ce3c3e740239e3d34ff25cd62ed2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12864-021-08155-3https://doaj.org/toc/1471-2164Abstract Background Idiopathic membranous nephropathy (IMN) is a cause of nephrotic syndrome that is increasing in incidence but has unclear pathogenesis. Urinary peptidomics is a promising technology for elucidating molecular mechanisms underlying diseases. Dysregulation of the proteolytic system is implicated in various diseases. Here, we aimed to conduct urinary peptidomics to identify IMN-related proteases. Results Peptide fingerprints indicated differences in naturally produced urinary peptide components among 20 healthy individuals, 22 patients with IMN, and 15 patients with other kidney diseases. In total, 1,080 peptide-matched proteins were identified, 279 proteins differentially expressed in the urine of IMN patients were screened, and 32 proteases were predicted; 55 of the matched proteins were also differentially expressed in the kidney tissues of IMN patients, and these were mainly involved in the regulation of proteasome-, lysosome-, and actin cytoskeleton-related signaling pathways. The 32 predicted proteases showed abnormal expression in the glomeruli of IMN patients based on Gene Expression Omnibus databases. Western blot revealed abnormal expression of calpain, matrix metalloproteinase 14, and cathepsin S in kidney tissues of patients with IMN. Conclusions This work shown the calpain/matrix metalloproteinase/cathepsin axis might be dysregulated in IMN. Our study is the first to systematically explore the role of proteases in IMN by urinary peptidomics, which are expected to facilitate discovery of better biomarkers for IMN.Baoxu LinJianhua LiuYue ZhangYabin WuShixiao ChenYibo BaiQiuying LiuXiaosong QinBMCarticleUrinary peptidomicsIdiopathic membranous nephropathyProteasesBiotechnologyTP248.13-248.65GeneticsQH426-470ENBMC Genomics, Vol 22, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Urinary peptidomics Idiopathic membranous nephropathy Proteases Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	Urinary peptidomics Idiopathic membranous nephropathy Proteases Biotechnology TP248.13-248.65 Genetics QH426-470 Baoxu Lin Jianhua Liu Yue Zhang Yabin Wu Shixiao Chen Yibo Bai Qiuying Liu Xiaosong Qin Urinary peptidomics reveals proteases involved in idiopathic membranous nephropathy
description	Abstract Background Idiopathic membranous nephropathy (IMN) is a cause of nephrotic syndrome that is increasing in incidence but has unclear pathogenesis. Urinary peptidomics is a promising technology for elucidating molecular mechanisms underlying diseases. Dysregulation of the proteolytic system is implicated in various diseases. Here, we aimed to conduct urinary peptidomics to identify IMN-related proteases. Results Peptide fingerprints indicated differences in naturally produced urinary peptide components among 20 healthy individuals, 22 patients with IMN, and 15 patients with other kidney diseases. In total, 1,080 peptide-matched proteins were identified, 279 proteins differentially expressed in the urine of IMN patients were screened, and 32 proteases were predicted; 55 of the matched proteins were also differentially expressed in the kidney tissues of IMN patients, and these were mainly involved in the regulation of proteasome-, lysosome-, and actin cytoskeleton-related signaling pathways. The 32 predicted proteases showed abnormal expression in the glomeruli of IMN patients based on Gene Expression Omnibus databases. Western blot revealed abnormal expression of calpain, matrix metalloproteinase 14, and cathepsin S in kidney tissues of patients with IMN. Conclusions This work shown the calpain/matrix metalloproteinase/cathepsin axis might be dysregulated in IMN. Our study is the first to systematically explore the role of proteases in IMN by urinary peptidomics, which are expected to facilitate discovery of better biomarkers for IMN.
format	article
author	Baoxu Lin Jianhua Liu Yue Zhang Yabin Wu Shixiao Chen Yibo Bai Qiuying Liu Xiaosong Qin
author_facet	Baoxu Lin Jianhua Liu Yue Zhang Yabin Wu Shixiao Chen Yibo Bai Qiuying Liu Xiaosong Qin
author_sort	Baoxu Lin
title	Urinary peptidomics reveals proteases involved in idiopathic membranous nephropathy
title_short	Urinary peptidomics reveals proteases involved in idiopathic membranous nephropathy
title_full	Urinary peptidomics reveals proteases involved in idiopathic membranous nephropathy
title_fullStr	Urinary peptidomics reveals proteases involved in idiopathic membranous nephropathy
title_full_unstemmed	Urinary peptidomics reveals proteases involved in idiopathic membranous nephropathy
title_sort	urinary peptidomics reveals proteases involved in idiopathic membranous nephropathy
publisher	BMC
publishDate	2021
url	https://doaj.org/article/304b1ce3c3e740239e3d34ff25cd62ed
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_version_	1718408004608458752

Urinary peptidomics reveals proteases involved in idiopathic membranous nephropathy

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