New Insights into Plant TPK Ion Channel Evolution

Potassium (K) is a crucial element of plant nutrition, involved in many physiological and molecular processes. K<sup>+</sup> membrane transporters are playing a pivotal role in K<sup>+</sup> transport and tissue distribution as well as in various plant stress responses and de...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Siarhei A. Dabravolski, Stanislav V. Isayenkov
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	KCO3 TPK two pore potassium channel molecular phylogeny molecular evolution Botany QK1-989
Acceso en línea:	https://doaj.org/article/304eb98eb1234f6992ad79b4bc9205a2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:304eb98eb1234f6992ad79b4bc9205a2
record_format	dspace
spelling	oai:doaj.org-article:304eb98eb1234f6992ad79b4bc9205a22021-11-25T18:45:22ZNew Insights into Plant TPK Ion Channel Evolution10.3390/plants101123282223-7747https://doaj.org/article/304eb98eb1234f6992ad79b4bc9205a22021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2328https://doaj.org/toc/2223-7747Potassium (K) is a crucial element of plant nutrition, involved in many physiological and molecular processes. K<sup>+</sup> membrane transporters are playing a pivotal role in K<sup>+</sup> transport and tissue distribution as well as in various plant stress responses and developmental processes. Two-pore K<sup>+</sup>-channels (TPKs) are essential to maintain plant K<sup>+</sup> homeostasis and are mainly involved in potassium transport from the vacuoles to the cytosol. Besides vacuolar specialization, some TPK members display different membrane localization including plasma membrane, protein storage vacuole membrane, and probably the organelles. In this manuscript, we elucidate the evolution of the voltage-independent TPK (two-pore K<sup>+</sup>-channels) family, which could be represented in some species by one pore, K<sup>+</sup>-inward rectifier (Kir)-like channels. A comprehensive investigation of existing databases and application of modern bioinformatic tools allowed us to make a detailed phylogenetic inventory of TPK/KCO3 (KCO: potassium channel, outward rectifying) channels through many taxa and gain insight into the evolutionary origin of TPK family proteins. Our results reveal the fundamental evolutional difference between the first and second pores, traced throughout multiple taxa variations in the ion selection filter motif, presence of thansposon, and methylation site in the proximity of some KCO members and suggest virus-mediated horizontal transfer of a KCO3-like ancestor by viruses. Additionally, we suggest several interconnected hypotheses to explain the obtained results and provide a theoretical background for future experimental validation.Siarhei A. DabravolskiStanislav V. IsayenkovMDPI AGarticleKCO3TPKtwo pore potassium channelmolecular phylogenymolecular evolutionBotanyQK1-989ENPlants, Vol 10, Iss 2328, p 2328 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	KCO3 TPK two pore potassium channel molecular phylogeny molecular evolution Botany QK1-989
spellingShingle	KCO3 TPK two pore potassium channel molecular phylogeny molecular evolution Botany QK1-989 Siarhei A. Dabravolski Stanislav V. Isayenkov New Insights into Plant TPK Ion Channel Evolution
description	Potassium (K) is a crucial element of plant nutrition, involved in many physiological and molecular processes. K<sup>+</sup> membrane transporters are playing a pivotal role in K<sup>+</sup> transport and tissue distribution as well as in various plant stress responses and developmental processes. Two-pore K<sup>+</sup>-channels (TPKs) are essential to maintain plant K<sup>+</sup> homeostasis and are mainly involved in potassium transport from the vacuoles to the cytosol. Besides vacuolar specialization, some TPK members display different membrane localization including plasma membrane, protein storage vacuole membrane, and probably the organelles. In this manuscript, we elucidate the evolution of the voltage-independent TPK (two-pore K<sup>+</sup>-channels) family, which could be represented in some species by one pore, K<sup>+</sup>-inward rectifier (Kir)-like channels. A comprehensive investigation of existing databases and application of modern bioinformatic tools allowed us to make a detailed phylogenetic inventory of TPK/KCO3 (KCO: potassium channel, outward rectifying) channels through many taxa and gain insight into the evolutionary origin of TPK family proteins. Our results reveal the fundamental evolutional difference between the first and second pores, traced throughout multiple taxa variations in the ion selection filter motif, presence of thansposon, and methylation site in the proximity of some KCO members and suggest virus-mediated horizontal transfer of a KCO3-like ancestor by viruses. Additionally, we suggest several interconnected hypotheses to explain the obtained results and provide a theoretical background for future experimental validation.
format	article
author	Siarhei A. Dabravolski Stanislav V. Isayenkov
author_facet	Siarhei A. Dabravolski Stanislav V. Isayenkov
author_sort	Siarhei A. Dabravolski
title	New Insights into Plant TPK Ion Channel Evolution
title_short	New Insights into Plant TPK Ion Channel Evolution
title_full	New Insights into Plant TPK Ion Channel Evolution
title_fullStr	New Insights into Plant TPK Ion Channel Evolution
title_full_unstemmed	New Insights into Plant TPK Ion Channel Evolution
title_sort	new insights into plant tpk ion channel evolution
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/304eb98eb1234f6992ad79b4bc9205a2
work_keys_str_mv	AT siarheiadabravolski newinsightsintoplanttpkionchannelevolution AT stanislavvisayenkov newinsightsintoplanttpkionchannelevolution
_version_	1718410702705655808

New Insights into Plant TPK Ion Channel Evolution

Ejemplares similares