Mitochondrial genomes of two parasitic Cuscuta species lack clear evidence of horizontal gene transfer and retain unusually fragmented ccmF C genes

Abstract Background The intimate association between parasitic plants and their hosts favours the exchange of genetic material, potentially leading to horizontal gene transfer (HGT) between plants. With the recent publication of several parasitic plant nuclear genomes, there has been considerable fo...

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Autores principales:	Benjamin M. Anderson, Kirsten Krause, Gitte Petersen
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Chloroplast Dodder Intracellular transfer Mitogenome Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/3059577038a44ac5b5d144442d596df9
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spelling	oai:doaj.org-article:3059577038a44ac5b5d144442d596df92021-11-14T12:26:43ZMitochondrial genomes of two parasitic Cuscuta species lack clear evidence of horizontal gene transfer and retain unusually fragmented ccmF C genes10.1186/s12864-021-08105-z1471-2164https://doaj.org/article/3059577038a44ac5b5d144442d596df92021-11-01T00:00:00Zhttps://doi.org/10.1186/s12864-021-08105-zhttps://doaj.org/toc/1471-2164Abstract Background The intimate association between parasitic plants and their hosts favours the exchange of genetic material, potentially leading to horizontal gene transfer (HGT) between plants. With the recent publication of several parasitic plant nuclear genomes, there has been considerable focus on such non-sexual exchange of genes. To enhance the picture on HGT events in a widely distributed parasitic genus, Cuscuta (dodders), we assembled and analyzed the organellar genomes of two recently sequenced species, C. australis and C. campestris, making this the first account of complete mitochondrial genomes (mitogenomes) for this genus. Results The mitogenomes are 265,696 and 275,898 bp in length and contain a typical set of mitochondrial genes, with 10 missing or pseudogenized genes often lost from angiosperm mitogenomes. Each mitogenome also possesses a structurally unusual ccmF C gene, which exhibits splitting of one exon and a shift to trans-splicing of its intron. Based on phylogenetic analysis of mitochondrial genes from across angiosperms and similarity-based searches, there is little to no indication of HGT into the Cuscuta mitogenomes. A few candidate regions for plastome-to-mitogenome transfer were identified, with one suggestive of possible HGT. Conclusions The lack of HGT is surprising given examples from the nuclear genomes, and may be due in part to the relatively small size of the Cuscuta mitogenomes, limiting the capacity to integrate foreign sequences.Benjamin M. AndersonKirsten KrauseGitte PetersenBMCarticleChloroplastDodderIntracellular transferMitogenomeBiotechnologyTP248.13-248.65GeneticsQH426-470ENBMC Genomics, Vol 22, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Chloroplast Dodder Intracellular transfer Mitogenome Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	Chloroplast Dodder Intracellular transfer Mitogenome Biotechnology TP248.13-248.65 Genetics QH426-470 Benjamin M. Anderson Kirsten Krause Gitte Petersen Mitochondrial genomes of two parasitic Cuscuta species lack clear evidence of horizontal gene transfer and retain unusually fragmented ccmF C genes
description	Abstract Background The intimate association between parasitic plants and their hosts favours the exchange of genetic material, potentially leading to horizontal gene transfer (HGT) between plants. With the recent publication of several parasitic plant nuclear genomes, there has been considerable focus on such non-sexual exchange of genes. To enhance the picture on HGT events in a widely distributed parasitic genus, Cuscuta (dodders), we assembled and analyzed the organellar genomes of two recently sequenced species, C. australis and C. campestris, making this the first account of complete mitochondrial genomes (mitogenomes) for this genus. Results The mitogenomes are 265,696 and 275,898 bp in length and contain a typical set of mitochondrial genes, with 10 missing or pseudogenized genes often lost from angiosperm mitogenomes. Each mitogenome also possesses a structurally unusual ccmF C gene, which exhibits splitting of one exon and a shift to trans-splicing of its intron. Based on phylogenetic analysis of mitochondrial genes from across angiosperms and similarity-based searches, there is little to no indication of HGT into the Cuscuta mitogenomes. A few candidate regions for plastome-to-mitogenome transfer were identified, with one suggestive of possible HGT. Conclusions The lack of HGT is surprising given examples from the nuclear genomes, and may be due in part to the relatively small size of the Cuscuta mitogenomes, limiting the capacity to integrate foreign sequences.
format	article
author	Benjamin M. Anderson Kirsten Krause Gitte Petersen
author_facet	Benjamin M. Anderson Kirsten Krause Gitte Petersen
author_sort	Benjamin M. Anderson
title	Mitochondrial genomes of two parasitic Cuscuta species lack clear evidence of horizontal gene transfer and retain unusually fragmented ccmF C genes
title_short	Mitochondrial genomes of two parasitic Cuscuta species lack clear evidence of horizontal gene transfer and retain unusually fragmented ccmF C genes
title_full	Mitochondrial genomes of two parasitic Cuscuta species lack clear evidence of horizontal gene transfer and retain unusually fragmented ccmF C genes
title_fullStr	Mitochondrial genomes of two parasitic Cuscuta species lack clear evidence of horizontal gene transfer and retain unusually fragmented ccmF C genes
title_full_unstemmed	Mitochondrial genomes of two parasitic Cuscuta species lack clear evidence of horizontal gene transfer and retain unusually fragmented ccmF C genes
title_sort	mitochondrial genomes of two parasitic cuscuta species lack clear evidence of horizontal gene transfer and retain unusually fragmented ccmf c genes
publisher	BMC
publishDate	2021
url	https://doaj.org/article/3059577038a44ac5b5d144442d596df9
work_keys_str_mv	AT benjaminmanderson mitochondrialgenomesoftwoparasiticcuscutaspecieslackclearevidenceofhorizontalgenetransferandretainunusuallyfragmentedccmfcgenes AT kirstenkrause mitochondrialgenomesoftwoparasiticcuscutaspecieslackclearevidenceofhorizontalgenetransferandretainunusuallyfragmentedccmfcgenes AT gittepetersen mitochondrialgenomesoftwoparasiticcuscutaspecieslackclearevidenceofhorizontalgenetransferandretainunusuallyfragmentedccmfcgenes
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Mitochondrial genomes of two parasitic Cuscuta species lack clear evidence of horizontal gene transfer and retain unusually fragmented ccmF C genes

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