Alterations of lipid metabolism provide serologic biomarkers for the detection of asymptomatic versus symptomatic COVID-19 patients

Abstract COVID-19 pandemic exerts a health care emergency around the world. The illness severity is heterogeneous. It is mostly unknown why some individuals who are positive for SARS-CoV-2 antibodies stay asymptomatic while others show moderate to severe disease symptoms. Reliable biomarkers for ear...

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Autores principales:	Alhaji H. Janneh, Mohamed Faisal Kassir, Connor J. Dwyer, Paramita Chakraborty, Jason S. Pierce, Patrick A. Flume, Hong Li, Satish N. Nadig, Shikhar Mehrotra, Besim Ogretmen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/305a4cbf085d4c1e8de8df3259f7cba4
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spelling	oai:doaj.org-article:305a4cbf085d4c1e8de8df3259f7cba42021-12-02T16:24:50ZAlterations of lipid metabolism provide serologic biomarkers for the detection of asymptomatic versus symptomatic COVID-19 patients10.1038/s41598-021-93857-72045-2322https://doaj.org/article/305a4cbf085d4c1e8de8df3259f7cba42021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93857-7https://doaj.org/toc/2045-2322Abstract COVID-19 pandemic exerts a health care emergency around the world. The illness severity is heterogeneous. It is mostly unknown why some individuals who are positive for SARS-CoV-2 antibodies stay asymptomatic while others show moderate to severe disease symptoms. Reliable biomarkers for early detection of the disease are urgently needed to attenuate the virus’s spread and help make early treatment decisions. Bioactive sphingolipids play a crucial role in the regulation of viral infections and pro-inflammatory responses involved in the severity of COVID-19. However, any roles of sphingolipids in COVID-19 development or detection remain unknown. In this study, lipidomics measurement of serum sphingolipids demonstrated that reduced sphingosine levels are highly associated with the development of symptomatic COVID-19 in the majority (99.24%) SARS-CoV-2-infected patients compared to asymptomatic counterparts. The majority of asymptomatic individuals (73%) exhibited increased acid ceramidase (AC) in their serum, measured by Western blotting, consistent with elevated sphingosine levels compared to SARS-CoV-2 antibody negative controls. AC protein was also reduced in almost all of the symptomatic patients’ serum, linked to reduced sphingosine levels, measured in longitudinal acute or convalescent COVID-19 samples. Thus, reduced sphingosine levels provide a sensitive and selective serologic biomarker for the early identification of asymptomatic versus symptomatic COVID-19 patients.Alhaji H. JannehMohamed Faisal KassirConnor J. DwyerParamita ChakrabortyJason S. PiercePatrick A. FlumeHong LiSatish N. NadigShikhar MehrotraBesim OgretmenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alhaji H. Janneh Mohamed Faisal Kassir Connor J. Dwyer Paramita Chakraborty Jason S. Pierce Patrick A. Flume Hong Li Satish N. Nadig Shikhar Mehrotra Besim Ogretmen Alterations of lipid metabolism provide serologic biomarkers for the detection of asymptomatic versus symptomatic COVID-19 patients
description	Abstract COVID-19 pandemic exerts a health care emergency around the world. The illness severity is heterogeneous. It is mostly unknown why some individuals who are positive for SARS-CoV-2 antibodies stay asymptomatic while others show moderate to severe disease symptoms. Reliable biomarkers for early detection of the disease are urgently needed to attenuate the virus’s spread and help make early treatment decisions. Bioactive sphingolipids play a crucial role in the regulation of viral infections and pro-inflammatory responses involved in the severity of COVID-19. However, any roles of sphingolipids in COVID-19 development or detection remain unknown. In this study, lipidomics measurement of serum sphingolipids demonstrated that reduced sphingosine levels are highly associated with the development of symptomatic COVID-19 in the majority (99.24%) SARS-CoV-2-infected patients compared to asymptomatic counterparts. The majority of asymptomatic individuals (73%) exhibited increased acid ceramidase (AC) in their serum, measured by Western blotting, consistent with elevated sphingosine levels compared to SARS-CoV-2 antibody negative controls. AC protein was also reduced in almost all of the symptomatic patients’ serum, linked to reduced sphingosine levels, measured in longitudinal acute or convalescent COVID-19 samples. Thus, reduced sphingosine levels provide a sensitive and selective serologic biomarker for the early identification of asymptomatic versus symptomatic COVID-19 patients.
format	article
author	Alhaji H. Janneh Mohamed Faisal Kassir Connor J. Dwyer Paramita Chakraborty Jason S. Pierce Patrick A. Flume Hong Li Satish N. Nadig Shikhar Mehrotra Besim Ogretmen
author_facet	Alhaji H. Janneh Mohamed Faisal Kassir Connor J. Dwyer Paramita Chakraborty Jason S. Pierce Patrick A. Flume Hong Li Satish N. Nadig Shikhar Mehrotra Besim Ogretmen
author_sort	Alhaji H. Janneh
title	Alterations of lipid metabolism provide serologic biomarkers for the detection of asymptomatic versus symptomatic COVID-19 patients
title_short	Alterations of lipid metabolism provide serologic biomarkers for the detection of asymptomatic versus symptomatic COVID-19 patients
title_full	Alterations of lipid metabolism provide serologic biomarkers for the detection of asymptomatic versus symptomatic COVID-19 patients
title_fullStr	Alterations of lipid metabolism provide serologic biomarkers for the detection of asymptomatic versus symptomatic COVID-19 patients
title_full_unstemmed	Alterations of lipid metabolism provide serologic biomarkers for the detection of asymptomatic versus symptomatic COVID-19 patients
title_sort	alterations of lipid metabolism provide serologic biomarkers for the detection of asymptomatic versus symptomatic covid-19 patients
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/305a4cbf085d4c1e8de8df3259f7cba4
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Alterations of lipid metabolism provide serologic biomarkers for the detection of asymptomatic versus symptomatic COVID-19 patients

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