Isobaric Tags for Relative and Absolute Quantitation-Based Proteomics Analysis Provides a New Perspective Into Unsynchronized Growth in Kuruma Shrimp (Marsupenaeus japonicus)

Unsynchronized growth is a common phenomenon in farmed crustaceans. The underlying molecular mechanism of unsynchronized growth of crustaceans is unclear. In this study, a comparative proteomic analysis focusing on growth differences was performed using kuruma shrimp Marsupenaeus japonicus, an econo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jichen Zhao, Minze Liao, Zexu Lin, Yiyi Huang, Yunqi Zhong, Luyao Liu, Guoliang Chen, Zuotao Ni, Chengbo Sun
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Marsupenaeus japonicus unsynchronized growth proteomics growth biomarkers molecular mechanism Science Q General. Including nature conservation, geographical distribution QH1-199.5
Acceso en línea:	https://doaj.org/article/305ed4b779ea4077a8e9baae0c552f55
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:305ed4b779ea4077a8e9baae0c552f55
record_format	dspace
spelling	oai:doaj.org-article:305ed4b779ea4077a8e9baae0c552f552021-12-02T10:48:26ZIsobaric Tags for Relative and Absolute Quantitation-Based Proteomics Analysis Provides a New Perspective Into Unsynchronized Growth in Kuruma Shrimp (Marsupenaeus japonicus)2296-774510.3389/fmars.2021.761103https://doaj.org/article/305ed4b779ea4077a8e9baae0c552f552021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmars.2021.761103/fullhttps://doaj.org/toc/2296-7745Unsynchronized growth is a common phenomenon in farmed crustaceans. The underlying molecular mechanism of unsynchronized growth of crustaceans is unclear. In this study, a comparative proteomic analysis focusing on growth differences was performed using kuruma shrimp Marsupenaeus japonicus, an economic crustacean species, as the model. The study analyzed kuruma shrimp at fast growth stage and steady growth stage from both fast growth group and slow growth group by an Isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomic analysis method. A total of 1,720 proteins, including 12,291 peptides, were identified. Fifty-two and 70 differentially expressed proteins (DEPs) were identified in the fast growth stage and steady growth stage, respectively. Interestingly, 10 DEPs, including 14-3-3-epsilon-like, GPI, GPD1, MHC-1a, and MHC-1b, were presented in both growth stages. In addition, all these 10 DEPs shared the same expression tendency at these two growth stages. The results indicated that these 10 DEPs are potential growth biomarkers of M. japonicus. Proteins associated with faster growth of M. japonicus may promote cell growth and inhibit cell apoptosis through the Hippo signaling pathway. The fast growth group of M. japonicus may also achieve growth superiority by activating multiple related metabolic pathways, including glycolysis, glycerophospholipid metabolism and Citrate cycle. The present study provides a new perspective to explore the molecular mechanism of unsynchronized growth in crustacean species.Jichen ZhaoMinze LiaoZexu LinYiyi HuangYunqi ZhongLuyao LiuGuoliang ChenZuotao NiChengbo SunChengbo SunChengbo SunFrontiers Media S.A.articleMarsupenaeus japonicusunsynchronized growthproteomicsgrowth biomarkersmolecular mechanismScienceQGeneral. Including nature conservation, geographical distributionQH1-199.5ENFrontiers in Marine Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Marsupenaeus japonicus unsynchronized growth proteomics growth biomarkers molecular mechanism Science Q General. Including nature conservation, geographical distribution QH1-199.5
spellingShingle	Marsupenaeus japonicus unsynchronized growth proteomics growth biomarkers molecular mechanism Science Q General. Including nature conservation, geographical distribution QH1-199.5 Jichen Zhao Minze Liao Zexu Lin Yiyi Huang Yunqi Zhong Luyao Liu Guoliang Chen Zuotao Ni Chengbo Sun Chengbo Sun Chengbo Sun Isobaric Tags for Relative and Absolute Quantitation-Based Proteomics Analysis Provides a New Perspective Into Unsynchronized Growth in Kuruma Shrimp (Marsupenaeus japonicus)
description	Unsynchronized growth is a common phenomenon in farmed crustaceans. The underlying molecular mechanism of unsynchronized growth of crustaceans is unclear. In this study, a comparative proteomic analysis focusing on growth differences was performed using kuruma shrimp Marsupenaeus japonicus, an economic crustacean species, as the model. The study analyzed kuruma shrimp at fast growth stage and steady growth stage from both fast growth group and slow growth group by an Isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomic analysis method. A total of 1,720 proteins, including 12,291 peptides, were identified. Fifty-two and 70 differentially expressed proteins (DEPs) were identified in the fast growth stage and steady growth stage, respectively. Interestingly, 10 DEPs, including 14-3-3-epsilon-like, GPI, GPD1, MHC-1a, and MHC-1b, were presented in both growth stages. In addition, all these 10 DEPs shared the same expression tendency at these two growth stages. The results indicated that these 10 DEPs are potential growth biomarkers of M. japonicus. Proteins associated with faster growth of M. japonicus may promote cell growth and inhibit cell apoptosis through the Hippo signaling pathway. The fast growth group of M. japonicus may also achieve growth superiority by activating multiple related metabolic pathways, including glycolysis, glycerophospholipid metabolism and Citrate cycle. The present study provides a new perspective to explore the molecular mechanism of unsynchronized growth in crustacean species.
format	article
author	Jichen Zhao Minze Liao Zexu Lin Yiyi Huang Yunqi Zhong Luyao Liu Guoliang Chen Zuotao Ni Chengbo Sun Chengbo Sun Chengbo Sun
author_facet	Jichen Zhao Minze Liao Zexu Lin Yiyi Huang Yunqi Zhong Luyao Liu Guoliang Chen Zuotao Ni Chengbo Sun Chengbo Sun Chengbo Sun
author_sort	Jichen Zhao
title	Isobaric Tags for Relative and Absolute Quantitation-Based Proteomics Analysis Provides a New Perspective Into Unsynchronized Growth in Kuruma Shrimp (Marsupenaeus japonicus)
title_short	Isobaric Tags for Relative and Absolute Quantitation-Based Proteomics Analysis Provides a New Perspective Into Unsynchronized Growth in Kuruma Shrimp (Marsupenaeus japonicus)
title_full	Isobaric Tags for Relative and Absolute Quantitation-Based Proteomics Analysis Provides a New Perspective Into Unsynchronized Growth in Kuruma Shrimp (Marsupenaeus japonicus)
title_fullStr	Isobaric Tags for Relative and Absolute Quantitation-Based Proteomics Analysis Provides a New Perspective Into Unsynchronized Growth in Kuruma Shrimp (Marsupenaeus japonicus)
title_full_unstemmed	Isobaric Tags for Relative and Absolute Quantitation-Based Proteomics Analysis Provides a New Perspective Into Unsynchronized Growth in Kuruma Shrimp (Marsupenaeus japonicus)
title_sort	isobaric tags for relative and absolute quantitation-based proteomics analysis provides a new perspective into unsynchronized growth in kuruma shrimp (marsupenaeus japonicus)
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/305ed4b779ea4077a8e9baae0c552f55
work_keys_str_mv	AT jichenzhao isobarictagsforrelativeandabsolutequantitationbasedproteomicsanalysisprovidesanewperspectiveintounsynchronizedgrowthinkurumashrimpmarsupenaeusjaponicus AT minzeliao isobarictagsforrelativeandabsolutequantitationbasedproteomicsanalysisprovidesanewperspectiveintounsynchronizedgrowthinkurumashrimpmarsupenaeusjaponicus AT zexulin isobarictagsforrelativeandabsolutequantitationbasedproteomicsanalysisprovidesanewperspectiveintounsynchronizedgrowthinkurumashrimpmarsupenaeusjaponicus AT yiyihuang isobarictagsforrelativeandabsolutequantitationbasedproteomicsanalysisprovidesanewperspectiveintounsynchronizedgrowthinkurumashrimpmarsupenaeusjaponicus AT yunqizhong isobarictagsforrelativeandabsolutequantitationbasedproteomicsanalysisprovidesanewperspectiveintounsynchronizedgrowthinkurumashrimpmarsupenaeusjaponicus AT luyaoliu isobarictagsforrelativeandabsolutequantitationbasedproteomicsanalysisprovidesanewperspectiveintounsynchronizedgrowthinkurumashrimpmarsupenaeusjaponicus AT guoliangchen isobarictagsforrelativeandabsolutequantitationbasedproteomicsanalysisprovidesanewperspectiveintounsynchronizedgrowthinkurumashrimpmarsupenaeusjaponicus AT zuotaoni isobarictagsforrelativeandabsolutequantitationbasedproteomicsanalysisprovidesanewperspectiveintounsynchronizedgrowthinkurumashrimpmarsupenaeusjaponicus AT chengbosun isobarictagsforrelativeandabsolutequantitationbasedproteomicsanalysisprovidesanewperspectiveintounsynchronizedgrowthinkurumashrimpmarsupenaeusjaponicus AT chengbosun isobarictagsforrelativeandabsolutequantitationbasedproteomicsanalysisprovidesanewperspectiveintounsynchronizedgrowthinkurumashrimpmarsupenaeusjaponicus AT chengbosun isobarictagsforrelativeandabsolutequantitationbasedproteomicsanalysisprovidesanewperspectiveintounsynchronizedgrowthinkurumashrimpmarsupenaeusjaponicus
_version_	1718396638822662144

Isobaric Tags for Relative and Absolute Quantitation-Based Proteomics Analysis Provides a New Perspective Into Unsynchronized Growth in Kuruma Shrimp (Marsupenaeus japonicus)

Ejemplares similares