Detection of Antibodies Against the SARS-CoV-2 Spike Protein and Analysis of the Peripheral Blood Mononuclear Cell Transcriptomic Profile, 15 Years After Recovery From SARS

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) shows a high degree of homology with SARS-CoV. They share genes, protein sequences, clinical manifestations, and cellular entry patterns. Thus, SARS research may serve helpful in gaining a better understanding of the current coronavirus di...

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Autores principales:	Lili Zhao, Na Han, Yali Zheng, Huiying Rao, Jia Li, Yanwen Chen, Bing Yu, Yu Xu, Hongsong Chen, Zhancheng Gao, Baoguo Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	SARS-CoV SARS-CoV-2 COVID-19 transcriptomic profile immune cross-reactivity Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/306c24868f424da8abfc700f1ab565d0
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spelling	oai:doaj.org-article:306c24868f424da8abfc700f1ab565d02021-11-18T05:44:58ZDetection of Antibodies Against the SARS-CoV-2 Spike Protein and Analysis of the Peripheral Blood Mononuclear Cell Transcriptomic Profile, 15 Years After Recovery From SARS2235-298810.3389/fcimb.2021.768993https://doaj.org/article/306c24868f424da8abfc700f1ab565d02021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.768993/fullhttps://doaj.org/toc/2235-2988Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) shows a high degree of homology with SARS-CoV. They share genes, protein sequences, clinical manifestations, and cellular entry patterns. Thus, SARS research may serve helpful in gaining a better understanding of the current coronavirus disease 2019 (COVID-19) pandemic. Serum antibodies from convalescent patients with SARS collected in 2018 were used to target the recombinant SARS-CoV-2 spike protein via a chemiluminescence microsphere immunoassay. Antibodies of convalescent patients with SARS exhibited serous immune cross-reactivity with the SARS-CoV-2 spike protein. The serous antibodies, excluding S22 of convalescent patients with SARS, did not competitively inhibit the binding of SARS-CoV-2 spike protein to ACE2. T cellular immunity research was conducted in vitro using peripheral blood mononuclear cells (PBMCs) stimulated by pooled peptide epitopes 15 years post-infection. Interferon gamma was detected and the PBMC transcriptomic profile was obtained. The heatmap of the transcriptomic profile showed that mRNAs and circRNAs of the SARS group clustered together after being stimulated by the peptide epitope pool. Differentially expressed mRNAs were most significantly enriched in immunity and signal transduction (P < 0.01). SARS elicits cytokine and chemokine responses, partially consistent with previously published data about COVID-19. Overall, our results indicate that antibodies from convalescent patients with SARS persisted for 15 years and displayed immune cross-reactivity with the SARS-CoV-2 spike protein. The immune status of patients with SARS 15 years post-infection may provide a better understanding of the future immune status of patients with COVID-19.Lili ZhaoNa HanYali ZhengHuiying RaoJia LiYanwen ChenBing YuYu XuHongsong ChenZhancheng GaoBaoguo JiangFrontiers Media S.A.articleSARS-CoVSARS-CoV-2COVID-19transcriptomic profileimmune cross-reactivityMicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SARS-CoV SARS-CoV-2 COVID-19 transcriptomic profile immune cross-reactivity Microbiology QR1-502
spellingShingle	SARS-CoV SARS-CoV-2 COVID-19 transcriptomic profile immune cross-reactivity Microbiology QR1-502 Lili Zhao Na Han Yali Zheng Huiying Rao Jia Li Yanwen Chen Bing Yu Yu Xu Hongsong Chen Zhancheng Gao Baoguo Jiang Detection of Antibodies Against the SARS-CoV-2 Spike Protein and Analysis of the Peripheral Blood Mononuclear Cell Transcriptomic Profile, 15 Years After Recovery From SARS
description	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) shows a high degree of homology with SARS-CoV. They share genes, protein sequences, clinical manifestations, and cellular entry patterns. Thus, SARS research may serve helpful in gaining a better understanding of the current coronavirus disease 2019 (COVID-19) pandemic. Serum antibodies from convalescent patients with SARS collected in 2018 were used to target the recombinant SARS-CoV-2 spike protein via a chemiluminescence microsphere immunoassay. Antibodies of convalescent patients with SARS exhibited serous immune cross-reactivity with the SARS-CoV-2 spike protein. The serous antibodies, excluding S22 of convalescent patients with SARS, did not competitively inhibit the binding of SARS-CoV-2 spike protein to ACE2. T cellular immunity research was conducted in vitro using peripheral blood mononuclear cells (PBMCs) stimulated by pooled peptide epitopes 15 years post-infection. Interferon gamma was detected and the PBMC transcriptomic profile was obtained. The heatmap of the transcriptomic profile showed that mRNAs and circRNAs of the SARS group clustered together after being stimulated by the peptide epitope pool. Differentially expressed mRNAs were most significantly enriched in immunity and signal transduction (P < 0.01). SARS elicits cytokine and chemokine responses, partially consistent with previously published data about COVID-19. Overall, our results indicate that antibodies from convalescent patients with SARS persisted for 15 years and displayed immune cross-reactivity with the SARS-CoV-2 spike protein. The immune status of patients with SARS 15 years post-infection may provide a better understanding of the future immune status of patients with COVID-19.
format	article
author	Lili Zhao Na Han Yali Zheng Huiying Rao Jia Li Yanwen Chen Bing Yu Yu Xu Hongsong Chen Zhancheng Gao Baoguo Jiang
author_facet	Lili Zhao Na Han Yali Zheng Huiying Rao Jia Li Yanwen Chen Bing Yu Yu Xu Hongsong Chen Zhancheng Gao Baoguo Jiang
author_sort	Lili Zhao
title	Detection of Antibodies Against the SARS-CoV-2 Spike Protein and Analysis of the Peripheral Blood Mononuclear Cell Transcriptomic Profile, 15 Years After Recovery From SARS
title_short	Detection of Antibodies Against the SARS-CoV-2 Spike Protein and Analysis of the Peripheral Blood Mononuclear Cell Transcriptomic Profile, 15 Years After Recovery From SARS
title_full	Detection of Antibodies Against the SARS-CoV-2 Spike Protein and Analysis of the Peripheral Blood Mononuclear Cell Transcriptomic Profile, 15 Years After Recovery From SARS
title_fullStr	Detection of Antibodies Against the SARS-CoV-2 Spike Protein and Analysis of the Peripheral Blood Mononuclear Cell Transcriptomic Profile, 15 Years After Recovery From SARS
title_full_unstemmed	Detection of Antibodies Against the SARS-CoV-2 Spike Protein and Analysis of the Peripheral Blood Mononuclear Cell Transcriptomic Profile, 15 Years After Recovery From SARS
title_sort	detection of antibodies against the sars-cov-2 spike protein and analysis of the peripheral blood mononuclear cell transcriptomic profile, 15 years after recovery from sars
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/306c24868f424da8abfc700f1ab565d0
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Detection of Antibodies Against the SARS-CoV-2 Spike Protein and Analysis of the Peripheral Blood Mononuclear Cell Transcriptomic Profile, 15 Years After Recovery From SARS

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