Fabrication of micro-structured scaffold using self-assembled particles and effects of surface geometries on cell adhesion

Controlling of cell location is needed for some cellular applications like drug screening. Micro/nano-structured surface are used for controlling of cell location without using any chemical agents. An average roughness is of interest for investigating an effect on location of cell adhesion. However,...

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Autores principales:	Iwori TAKEDA, Shogo SERIZAWA, Arata KANEKO
Formato:	article
Lenguaje:	EN
Publicado:	The Japan Society of Mechanical Engineers 2016
Materias:	cell adhesion particle reactive ion etching skewness kurtosis Mechanical engineering and machinery TJ1-1570
Acceso en línea:	https://doaj.org/article/307241224d6f455cadcad29ba5036ef6
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spelling	oai:doaj.org-article:307241224d6f455cadcad29ba5036ef62021-11-26T06:35:12ZFabrication of micro-structured scaffold using self-assembled particles and effects of surface geometries on cell adhesion2187-974510.1299/mej.15-00521https://doaj.org/article/307241224d6f455cadcad29ba5036ef62016-01-01T00:00:00Zhttps://www.jstage.jst.go.jp/article/mej/3/1/3_15-00521/_pdf/-char/enhttps://doaj.org/toc/2187-9745Controlling of cell location is needed for some cellular applications like drug screening. Micro/nano-structured surface are used for controlling of cell location without using any chemical agents. An average roughness is of interest for investigating an effect on location of cell adhesion. However, some studies have indicated different results about cell adhesion even though using same kinds of cell line, material properties of scaffolds, and geometrical properties of scaffolds. Those studies have investigated an effect of average roughness only. An average roughness, therefore, is not sufficient for classifying the structured surface. In addition, the structured surfaces have no geometric regularity typically. To resolve these problems, the authors employed regularly arranged surface for cell culture scaffold and investigated effects of an average roughness, skewness, and kurtosis on cell adhesion. The authors used self-assembled SiO2 particles as a mask of reactive ion etching of Si wafer for fabricating micro-structured substrate. Then, geometric transferring technique of polydimethylsiloxane is used for fabricating cell culture scaffolds. An average roughness, skewness, and kurtosis of the scaffolds can be controlled by changing RF power and etching time. The structure that has negative skewness improves cell adhesion was found. It can be seen that Rsk of surface works as an important factor for cell adhesion.Iwori TAKEDAShogo SERIZAWAArata KANEKOThe Japan Society of Mechanical Engineersarticlecell adhesionparticlereactive ion etchingskewnesskurtosisMechanical engineering and machineryTJ1-1570ENMechanical Engineering Journal, Vol 3, Iss 1, Pp 15-00521-15-00521 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	cell adhesion particle reactive ion etching skewness kurtosis Mechanical engineering and machinery TJ1-1570
spellingShingle	cell adhesion particle reactive ion etching skewness kurtosis Mechanical engineering and machinery TJ1-1570 Iwori TAKEDA Shogo SERIZAWA Arata KANEKO Fabrication of micro-structured scaffold using self-assembled particles and effects of surface geometries on cell adhesion
description	Controlling of cell location is needed for some cellular applications like drug screening. Micro/nano-structured surface are used for controlling of cell location without using any chemical agents. An average roughness is of interest for investigating an effect on location of cell adhesion. However, some studies have indicated different results about cell adhesion even though using same kinds of cell line, material properties of scaffolds, and geometrical properties of scaffolds. Those studies have investigated an effect of average roughness only. An average roughness, therefore, is not sufficient for classifying the structured surface. In addition, the structured surfaces have no geometric regularity typically. To resolve these problems, the authors employed regularly arranged surface for cell culture scaffold and investigated effects of an average roughness, skewness, and kurtosis on cell adhesion. The authors used self-assembled SiO2 particles as a mask of reactive ion etching of Si wafer for fabricating micro-structured substrate. Then, geometric transferring technique of polydimethylsiloxane is used for fabricating cell culture scaffolds. An average roughness, skewness, and kurtosis of the scaffolds can be controlled by changing RF power and etching time. The structure that has negative skewness improves cell adhesion was found. It can be seen that Rsk of surface works as an important factor for cell adhesion.
format	article
author	Iwori TAKEDA Shogo SERIZAWA Arata KANEKO
author_facet	Iwori TAKEDA Shogo SERIZAWA Arata KANEKO
author_sort	Iwori TAKEDA
title	Fabrication of micro-structured scaffold using self-assembled particles and effects of surface geometries on cell adhesion
title_short	Fabrication of micro-structured scaffold using self-assembled particles and effects of surface geometries on cell adhesion
title_full	Fabrication of micro-structured scaffold using self-assembled particles and effects of surface geometries on cell adhesion
title_fullStr	Fabrication of micro-structured scaffold using self-assembled particles and effects of surface geometries on cell adhesion
title_full_unstemmed	Fabrication of micro-structured scaffold using self-assembled particles and effects of surface geometries on cell adhesion
title_sort	fabrication of micro-structured scaffold using self-assembled particles and effects of surface geometries on cell adhesion
publisher	The Japan Society of Mechanical Engineers
publishDate	2016
url	https://doaj.org/article/307241224d6f455cadcad29ba5036ef6
work_keys_str_mv	AT iworitakeda fabricationofmicrostructuredscaffoldusingselfassembledparticlesandeffectsofsurfacegeometriesoncelladhesion AT shogoserizawa fabricationofmicrostructuredscaffoldusingselfassembledparticlesandeffectsofsurfacegeometriesoncelladhesion AT aratakaneko fabricationofmicrostructuredscaffoldusingselfassembledparticlesandeffectsofsurfacegeometriesoncelladhesion
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Fabrication of micro-structured scaffold using self-assembled particles and effects of surface geometries on cell adhesion

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