Molecular identification of spiders preying on Empoasca vitis in a tea plantation

Abstract Biological control using predators of key pest species is an attractive option in integrated pest management (IPM). Molecular gut analysis can provide an estimation of predator efficiency on a given prey. Here we use a combination of various experimental approaches, both in field and lab, t...

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Autores principales:	Ting-bang Yang, Jie Liu, Long-yu Yuan, Yang Zhang, Dai-qin Li, Ingi Agnarsson, Jian Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3073a7be972a4b40bfa8355da89bd474
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spelling	oai:doaj.org-article:3073a7be972a4b40bfa8355da89bd4742021-12-02T16:08:09ZMolecular identification of spiders preying on Empoasca vitis in a tea plantation10.1038/s41598-017-07668-w2045-2322https://doaj.org/article/3073a7be972a4b40bfa8355da89bd4742017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07668-whttps://doaj.org/toc/2045-2322Abstract Biological control using predators of key pest species is an attractive option in integrated pest management (IPM). Molecular gut analysis can provide an estimation of predator efficiency on a given prey. Here we use a combination of various experimental approaches, both in field and lab, to identify a potential biological control species of the common pest of commercially grown tea, Empoasca vitis (Göthe) (Hemiptera), in a Chinese plantation. We collected 2655 spiders from plantations and established relative abundances of spider species and their temporal overlap with the pest species in tea canopy. We analyzed DNA from 1363 individuals of the most common spider species using targeted RQ-PCR to quantify the potential efficiency of spiders as a predator on E. vitis. The results showed that, in the field, the jumping spider Evarcha albaria was the most abundant, had the closest temporal overlap with the pest, and frequently fed on it. Therefore, this spider may play a key role in pest suppression. The present study demonstrates the potential of our experimental approach to study predator-prey relationships in taxa that do not lend themselves to morphological identification of gut contents, such as spiders.Ting-bang YangJie LiuLong-yu YuanYang ZhangDai-qin LiIngi AgnarssonJian ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ting-bang Yang Jie Liu Long-yu Yuan Yang Zhang Dai-qin Li Ingi Agnarsson Jian Chen Molecular identification of spiders preying on Empoasca vitis in a tea plantation
description	Abstract Biological control using predators of key pest species is an attractive option in integrated pest management (IPM). Molecular gut analysis can provide an estimation of predator efficiency on a given prey. Here we use a combination of various experimental approaches, both in field and lab, to identify a potential biological control species of the common pest of commercially grown tea, Empoasca vitis (Göthe) (Hemiptera), in a Chinese plantation. We collected 2655 spiders from plantations and established relative abundances of spider species and their temporal overlap with the pest species in tea canopy. We analyzed DNA from 1363 individuals of the most common spider species using targeted RQ-PCR to quantify the potential efficiency of spiders as a predator on E. vitis. The results showed that, in the field, the jumping spider Evarcha albaria was the most abundant, had the closest temporal overlap with the pest, and frequently fed on it. Therefore, this spider may play a key role in pest suppression. The present study demonstrates the potential of our experimental approach to study predator-prey relationships in taxa that do not lend themselves to morphological identification of gut contents, such as spiders.
format	article
author	Ting-bang Yang Jie Liu Long-yu Yuan Yang Zhang Dai-qin Li Ingi Agnarsson Jian Chen
author_facet	Ting-bang Yang Jie Liu Long-yu Yuan Yang Zhang Dai-qin Li Ingi Agnarsson Jian Chen
author_sort	Ting-bang Yang
title	Molecular identification of spiders preying on Empoasca vitis in a tea plantation
title_short	Molecular identification of spiders preying on Empoasca vitis in a tea plantation
title_full	Molecular identification of spiders preying on Empoasca vitis in a tea plantation
title_fullStr	Molecular identification of spiders preying on Empoasca vitis in a tea plantation
title_full_unstemmed	Molecular identification of spiders preying on Empoasca vitis in a tea plantation
title_sort	molecular identification of spiders preying on empoasca vitis in a tea plantation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3073a7be972a4b40bfa8355da89bd474
work_keys_str_mv	AT tingbangyang molecularidentificationofspiderspreyingonempoascavitisinateaplantation AT jieliu molecularidentificationofspiderspreyingonempoascavitisinateaplantation AT longyuyuan molecularidentificationofspiderspreyingonempoascavitisinateaplantation AT yangzhang molecularidentificationofspiderspreyingonempoascavitisinateaplantation AT daiqinli molecularidentificationofspiderspreyingonempoascavitisinateaplantation AT ingiagnarsson molecularidentificationofspiderspreyingonempoascavitisinateaplantation AT jianchen molecularidentificationofspiderspreyingonempoascavitisinateaplantation
_version_	1718384585347170304

Molecular identification of spiders preying on Empoasca vitis in a tea plantation

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