Ricinus communis L. fruit extract inhibits migration/invasion, induces apoptosis in breast cancer cells and arrests tumor progression in vivo

Abstract Medicinal plant-based therapies can be important for treatment of cancer owing to high efficiency, low cost and minimal side effects. Here, we report the anti-cancer efficacy of Ricinus communis L. fruit extract (RCFE) using estrogen positive MCF-7 and highly aggressive, triple negative MDA...

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Autores principales:	Munmi Majumder, Shibjyoti Debnath, Rahul L. Gajbhiye, Rimpi Saikia, Bhaskarjyoti Gogoi, Suman Kumar Samanta, Deepjyoti K. Das, Kaushik Biswas, Parasuraman Jaisankar, Rupak Mukhopadhyay
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/30769e420b854baebbc8aec2517024d7
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spelling	oai:doaj.org-article:30769e420b854baebbc8aec2517024d72021-12-02T15:08:58ZRicinus communis L. fruit extract inhibits migration/invasion, induces apoptosis in breast cancer cells and arrests tumor progression in vivo10.1038/s41598-019-50769-x2045-2322https://doaj.org/article/30769e420b854baebbc8aec2517024d72019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-50769-xhttps://doaj.org/toc/2045-2322Abstract Medicinal plant-based therapies can be important for treatment of cancer owing to high efficiency, low cost and minimal side effects. Here, we report the anti-cancer efficacy of Ricinus communis L. fruit extract (RCFE) using estrogen positive MCF-7 and highly aggressive, triple negative MDA-MB-231 breast cancer cells. RCFE induced cytotoxicity in these cells in dose and time-dependent manner. It also demonstrated robust anti-metastatic activity as it significantly inhibited migration, adhesion, invasion and expression of matrix metalloproteinases (MMPs) 2 and 9 in both cell lines. Further, flow cytometry analysis suggested RCFE-mediated induction of apoptosis in these cells. This was supported by attenuation of anti-apoptotic Bcl-2, induction of pro-apoptotic Bax and caspase-7 expressions as well as PARP cleavage upon RCFE treatment. RCFE (0.5 mg/Kg body weight) treatment led to significant reduction in tumor volume in 4T1 syngeneic mouse model. HPLC and ESI-MS analysis of active ethyl acetate fraction of RCFE detected four compounds, Ricinine, p-Coumaric acid, Epigallocatechin and Ricinoleic acid. Individually these compounds showed cytotoxic and migration-inhibitory activities. Overall, this study for the first time demonstrates the anti-cancer efficacy of the fruit extract of common castor plant which can be proposed as a potent candidate for the treatment of breast cancer.Munmi MajumderShibjyoti DebnathRahul L. GajbhiyeRimpi SaikiaBhaskarjyoti GogoiSuman Kumar SamantaDeepjyoti K. DasKaushik BiswasParasuraman JaisankarRupak MukhopadhyayNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-14 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Munmi Majumder Shibjyoti Debnath Rahul L. Gajbhiye Rimpi Saikia Bhaskarjyoti Gogoi Suman Kumar Samanta Deepjyoti K. Das Kaushik Biswas Parasuraman Jaisankar Rupak Mukhopadhyay Ricinus communis L. fruit extract inhibits migration/invasion, induces apoptosis in breast cancer cells and arrests tumor progression in vivo
description	Abstract Medicinal plant-based therapies can be important for treatment of cancer owing to high efficiency, low cost and minimal side effects. Here, we report the anti-cancer efficacy of Ricinus communis L. fruit extract (RCFE) using estrogen positive MCF-7 and highly aggressive, triple negative MDA-MB-231 breast cancer cells. RCFE induced cytotoxicity in these cells in dose and time-dependent manner. It also demonstrated robust anti-metastatic activity as it significantly inhibited migration, adhesion, invasion and expression of matrix metalloproteinases (MMPs) 2 and 9 in both cell lines. Further, flow cytometry analysis suggested RCFE-mediated induction of apoptosis in these cells. This was supported by attenuation of anti-apoptotic Bcl-2, induction of pro-apoptotic Bax and caspase-7 expressions as well as PARP cleavage upon RCFE treatment. RCFE (0.5 mg/Kg body weight) treatment led to significant reduction in tumor volume in 4T1 syngeneic mouse model. HPLC and ESI-MS analysis of active ethyl acetate fraction of RCFE detected four compounds, Ricinine, p-Coumaric acid, Epigallocatechin and Ricinoleic acid. Individually these compounds showed cytotoxic and migration-inhibitory activities. Overall, this study for the first time demonstrates the anti-cancer efficacy of the fruit extract of common castor plant which can be proposed as a potent candidate for the treatment of breast cancer.
format	article
author	Munmi Majumder Shibjyoti Debnath Rahul L. Gajbhiye Rimpi Saikia Bhaskarjyoti Gogoi Suman Kumar Samanta Deepjyoti K. Das Kaushik Biswas Parasuraman Jaisankar Rupak Mukhopadhyay
author_facet	Munmi Majumder Shibjyoti Debnath Rahul L. Gajbhiye Rimpi Saikia Bhaskarjyoti Gogoi Suman Kumar Samanta Deepjyoti K. Das Kaushik Biswas Parasuraman Jaisankar Rupak Mukhopadhyay
author_sort	Munmi Majumder
title	Ricinus communis L. fruit extract inhibits migration/invasion, induces apoptosis in breast cancer cells and arrests tumor progression in vivo
title_short	Ricinus communis L. fruit extract inhibits migration/invasion, induces apoptosis in breast cancer cells and arrests tumor progression in vivo
title_full	Ricinus communis L. fruit extract inhibits migration/invasion, induces apoptosis in breast cancer cells and arrests tumor progression in vivo
title_fullStr	Ricinus communis L. fruit extract inhibits migration/invasion, induces apoptosis in breast cancer cells and arrests tumor progression in vivo
title_full_unstemmed	Ricinus communis L. fruit extract inhibits migration/invasion, induces apoptosis in breast cancer cells and arrests tumor progression in vivo
title_sort	ricinus communis l. fruit extract inhibits migration/invasion, induces apoptosis in breast cancer cells and arrests tumor progression in vivo
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/30769e420b854baebbc8aec2517024d7
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Ricinus communis L. fruit extract inhibits migration/invasion, induces apoptosis in breast cancer cells and arrests tumor progression in vivo

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