Single-cell transcriptomics of East-Asian pancreatic islets cells

Abstract Single-cell RNA-seq (scRNA-seq) of pancreatic islets have reported on α- and β-cell gene expression in mice and subjects of predominantly European ancestry. We aimed to assess these findings in East-Asian islet-cells. 448 islet-cells were captured from three East-Asian non-diabetic subjects...

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Autores principales:	Rajkumar Dorajoo, Yusuf Ali, Vanessa S. Y. Tay, Jonathan Kang, Sudhagar Samydurai, Jianjun Liu, Bernhard O. Boehm
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3076a127ee5f4b10b3293adc8a34a321
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spelling	oai:doaj.org-article:3076a127ee5f4b10b3293adc8a34a3212021-12-02T11:52:32ZSingle-cell transcriptomics of East-Asian pancreatic islets cells10.1038/s41598-017-05266-42045-2322https://doaj.org/article/3076a127ee5f4b10b3293adc8a34a3212017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05266-4https://doaj.org/toc/2045-2322Abstract Single-cell RNA-seq (scRNA-seq) of pancreatic islets have reported on α- and β-cell gene expression in mice and subjects of predominantly European ancestry. We aimed to assess these findings in East-Asian islet-cells. 448 islet-cells were captured from three East-Asian non-diabetic subjects for scRNA-seq. Hierarchical clustering using pancreatic cell lineage genes was used to assign cells into cell-types. Differentially expressed transcripts between α- and β-cells were detected using ANOVA and in silico replications of mouse and human islet cell genes were performed. We identified 118 α, 105 β, 6 δ endocrine cells and 47 exocrine cells. Besides INS and GCG, 26 genes showed differential expression between α- and β-cells. 10 genes showed concordant expression as reported in rodents, while FAM46A was significantly discordant. Comparing our East-Asian data with data from primarily European subjects, we replicated several genes implicated in nuclear receptor activations, acute phase response pathway, glutaryl-CoA/tryptophan degradations and EIF2/AMPK/mTOR signaling. Additionally, we identified protein ubiquitination to be associated among East-Asian β-cells. We report on East-Asian α- and β-cell gene signatures and substantiate several genes/pathways. We identify expression signatures in East-Asian β-cells that perhaps reflects increased susceptibility to cell-death and warrants future validations to fully appreciate their role in East-Asian diabetes pathogenesis.Rajkumar DorajooYusuf AliVanessa S. Y. TayJonathan KangSudhagar SamyduraiJianjun LiuBernhard O. BoehmNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rajkumar Dorajoo Yusuf Ali Vanessa S. Y. Tay Jonathan Kang Sudhagar Samydurai Jianjun Liu Bernhard O. Boehm Single-cell transcriptomics of East-Asian pancreatic islets cells
description	Abstract Single-cell RNA-seq (scRNA-seq) of pancreatic islets have reported on α- and β-cell gene expression in mice and subjects of predominantly European ancestry. We aimed to assess these findings in East-Asian islet-cells. 448 islet-cells were captured from three East-Asian non-diabetic subjects for scRNA-seq. Hierarchical clustering using pancreatic cell lineage genes was used to assign cells into cell-types. Differentially expressed transcripts between α- and β-cells were detected using ANOVA and in silico replications of mouse and human islet cell genes were performed. We identified 118 α, 105 β, 6 δ endocrine cells and 47 exocrine cells. Besides INS and GCG, 26 genes showed differential expression between α- and β-cells. 10 genes showed concordant expression as reported in rodents, while FAM46A was significantly discordant. Comparing our East-Asian data with data from primarily European subjects, we replicated several genes implicated in nuclear receptor activations, acute phase response pathway, glutaryl-CoA/tryptophan degradations and EIF2/AMPK/mTOR signaling. Additionally, we identified protein ubiquitination to be associated among East-Asian β-cells. We report on East-Asian α- and β-cell gene signatures and substantiate several genes/pathways. We identify expression signatures in East-Asian β-cells that perhaps reflects increased susceptibility to cell-death and warrants future validations to fully appreciate their role in East-Asian diabetes pathogenesis.
format	article
author	Rajkumar Dorajoo Yusuf Ali Vanessa S. Y. Tay Jonathan Kang Sudhagar Samydurai Jianjun Liu Bernhard O. Boehm
author_facet	Rajkumar Dorajoo Yusuf Ali Vanessa S. Y. Tay Jonathan Kang Sudhagar Samydurai Jianjun Liu Bernhard O. Boehm
author_sort	Rajkumar Dorajoo
title	Single-cell transcriptomics of East-Asian pancreatic islets cells
title_short	Single-cell transcriptomics of East-Asian pancreatic islets cells
title_full	Single-cell transcriptomics of East-Asian pancreatic islets cells
title_fullStr	Single-cell transcriptomics of East-Asian pancreatic islets cells
title_full_unstemmed	Single-cell transcriptomics of East-Asian pancreatic islets cells
title_sort	single-cell transcriptomics of east-asian pancreatic islets cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3076a127ee5f4b10b3293adc8a34a321
work_keys_str_mv	AT rajkumardorajoo singlecelltranscriptomicsofeastasianpancreaticisletscells AT yusufali singlecelltranscriptomicsofeastasianpancreaticisletscells AT vanessasytay singlecelltranscriptomicsofeastasianpancreaticisletscells AT jonathankang singlecelltranscriptomicsofeastasianpancreaticisletscells AT sudhagarsamydurai singlecelltranscriptomicsofeastasianpancreaticisletscells AT jianjunliu singlecelltranscriptomicsofeastasianpancreaticisletscells AT bernhardoboehm singlecelltranscriptomicsofeastasianpancreaticisletscells
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Single-cell transcriptomics of East-Asian pancreatic islets cells

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