Insulin-producing cells derived from human embryonic stem cells: comparison of definitive endoderm- and nestin-positive progenitor-based differentiation strategies.

Human embryonic stem cells (hESCs) are pluripotent and capable of undergoing multilineage differentiation into highly specialized cells including pancreatic islet cells. Thus, they represent a novel alternative source for targeted therapies and regenerative medicine for diabetes. Significant progres...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rui Wei, Jin Yang, Wenfang Hou, Guoqiang Liu, Meijuan Gao, Lin Zhang, Haining Wang, Genhong Mao, Hongwei Gao, Guian Chen, Tianpei Hong
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3079976f9e5a47fa8ee0dd429ff63eda
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3079976f9e5a47fa8ee0dd429ff63eda
record_format	dspace
spelling	oai:doaj.org-article:3079976f9e5a47fa8ee0dd429ff63eda2021-11-18T09:00:00ZInsulin-producing cells derived from human embryonic stem cells: comparison of definitive endoderm- and nestin-positive progenitor-based differentiation strategies.1932-620310.1371/journal.pone.0072513https://doaj.org/article/3079976f9e5a47fa8ee0dd429ff63eda2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23951327/?tool=EBIhttps://doaj.org/toc/1932-6203Human embryonic stem cells (hESCs) are pluripotent and capable of undergoing multilineage differentiation into highly specialized cells including pancreatic islet cells. Thus, they represent a novel alternative source for targeted therapies and regenerative medicine for diabetes. Significant progress has been made in differentiating hESCs toward pancreatic lineages. One approach is based on the similarities of pancreatic β cell and neuroepithelial development. Nestin-positive cells are selected as pancreatic β cell precursors and further differentiated to secrete insulin. The other approach is based on our knowledge of developmental biology in which the differentiation protocol sequentially reproduces the individual steps that are known in normal β cell ontogenesis during fetal pancreatic development. In the present study, the hESC cell line PKU1.1 was induced to differentiate into insulin-producing cells (IPCs) using both protocols. The differentiation process was dynamically investigated and the similarities and differences between both strategies were explored. Our results show that IPCs can be successfully induced with both differentiation strategies. The resulting IPCs from both protocols shared many similar features with pancreatic islet cells, but not mature, functional β cells. However, these differently-derived IPC cell types displayed specific morphologies and different expression levels of pancreatic islet development-related markers. These data not only broaden our outlook on hESC differentiation into IPCs, but also extend the full potential of these processes for regenerative medicine in diabetes.Rui WeiJin YangWenfang HouGuoqiang LiuMeijuan GaoLin ZhangHaining WangGenhong MaoHongwei GaoGuian ChenTianpei HongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e72513 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rui Wei Jin Yang Wenfang Hou Guoqiang Liu Meijuan Gao Lin Zhang Haining Wang Genhong Mao Hongwei Gao Guian Chen Tianpei Hong Insulin-producing cells derived from human embryonic stem cells: comparison of definitive endoderm- and nestin-positive progenitor-based differentiation strategies.
description	Human embryonic stem cells (hESCs) are pluripotent and capable of undergoing multilineage differentiation into highly specialized cells including pancreatic islet cells. Thus, they represent a novel alternative source for targeted therapies and regenerative medicine for diabetes. Significant progress has been made in differentiating hESCs toward pancreatic lineages. One approach is based on the similarities of pancreatic β cell and neuroepithelial development. Nestin-positive cells are selected as pancreatic β cell precursors and further differentiated to secrete insulin. The other approach is based on our knowledge of developmental biology in which the differentiation protocol sequentially reproduces the individual steps that are known in normal β cell ontogenesis during fetal pancreatic development. In the present study, the hESC cell line PKU1.1 was induced to differentiate into insulin-producing cells (IPCs) using both protocols. The differentiation process was dynamically investigated and the similarities and differences between both strategies were explored. Our results show that IPCs can be successfully induced with both differentiation strategies. The resulting IPCs from both protocols shared many similar features with pancreatic islet cells, but not mature, functional β cells. However, these differently-derived IPC cell types displayed specific morphologies and different expression levels of pancreatic islet development-related markers. These data not only broaden our outlook on hESC differentiation into IPCs, but also extend the full potential of these processes for regenerative medicine in diabetes.
format	article
author	Rui Wei Jin Yang Wenfang Hou Guoqiang Liu Meijuan Gao Lin Zhang Haining Wang Genhong Mao Hongwei Gao Guian Chen Tianpei Hong
author_facet	Rui Wei Jin Yang Wenfang Hou Guoqiang Liu Meijuan Gao Lin Zhang Haining Wang Genhong Mao Hongwei Gao Guian Chen Tianpei Hong
author_sort	Rui Wei
title	Insulin-producing cells derived from human embryonic stem cells: comparison of definitive endoderm- and nestin-positive progenitor-based differentiation strategies.
title_short	Insulin-producing cells derived from human embryonic stem cells: comparison of definitive endoderm- and nestin-positive progenitor-based differentiation strategies.
title_full	Insulin-producing cells derived from human embryonic stem cells: comparison of definitive endoderm- and nestin-positive progenitor-based differentiation strategies.
title_fullStr	Insulin-producing cells derived from human embryonic stem cells: comparison of definitive endoderm- and nestin-positive progenitor-based differentiation strategies.
title_full_unstemmed	Insulin-producing cells derived from human embryonic stem cells: comparison of definitive endoderm- and nestin-positive progenitor-based differentiation strategies.
title_sort	insulin-producing cells derived from human embryonic stem cells: comparison of definitive endoderm- and nestin-positive progenitor-based differentiation strategies.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/3079976f9e5a47fa8ee0dd429ff63eda
work_keys_str_mv	AT ruiwei insulinproducingcellsderivedfromhumanembryonicstemcellscomparisonofdefinitiveendodermandnestinpositiveprogenitorbaseddifferentiationstrategies AT jinyang insulinproducingcellsderivedfromhumanembryonicstemcellscomparisonofdefinitiveendodermandnestinpositiveprogenitorbaseddifferentiationstrategies AT wenfanghou insulinproducingcellsderivedfromhumanembryonicstemcellscomparisonofdefinitiveendodermandnestinpositiveprogenitorbaseddifferentiationstrategies AT guoqiangliu insulinproducingcellsderivedfromhumanembryonicstemcellscomparisonofdefinitiveendodermandnestinpositiveprogenitorbaseddifferentiationstrategies AT meijuangao insulinproducingcellsderivedfromhumanembryonicstemcellscomparisonofdefinitiveendodermandnestinpositiveprogenitorbaseddifferentiationstrategies AT linzhang insulinproducingcellsderivedfromhumanembryonicstemcellscomparisonofdefinitiveendodermandnestinpositiveprogenitorbaseddifferentiationstrategies AT hainingwang insulinproducingcellsderivedfromhumanembryonicstemcellscomparisonofdefinitiveendodermandnestinpositiveprogenitorbaseddifferentiationstrategies AT genhongmao insulinproducingcellsderivedfromhumanembryonicstemcellscomparisonofdefinitiveendodermandnestinpositiveprogenitorbaseddifferentiationstrategies AT hongweigao insulinproducingcellsderivedfromhumanembryonicstemcellscomparisonofdefinitiveendodermandnestinpositiveprogenitorbaseddifferentiationstrategies AT guianchen insulinproducingcellsderivedfromhumanembryonicstemcellscomparisonofdefinitiveendodermandnestinpositiveprogenitorbaseddifferentiationstrategies AT tianpeihong insulinproducingcellsderivedfromhumanembryonicstemcellscomparisonofdefinitiveendodermandnestinpositiveprogenitorbaseddifferentiationstrategies
_version_	1718421013948006400

Insulin-producing cells derived from human embryonic stem cells: comparison of definitive endoderm- and nestin-positive progenitor-based differentiation strategies.

Ejemplares similares