Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag
Difficulties in imaging non-repetitive genes impair our ability to explore their dynamics. Here the authors present CRISPR-Tag, which marks genes of interest with small repeat sequences to recruit dCas9-GFP.
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| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2018
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/308d55eb091b42a9b2d29f209beb80ba |
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| Sumario: | Difficulties in imaging non-repetitive genes impair our ability to explore their dynamics. Here the authors present CRISPR-Tag, which marks genes of interest with small repeat sequences to recruit dCas9-GFP. |
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