Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag

Difficulties in imaging non-repetitive genes impair our ability to explore their dynamics. Here the authors present CRISPR-Tag, which marks genes of interest with small repeat sequences to recruit dCas9-GFP.

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Autores principales:	Baohui Chen, Wei Zou, Haiyue Xu, Ying Liang, Bo Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/308d55eb091b42a9b2d29f209beb80ba
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spelling	oai:doaj.org-article:308d55eb091b42a9b2d29f209beb80ba2021-12-02T15:33:39ZEfficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag10.1038/s41467-018-07498-y2041-1723https://doaj.org/article/308d55eb091b42a9b2d29f209beb80ba2018-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-07498-yhttps://doaj.org/toc/2041-1723Difficulties in imaging non-repetitive genes impair our ability to explore their dynamics. Here the authors present CRISPR-Tag, which marks genes of interest with small repeat sequences to recruit dCas9-GFP.Baohui ChenWei ZouHaiyue XuYing LiangBo HuangNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Baohui Chen Wei Zou Haiyue Xu Ying Liang Bo Huang Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag
description	Difficulties in imaging non-repetitive genes impair our ability to explore their dynamics. Here the authors present CRISPR-Tag, which marks genes of interest with small repeat sequences to recruit dCas9-GFP.
format	article
author	Baohui Chen Wei Zou Haiyue Xu Ying Liang Bo Huang
author_facet	Baohui Chen Wei Zou Haiyue Xu Ying Liang Bo Huang
author_sort	Baohui Chen
title	Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag
title_short	Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag
title_full	Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag
title_fullStr	Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag
title_full_unstemmed	Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag
title_sort	efficient labeling and imaging of protein-coding genes in living cells using crispr-tag
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/308d55eb091b42a9b2d29f209beb80ba
work_keys_str_mv	AT baohuichen efficientlabelingandimagingofproteincodinggenesinlivingcellsusingcrisprtag AT weizou efficientlabelingandimagingofproteincodinggenesinlivingcellsusingcrisprtag AT haiyuexu efficientlabelingandimagingofproteincodinggenesinlivingcellsusingcrisprtag AT yingliang efficientlabelingandimagingofproteincodinggenesinlivingcellsusingcrisprtag AT bohuang efficientlabelingandimagingofproteincodinggenesinlivingcellsusingcrisprtag
_version_	1718387014704824320

Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag

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