Response sensitivity to bacteriophages of Staphylococcus aureus strains isolated from the microbiota of the oropharynx in children of Irkutsk

Dysbiotic disorders of the oropharynx takes one of the first places in the structure of morbidity worldwide. The aim of the study was to determine the peculiarities of microbiocenosis of the oropharynx in children at different ages and to assess the level of sensitivity of S. aureus strains to speci...

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Autores principales:	E. V. Grigorova, N. M. Voropayeva, U. M. Nemchenko, E. I. Ivanova, E. A. Kungurtseva, T. V. Tunik, L. S. Kozlova
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2017
Materias:	staphylococcus aureus preschool children schoolchildren oropharyngeal microbiocenosis opportunistic pathogens specific bacteriophage Science Q
Acceso en línea:	https://doaj.org/article/30977f1665004e259c7f7f4bcf6fbdfc
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spelling	oai:doaj.org-article:30977f1665004e259c7f7f4bcf6fbdfc2021-11-23T06:14:38ZResponse sensitivity to bacteriophages of Staphylococcus aureus strains isolated from the microbiota of the oropharynx in children of Irkutsk2541-94202587-959610.12737/article_5a3a0dbfab5dc2.42779093https://doaj.org/article/30977f1665004e259c7f7f4bcf6fbdfc2017-09-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/509https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Dysbiotic disorders of the oropharynx takes one of the first places in the structure of morbidity worldwide. The aim of the study was to determine the peculiarities of microbiocenosis of the oropharynx in children at different ages and to assess the level of sensitivity of S. aureus strains to specific bacteriophages. Material for the study consisted of 84 smears from the oropharynx of children with inflammatory diseases of the upper respiratory tract (nasopharyngitis, pharyngitis, tonsillitis, laryngitis (J00, J02, J03, J04 according to 1CD-10). The biological material was distributed depending on the age of the surveyed: the first group - children 3-6 years (n = 61) and the second age group - children 7-12 years (n = 23). Studies of the microflora of the oropharynx were performed according to standard methods. Isolated microorganisms were identified by morphological, tinctorial, cultural and biochemical properties. The results were evaluated in accordance with the Order of USSR Ministry of Health N 535 as of April 22,1985. Specific lytic activity of bacteriophages against S. aureus was determined using preparations of staphylococcal bacteriophage and polyvalent pyobacteriophage Sexta. The sensitivity of strains of S. aureus to specific bacteriophages was assessed by the method of «crosses», according to the guidelines. Statistical analysis was performed with the use of licensed applications Offiœ Ms Excel 2007for Windows 7 BioStat v. 5.0.1.2008. Correlation analysis (Pearson method) was used. 1t has been shown that with increasing age children in the oropharynx microbial density is increasing for indicators of both normoflora (S. oralis), and of opportunistic microflora (S. β-hemolytic, S. pyogenes, S. pneumoniae, bacteria Enterobacteriaceae), especially of S. aureus (up to 74 %). Registration of sensitive strains of S. aureus in more than 70 % of cases in preschool and school-aged children is evidence of the correctness of correction of the dysbiotic disorders of the oropharynx with the use of bacteriophages of staphylococcus and Sexta.E. V. GrigorovaN. M. VoropayevaU. M. NemchenkoE. I. IvanovaE. A. KungurtsevaT. V. TunikL. S. KozlovaScientific Сentre for Family Health and Human Reproduction Problemsarticlestaphylococcus aureuspreschool childrenschoolchildrenoropharyngeal microbiocenosisopportunistic pathogensstaphylococcus aureusspecific bacteriophageScienceQRUActa Biomedica Scientifica, Vol 2, Iss 5(2), Pp 65-69 (2017)
institution	DOAJ
collection	DOAJ
language	RU
topic	staphylococcus aureus preschool children schoolchildren oropharyngeal microbiocenosis opportunistic pathogens staphylococcus aureus specific bacteriophage Science Q
spellingShingle	staphylococcus aureus preschool children schoolchildren oropharyngeal microbiocenosis opportunistic pathogens staphylococcus aureus specific bacteriophage Science Q E. V. Grigorova N. M. Voropayeva U. M. Nemchenko E. I. Ivanova E. A. Kungurtseva T. V. Tunik L. S. Kozlova Response sensitivity to bacteriophages of Staphylococcus aureus strains isolated from the microbiota of the oropharynx in children of Irkutsk
description	Dysbiotic disorders of the oropharynx takes one of the first places in the structure of morbidity worldwide. The aim of the study was to determine the peculiarities of microbiocenosis of the oropharynx in children at different ages and to assess the level of sensitivity of S. aureus strains to specific bacteriophages. Material for the study consisted of 84 smears from the oropharynx of children with inflammatory diseases of the upper respiratory tract (nasopharyngitis, pharyngitis, tonsillitis, laryngitis (J00, J02, J03, J04 according to 1CD-10). The biological material was distributed depending on the age of the surveyed: the first group - children 3-6 years (n = 61) and the second age group - children 7-12 years (n = 23). Studies of the microflora of the oropharynx were performed according to standard methods. Isolated microorganisms were identified by morphological, tinctorial, cultural and biochemical properties. The results were evaluated in accordance with the Order of USSR Ministry of Health N 535 as of April 22,1985. Specific lytic activity of bacteriophages against S. aureus was determined using preparations of staphylococcal bacteriophage and polyvalent pyobacteriophage Sexta. The sensitivity of strains of S. aureus to specific bacteriophages was assessed by the method of «crosses», according to the guidelines. Statistical analysis was performed with the use of licensed applications Offiœ Ms Excel 2007for Windows 7 BioStat v. 5.0.1.2008. Correlation analysis (Pearson method) was used. 1t has been shown that with increasing age children in the oropharynx microbial density is increasing for indicators of both normoflora (S. oralis), and of opportunistic microflora (S. β-hemolytic, S. pyogenes, S. pneumoniae, bacteria Enterobacteriaceae), especially of S. aureus (up to 74 %). Registration of sensitive strains of S. aureus in more than 70 % of cases in preschool and school-aged children is evidence of the correctness of correction of the dysbiotic disorders of the oropharynx with the use of bacteriophages of staphylococcus and Sexta.
format	article
author	E. V. Grigorova N. M. Voropayeva U. M. Nemchenko E. I. Ivanova E. A. Kungurtseva T. V. Tunik L. S. Kozlova
author_facet	E. V. Grigorova N. M. Voropayeva U. M. Nemchenko E. I. Ivanova E. A. Kungurtseva T. V. Tunik L. S. Kozlova
author_sort	E. V. Grigorova
title	Response sensitivity to bacteriophages of Staphylococcus aureus strains isolated from the microbiota of the oropharynx in children of Irkutsk
title_short	Response sensitivity to bacteriophages of Staphylococcus aureus strains isolated from the microbiota of the oropharynx in children of Irkutsk
title_full	Response sensitivity to bacteriophages of Staphylococcus aureus strains isolated from the microbiota of the oropharynx in children of Irkutsk
title_fullStr	Response sensitivity to bacteriophages of Staphylococcus aureus strains isolated from the microbiota of the oropharynx in children of Irkutsk
title_full_unstemmed	Response sensitivity to bacteriophages of Staphylococcus aureus strains isolated from the microbiota of the oropharynx in children of Irkutsk
title_sort	response sensitivity to bacteriophages of staphylococcus aureus strains isolated from the microbiota of the oropharynx in children of irkutsk
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2017
url	https://doaj.org/article/30977f1665004e259c7f7f4bcf6fbdfc
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Response sensitivity to bacteriophages of Staphylococcus aureus strains isolated from the microbiota of the oropharynx in children of Irkutsk

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