Constructing an adult orofacial premotor atlas in Allen mouse CCF

Premotor circuits in the brainstem project to pools of orofacial motoneurons to execute essential motor action such as licking, chewing, breathing, and in rodent, whisking. Previous transsynaptic tracing studies only mapped orofacial premotor circuits in neonatal mice, but the adult circuits remain...

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Autores principales:	Jun Takatoh, Jae Hong Park, Jinghao Lu, Shun Li, PM Thompson, Bao-Xia Han, Shengli Zhao, David Kleinfeld, Beth Friedman, Fan Wang
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	orofacial behaviors premotor circuits whisking licking mastication monosynaptic rabies Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3097c2210f9b4a1dba2bbbd29383a550
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spelling	oai:doaj.org-article:3097c2210f9b4a1dba2bbbd29383a5502021-11-14T15:15:59ZConstructing an adult orofacial premotor atlas in Allen mouse CCF10.7554/eLife.672912050-084Xe67291https://doaj.org/article/3097c2210f9b4a1dba2bbbd29383a5502021-04-01T00:00:00Zhttps://elifesciences.org/articles/67291https://doaj.org/toc/2050-084XPremotor circuits in the brainstem project to pools of orofacial motoneurons to execute essential motor action such as licking, chewing, breathing, and in rodent, whisking. Previous transsynaptic tracing studies only mapped orofacial premotor circuits in neonatal mice, but the adult circuits remain unknown as a consequence of technical difficulties. Here, we developed a three-step monosynaptic transsynaptic tracing strategy to identify premotor neurons controlling vibrissa, tongue protrusion, and jaw-closing muscles in the adult mouse. We registered these different groups of premotor neurons onto the Allen mouse brain common coordinate framework (CCF) and consequently generated a combined 3D orofacial premotor atlas, revealing unique spatial organizations of distinct premotor circuits. We further uncovered premotor neurons that simultaneously innervate multiple motor nuclei and, consequently, are likely to coordinate different muscles involved in the same orofacial motor actions. Our method for tracing adult premotor circuits and registering to Allen CCF is generally applicable and should facilitate the investigations of motor controls of diverse behaviors.Jun TakatohJae Hong ParkJinghao LuShun LiPM ThompsonBao-Xia HanShengli ZhaoDavid KleinfeldBeth FriedmanFan WangeLife Sciences Publications Ltdarticleorofacial behaviorspremotor circuitswhiskinglickingmasticationmonosynaptic rabiesMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	orofacial behaviors premotor circuits whisking licking mastication monosynaptic rabies Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	orofacial behaviors premotor circuits whisking licking mastication monosynaptic rabies Medicine R Science Q Biology (General) QH301-705.5 Jun Takatoh Jae Hong Park Jinghao Lu Shun Li PM Thompson Bao-Xia Han Shengli Zhao David Kleinfeld Beth Friedman Fan Wang Constructing an adult orofacial premotor atlas in Allen mouse CCF
description	Premotor circuits in the brainstem project to pools of orofacial motoneurons to execute essential motor action such as licking, chewing, breathing, and in rodent, whisking. Previous transsynaptic tracing studies only mapped orofacial premotor circuits in neonatal mice, but the adult circuits remain unknown as a consequence of technical difficulties. Here, we developed a three-step monosynaptic transsynaptic tracing strategy to identify premotor neurons controlling vibrissa, tongue protrusion, and jaw-closing muscles in the adult mouse. We registered these different groups of premotor neurons onto the Allen mouse brain common coordinate framework (CCF) and consequently generated a combined 3D orofacial premotor atlas, revealing unique spatial organizations of distinct premotor circuits. We further uncovered premotor neurons that simultaneously innervate multiple motor nuclei and, consequently, are likely to coordinate different muscles involved in the same orofacial motor actions. Our method for tracing adult premotor circuits and registering to Allen CCF is generally applicable and should facilitate the investigations of motor controls of diverse behaviors.
format	article
author	Jun Takatoh Jae Hong Park Jinghao Lu Shun Li PM Thompson Bao-Xia Han Shengli Zhao David Kleinfeld Beth Friedman Fan Wang
author_facet	Jun Takatoh Jae Hong Park Jinghao Lu Shun Li PM Thompson Bao-Xia Han Shengli Zhao David Kleinfeld Beth Friedman Fan Wang
author_sort	Jun Takatoh
title	Constructing an adult orofacial premotor atlas in Allen mouse CCF
title_short	Constructing an adult orofacial premotor atlas in Allen mouse CCF
title_full	Constructing an adult orofacial premotor atlas in Allen mouse CCF
title_fullStr	Constructing an adult orofacial premotor atlas in Allen mouse CCF
title_full_unstemmed	Constructing an adult orofacial premotor atlas in Allen mouse CCF
title_sort	constructing an adult orofacial premotor atlas in allen mouse ccf
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/3097c2210f9b4a1dba2bbbd29383a550
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Constructing an adult orofacial premotor atlas in Allen mouse CCF

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