Transcriptome Comparison of Secondary Metabolite Biosynthesis Genes Expressed in Cultured and Lichenized Conditions of <i>Cladonia rangiferina</i>

Lichen secondary metabolites are natural products of high medicinal and industrial value, which are produced by the fungal symbiont (mycobiont) of lichens in response to environmental changes. It has been shown that the cultured mycobiont is capable of secondary metabolite production, specifically p...

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Autores principales:	Natalia Sveshnikova, Michele D. Piercey-Normore
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Cladonia rangiferina</i> lichen-forming fungi transcriptomics lichen secondary metabolites polyketide synthases PKS Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3098b8201b974c2fa06e6d40e6e838da
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spelling	oai:doaj.org-article:3098b8201b974c2fa06e6d40e6e838da2021-11-25T17:22:25ZTranscriptome Comparison of Secondary Metabolite Biosynthesis Genes Expressed in Cultured and Lichenized Conditions of <i>Cladonia rangiferina</i>10.3390/d131105291424-2818https://doaj.org/article/3098b8201b974c2fa06e6d40e6e838da2021-10-01T00:00:00Zhttps://www.mdpi.com/1424-2818/13/11/529https://doaj.org/toc/1424-2818Lichen secondary metabolites are natural products of high medicinal and industrial value, which are produced by the fungal symbiont (mycobiont) of lichens in response to environmental changes. It has been shown that the cultured mycobiont is capable of secondary metabolite production, specifically polyketides, and polyketide production is affected by the presence or absence of the algal or cyanobacterial symbiont (photobiont). Identification of polyketide synthases encoding genes is, in turn, key for understanding the regulation of secondary metabolite synthesis. Using a previously established method of resynthesis for <i>Cladonia rangiferina</i> as well as the sequenced and assembled genome of that species, we compared transcriptomes of <i>C. rangiferina</i> cultured alone and resynthesized with the photobiont (<i>Asterochloris glomerata</i>) to reveal transcriptionally active genes in secondary metabolic gene clusters, as well some of the neighbouring genes, induced by the presence of the photobiont and events of lichenization. The results identify potential candidates for PKS genes in <i>C. rangiferina</i>, identify potential neighbouring genes in the PKS cluster, and offer insights into further research. The study provides preliminary insights into the activity of several identified biosynthetic gene clusters (BGC) as well as interactions of genes within those clusters.Natalia SveshnikovaMichele D. Piercey-NormoreMDPI AGarticle<i>Cladonia rangiferina</i>lichen-forming fungitranscriptomicslichen secondary metabolitespolyketide synthasesPKSBiology (General)QH301-705.5ENDiversity, Vol 13, Iss 529, p 529 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Cladonia rangiferina</i> lichen-forming fungi transcriptomics lichen secondary metabolites polyketide synthases PKS Biology (General) QH301-705.5
spellingShingle	<i>Cladonia rangiferina</i> lichen-forming fungi transcriptomics lichen secondary metabolites polyketide synthases PKS Biology (General) QH301-705.5 Natalia Sveshnikova Michele D. Piercey-Normore Transcriptome Comparison of Secondary Metabolite Biosynthesis Genes Expressed in Cultured and Lichenized Conditions of <i>Cladonia rangiferina</i>
description	Lichen secondary metabolites are natural products of high medicinal and industrial value, which are produced by the fungal symbiont (mycobiont) of lichens in response to environmental changes. It has been shown that the cultured mycobiont is capable of secondary metabolite production, specifically polyketides, and polyketide production is affected by the presence or absence of the algal or cyanobacterial symbiont (photobiont). Identification of polyketide synthases encoding genes is, in turn, key for understanding the regulation of secondary metabolite synthesis. Using a previously established method of resynthesis for <i>Cladonia rangiferina</i> as well as the sequenced and assembled genome of that species, we compared transcriptomes of <i>C. rangiferina</i> cultured alone and resynthesized with the photobiont (<i>Asterochloris glomerata</i>) to reveal transcriptionally active genes in secondary metabolic gene clusters, as well some of the neighbouring genes, induced by the presence of the photobiont and events of lichenization. The results identify potential candidates for PKS genes in <i>C. rangiferina</i>, identify potential neighbouring genes in the PKS cluster, and offer insights into further research. The study provides preliminary insights into the activity of several identified biosynthetic gene clusters (BGC) as well as interactions of genes within those clusters.
format	article
author	Natalia Sveshnikova Michele D. Piercey-Normore
author_facet	Natalia Sveshnikova Michele D. Piercey-Normore
author_sort	Natalia Sveshnikova
title	Transcriptome Comparison of Secondary Metabolite Biosynthesis Genes Expressed in Cultured and Lichenized Conditions of <i>Cladonia rangiferina</i>
title_short	Transcriptome Comparison of Secondary Metabolite Biosynthesis Genes Expressed in Cultured and Lichenized Conditions of <i>Cladonia rangiferina</i>
title_full	Transcriptome Comparison of Secondary Metabolite Biosynthesis Genes Expressed in Cultured and Lichenized Conditions of <i>Cladonia rangiferina</i>
title_fullStr	Transcriptome Comparison of Secondary Metabolite Biosynthesis Genes Expressed in Cultured and Lichenized Conditions of <i>Cladonia rangiferina</i>
title_full_unstemmed	Transcriptome Comparison of Secondary Metabolite Biosynthesis Genes Expressed in Cultured and Lichenized Conditions of <i>Cladonia rangiferina</i>
title_sort	transcriptome comparison of secondary metabolite biosynthesis genes expressed in cultured and lichenized conditions of <i>cladonia rangiferina</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3098b8201b974c2fa06e6d40e6e838da
work_keys_str_mv	AT nataliasveshnikova transcriptomecomparisonofsecondarymetabolitebiosynthesisgenesexpressedinculturedandlichenizedconditionsoficladoniarangiferinai AT micheledpierceynormore transcriptomecomparisonofsecondarymetabolitebiosynthesisgenesexpressedinculturedandlichenizedconditionsoficladoniarangiferinai
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Transcriptome Comparison of Secondary Metabolite Biosynthesis Genes Expressed in Cultured and Lichenized Conditions of <i>Cladonia rangiferina</i>

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