A hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomers

Liping Sun,1 Yong Zhong,1 Jie Gui,1 Xianwu Wang,1 Xiaorong Zhuang,2 Jian Weng1 1Key Laboratory of Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, College of Materials, Xiamen University, 2Department of Neurology, The Affili...

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Autores principales:	Sun LP, Zhong Y, Gui J, Wang XW, Zhuang XR, Weng J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Alzheimer’s disease amyloid-beta oligomer graphene gold nanoparticles biosensor Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/309b8aaac94b42878e9c3cd814eab571
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spelling	oai:doaj.org-article:309b8aaac94b42878e9c3cd814eab5712021-12-02T02:42:30ZA hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomers1178-2013https://doaj.org/article/309b8aaac94b42878e9c3cd814eab5712018-02-01T00:00:00Zhttps://www.dovepress.com/a-hydrogel-biosensor-for-high-selective-and-sensitive-detection-of-amy-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Liping Sun,1 Yong Zhong,1 Jie Gui,1 Xianwu Wang,1 Xiaorong Zhuang,2 Jian Weng1 1Key Laboratory of Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, College of Materials, Xiamen University, 2Department of Neurology, The Affiliated Zhongshan Hospital of Xiamen University, Xiamen, People’s Republic of China Background: Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by progressive cognitive and memory impairment. It is the most common neurological disease that causes dementia. Soluble amyloid-beta oligomers (AβO) in blood or cerebrospinal fluid (CSF) are the pathogenic biomarker correlated with AD. Methods: A simple electrochemical biosensor using graphene oxide/gold nanoparticles (GNPs) hydrogel electrode was developed in this study. Thiolated cellular prion protein (PrPC) peptide probe was immobilized on GNPs of the hydrogel electrode to construct an AβO biosensor. Electrochemical impedance spectroscopy was utilized for AβO analysis. Results: The specific binding between AβO and PrPC probes on the hydrogel electrode resulted in an increase in the electron-transfer resistance. The biosensor showed high specificity and sensitivity for AβO detection. It could selectively differentiate AβO from amyloid-beta (Aβ) monomers or fibrils. Meanwhile, it was highly sensitive to detect as low as 0.1 pM AβO in artificial CSF or blood plasma. The linear range for AβO detection is from 0.1 pM to 10 nM. Conclusion: This biosensor could be used as a cost-effective tool for early diagnosis of AD due to its high electrochemical performance and bionic structure. Keywords: Alzheimer’s disease, amyloid-beta oligomer, graphene, gold nanoparticles, biosensorSun LPZhong YGui JWang XWZhuang XRWeng JDove Medical PressarticleAlzheimer’s diseaseamyloid-beta oligomergraphenegold nanoparticlesbiosensorMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 843-856 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Alzheimer’s disease amyloid-beta oligomer graphene gold nanoparticles biosensor Medicine (General) R5-920
spellingShingle	Alzheimer’s disease amyloid-beta oligomer graphene gold nanoparticles biosensor Medicine (General) R5-920 Sun LP Zhong Y Gui J Wang XW Zhuang XR Weng J A hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomers
description	Liping Sun,1 Yong Zhong,1 Jie Gui,1 Xianwu Wang,1 Xiaorong Zhuang,2 Jian Weng1 1Key Laboratory of Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, College of Materials, Xiamen University, 2Department of Neurology, The Affiliated Zhongshan Hospital of Xiamen University, Xiamen, People’s Republic of China Background: Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by progressive cognitive and memory impairment. It is the most common neurological disease that causes dementia. Soluble amyloid-beta oligomers (AβO) in blood or cerebrospinal fluid (CSF) are the pathogenic biomarker correlated with AD. Methods: A simple electrochemical biosensor using graphene oxide/gold nanoparticles (GNPs) hydrogel electrode was developed in this study. Thiolated cellular prion protein (PrPC) peptide probe was immobilized on GNPs of the hydrogel electrode to construct an AβO biosensor. Electrochemical impedance spectroscopy was utilized for AβO analysis. Results: The specific binding between AβO and PrPC probes on the hydrogel electrode resulted in an increase in the electron-transfer resistance. The biosensor showed high specificity and sensitivity for AβO detection. It could selectively differentiate AβO from amyloid-beta (Aβ) monomers or fibrils. Meanwhile, it was highly sensitive to detect as low as 0.1 pM AβO in artificial CSF or blood plasma. The linear range for AβO detection is from 0.1 pM to 10 nM. Conclusion: This biosensor could be used as a cost-effective tool for early diagnosis of AD due to its high electrochemical performance and bionic structure. Keywords: Alzheimer’s disease, amyloid-beta oligomer, graphene, gold nanoparticles, biosensor
format	article
author	Sun LP Zhong Y Gui J Wang XW Zhuang XR Weng J
author_facet	Sun LP Zhong Y Gui J Wang XW Zhuang XR Weng J
author_sort	Sun LP
title	A hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomers
title_short	A hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomers
title_full	A hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomers
title_fullStr	A hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomers
title_full_unstemmed	A hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomers
title_sort	hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomers
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/309b8aaac94b42878e9c3cd814eab571
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A hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomers

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