Effect of Glutathione on Sperm Quality in Guanzhong Dairy Goat Sperm During Cryopreservation

In the process of cryopreservation of dairy goat semen, it will face many threats such as oxidative damage, which will affect the motility and plasma membrane function of sperm. As an endogenous antioxidant in animals, glutathione (GSH) can significantly improve the quality of thawed sperm when adde...

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Autores principales:	Jiahao Zou, Lixuan Wei, Dexian Li, Yongtao Zhang, Guang Wang, Lei Zhang, Ping Cao, Shaohua Yang, Guang Li
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Guanzhong dairy goat GSH cryopreservation sperm quality Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/30ad519141964a63a17cb1ab0ceb3d5e
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spelling	oai:doaj.org-article:30ad519141964a63a17cb1ab0ceb3d5e2021-11-18T09:47:39ZEffect of Glutathione on Sperm Quality in Guanzhong Dairy Goat Sperm During Cryopreservation2297-176910.3389/fvets.2021.771440https://doaj.org/article/30ad519141964a63a17cb1ab0ceb3d5e2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.771440/fullhttps://doaj.org/toc/2297-1769In the process of cryopreservation of dairy goat semen, it will face many threats such as oxidative damage, which will affect the motility and plasma membrane function of sperm. As an endogenous antioxidant in animals, glutathione (GSH) can significantly improve the quality of thawed sperm when added to the frozen diluent of semen of pigs and cattle. In this study, different concentration gradients of GSH [0 mmol/L (control), 1, 2, 3, 4 mmol/L] were added to the frozen diluent of Guanzhong dairy goat semen. By detecting the sperm motility parameters, acrosome intact rate and plasma membrane intact rate after thawing, the effect of GSH on the cryopreservation of dairy goat semen was explored. Sperm motility parameters were measured with the computer-aided sperm analysis (CASA) system (total power, TM; forward power, PM; linearity, LIN; average path speed, VAP; straight line speed, VSL; curve speed, VCL; beat cross frequency, BCF). The sperm acrosome integrity rate after thawing was detected by a specific fluorescent probe (isothiocyanate-labeled peanut agglutinin, FITC-PNA), and the sperm plasma membrane integrity rate after thawing was detected by the hypotonic sperm swelling (HOST) method. Reactive oxygen species (ROS) kit, malondialdehyde (MDA) kit, superoxide dismutase (SOD) kit, glutathione peroxidase (GSH-PX) kit were used to detect various antioxidant indicators of thawed sperm. in vitro fertilization experiment was used to verify the effect of adding glutathione on sperm fertilization and embryo development. The results showed that when the concentration of glutathione was 2 mmol/l, the sperm viability, plasma membrane intact rate, and acrosome intact rate were the highest after thawing, reaching 62.14, 37.62, and 70.87% respectively, and they were all significantly higher. In terms of antioxidant indexes; the values of SOD and GSH-PX were 212.60 U/ml and 125.04 U/L, respectively, which were significantly higher than those of the control group; The values of ROS and MDA were 363.05 U/ml and 7.02 nmol/L, respectively, which were significantly lower than the control group. The addition of 2 mmol/L glutathione significantly improves the fertilization ability of sperm. In short, adding 2 mmol/l glutathione to the semen diluent can improve the quality of frozen Guanzhong dairy goat sperm.Jiahao ZouLixuan WeiDexian LiYongtao ZhangGuang WangLei ZhangPing CaoShaohua YangGuang LiFrontiers Media S.A.articleGuanzhong dairy goatGSHcryopreservationspermqualityVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Guanzhong dairy goat GSH cryopreservation sperm quality Veterinary medicine SF600-1100
spellingShingle	Guanzhong dairy goat GSH cryopreservation sperm quality Veterinary medicine SF600-1100 Jiahao Zou Lixuan Wei Dexian Li Yongtao Zhang Guang Wang Lei Zhang Ping Cao Shaohua Yang Guang Li Effect of Glutathione on Sperm Quality in Guanzhong Dairy Goat Sperm During Cryopreservation
description	In the process of cryopreservation of dairy goat semen, it will face many threats such as oxidative damage, which will affect the motility and plasma membrane function of sperm. As an endogenous antioxidant in animals, glutathione (GSH) can significantly improve the quality of thawed sperm when added to the frozen diluent of semen of pigs and cattle. In this study, different concentration gradients of GSH [0 mmol/L (control), 1, 2, 3, 4 mmol/L] were added to the frozen diluent of Guanzhong dairy goat semen. By detecting the sperm motility parameters, acrosome intact rate and plasma membrane intact rate after thawing, the effect of GSH on the cryopreservation of dairy goat semen was explored. Sperm motility parameters were measured with the computer-aided sperm analysis (CASA) system (total power, TM; forward power, PM; linearity, LIN; average path speed, VAP; straight line speed, VSL; curve speed, VCL; beat cross frequency, BCF). The sperm acrosome integrity rate after thawing was detected by a specific fluorescent probe (isothiocyanate-labeled peanut agglutinin, FITC-PNA), and the sperm plasma membrane integrity rate after thawing was detected by the hypotonic sperm swelling (HOST) method. Reactive oxygen species (ROS) kit, malondialdehyde (MDA) kit, superoxide dismutase (SOD) kit, glutathione peroxidase (GSH-PX) kit were used to detect various antioxidant indicators of thawed sperm. in vitro fertilization experiment was used to verify the effect of adding glutathione on sperm fertilization and embryo development. The results showed that when the concentration of glutathione was 2 mmol/l, the sperm viability, plasma membrane intact rate, and acrosome intact rate were the highest after thawing, reaching 62.14, 37.62, and 70.87% respectively, and they were all significantly higher. In terms of antioxidant indexes; the values of SOD and GSH-PX were 212.60 U/ml and 125.04 U/L, respectively, which were significantly higher than those of the control group; The values of ROS and MDA were 363.05 U/ml and 7.02 nmol/L, respectively, which were significantly lower than the control group. The addition of 2 mmol/L glutathione significantly improves the fertilization ability of sperm. In short, adding 2 mmol/l glutathione to the semen diluent can improve the quality of frozen Guanzhong dairy goat sperm.
format	article
author	Jiahao Zou Lixuan Wei Dexian Li Yongtao Zhang Guang Wang Lei Zhang Ping Cao Shaohua Yang Guang Li
author_facet	Jiahao Zou Lixuan Wei Dexian Li Yongtao Zhang Guang Wang Lei Zhang Ping Cao Shaohua Yang Guang Li
author_sort	Jiahao Zou
title	Effect of Glutathione on Sperm Quality in Guanzhong Dairy Goat Sperm During Cryopreservation
title_short	Effect of Glutathione on Sperm Quality in Guanzhong Dairy Goat Sperm During Cryopreservation
title_full	Effect of Glutathione on Sperm Quality in Guanzhong Dairy Goat Sperm During Cryopreservation
title_fullStr	Effect of Glutathione on Sperm Quality in Guanzhong Dairy Goat Sperm During Cryopreservation
title_full_unstemmed	Effect of Glutathione on Sperm Quality in Guanzhong Dairy Goat Sperm During Cryopreservation
title_sort	effect of glutathione on sperm quality in guanzhong dairy goat sperm during cryopreservation
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/30ad519141964a63a17cb1ab0ceb3d5e
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Effect of Glutathione on Sperm Quality in Guanzhong Dairy Goat Sperm During Cryopreservation

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