Effects of symbiotic population impairment on microbiome composition and longevity of <italic toggle="yes">Bactrocera dorsalis</italic>

In order to understand the role of symbionts for their insect hosts, it is customary to treat them with antibiotics or to sterilize eggs (treatments), resulting in aposymbiotic and axenic insects, respectively. Such axenic insects can then be compared with untreated controls. Fruit flies often bear...

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Autores principales:	Mazarin Akami, Ousman Tamgue, Xueming Ren, Yaohui Wang, Xuewei Qi, Koanga Mogtomo Martin Luther, Rosalie Annie Ngono Ngane, Chang-Ying Niu
Formato:	article
Lenguaje:	EN
Publicado:	The Royal Society 2021
Materias:	gut microbiome aposymbiotic axenic longevity Bactrocera dorsalis Science Q
Acceso en línea:	https://doaj.org/article/30b6e795bbf5423c8e902c1d2cfcf363
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spelling	oai:doaj.org-article:30b6e795bbf5423c8e902c1d2cfcf3632021-11-10T08:06:33ZEffects of symbiotic population impairment on microbiome composition and longevity of <italic toggle="yes">Bactrocera dorsalis</italic>10.1098/rsos.2111042054-5703https://doaj.org/article/30b6e795bbf5423c8e902c1d2cfcf3632021-11-01T00:00:00Zhttps://royalsocietypublishing.org/doi/10.1098/rsos.211104https://doaj.org/toc/2054-5703In order to understand the role of symbionts for their insect hosts, it is customary to treat them with antibiotics or to sterilize eggs (treatments), resulting in aposymbiotic and axenic insects, respectively. Such axenic insects can then be compared with untreated controls. Fruit flies often bear complex communities which are greatly reduced by such treatments. However, the bacterial community is not completely eliminated. Here, we examine the effect of these procedures on the structure of the remaining bacterial communities in Bactrocera dorsalis (Diptera: Tephritidae) and on the insect longevity. The antibiotics (Norfloxacin and Ceftazedime) were administered to 1-day-old adult flies through sugar meal for 7 days, and eggs were surface sterilized and dechorionated to produce axenic lines. The flies were starved of protein before they were offered full diets or diets containing non-essential amino acids only. Antibiotic and egg disinfection treatments resulted in a significant reduction of the vast majority of gut bacterial populations, especially Proteobacteria, Firmicutes and Bacteroidetes. On the other hand, antibiotic allowed the persistence of Actinobacteria, Cyanobacteria and Acidobacteria populations. In untreated control flies, longevity was extended irrespective of diet quality in comparison to treated flies. Conversely, when gut bacteria were largely reduced (aposymbiotic and axenic flies), longevity was reduced in the non-essential amino acids diet treatment versus slightly improved in the presence of a protein diet. We discuss these results in an ecological–life-history perspective.Mazarin AkamiOusman TamgueXueming RenYaohui WangXuewei QiKoanga Mogtomo Martin LutherRosalie Annie Ngono NganeChang-Ying NiuThe Royal Societyarticlegut microbiomeaposymbioticaxeniclongevityBactrocera dorsalisScienceQENRoyal Society Open Science, Vol 8, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gut microbiome aposymbiotic axenic longevity Bactrocera dorsalis Science Q
spellingShingle	gut microbiome aposymbiotic axenic longevity Bactrocera dorsalis Science Q Mazarin Akami Ousman Tamgue Xueming Ren Yaohui Wang Xuewei Qi Koanga Mogtomo Martin Luther Rosalie Annie Ngono Ngane Chang-Ying Niu Effects of symbiotic population impairment on microbiome composition and longevity of <italic toggle="yes">Bactrocera dorsalis</italic>
description	In order to understand the role of symbionts for their insect hosts, it is customary to treat them with antibiotics or to sterilize eggs (treatments), resulting in aposymbiotic and axenic insects, respectively. Such axenic insects can then be compared with untreated controls. Fruit flies often bear complex communities which are greatly reduced by such treatments. However, the bacterial community is not completely eliminated. Here, we examine the effect of these procedures on the structure of the remaining bacterial communities in Bactrocera dorsalis (Diptera: Tephritidae) and on the insect longevity. The antibiotics (Norfloxacin and Ceftazedime) were administered to 1-day-old adult flies through sugar meal for 7 days, and eggs were surface sterilized and dechorionated to produce axenic lines. The flies were starved of protein before they were offered full diets or diets containing non-essential amino acids only. Antibiotic and egg disinfection treatments resulted in a significant reduction of the vast majority of gut bacterial populations, especially Proteobacteria, Firmicutes and Bacteroidetes. On the other hand, antibiotic allowed the persistence of Actinobacteria, Cyanobacteria and Acidobacteria populations. In untreated control flies, longevity was extended irrespective of diet quality in comparison to treated flies. Conversely, when gut bacteria were largely reduced (aposymbiotic and axenic flies), longevity was reduced in the non-essential amino acids diet treatment versus slightly improved in the presence of a protein diet. We discuss these results in an ecological–life-history perspective.
format	article
author	Mazarin Akami Ousman Tamgue Xueming Ren Yaohui Wang Xuewei Qi Koanga Mogtomo Martin Luther Rosalie Annie Ngono Ngane Chang-Ying Niu
author_facet	Mazarin Akami Ousman Tamgue Xueming Ren Yaohui Wang Xuewei Qi Koanga Mogtomo Martin Luther Rosalie Annie Ngono Ngane Chang-Ying Niu
author_sort	Mazarin Akami
title	Effects of symbiotic population impairment on microbiome composition and longevity of <italic toggle="yes">Bactrocera dorsalis</italic>
title_short	Effects of symbiotic population impairment on microbiome composition and longevity of <italic toggle="yes">Bactrocera dorsalis</italic>
title_full	Effects of symbiotic population impairment on microbiome composition and longevity of <italic toggle="yes">Bactrocera dorsalis</italic>
title_fullStr	Effects of symbiotic population impairment on microbiome composition and longevity of <italic toggle="yes">Bactrocera dorsalis</italic>
title_full_unstemmed	Effects of symbiotic population impairment on microbiome composition and longevity of <italic toggle="yes">Bactrocera dorsalis</italic>
title_sort	effects of symbiotic population impairment on microbiome composition and longevity of <italic toggle="yes">bactrocera dorsalis</italic>
publisher	The Royal Society
publishDate	2021
url	https://doaj.org/article/30b6e795bbf5423c8e902c1d2cfcf363
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Effects of symbiotic population impairment on microbiome composition and longevity of <italic toggle="yes">Bactrocera dorsalis</italic>

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