Silver nanoparticles enhance the apoptotic potential of gemcitabine in human ovarian cancer cells: combination therapy for effective cancer treatment

Yu-Guo Yuan,1 Qiu-Ling Peng,2 Sangiliyandi Gurunathan3 1College of Veterinary Medicine/Animal Science and Technology/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, People’s Republic of C...

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Autores principales:	Yuan YG, Peng Q, Gurunathan S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Cell viability proliferation cytotoxicity apoptosis gemcitabine silver nanoparticles Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/30baa52299e1456e87b2533405ca29fb
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spelling	oai:doaj.org-article:30baa52299e1456e87b2533405ca29fb2021-12-02T05:04:30ZSilver nanoparticles enhance the apoptotic potential of gemcitabine in human ovarian cancer cells: combination therapy for effective cancer treatment1178-2013https://doaj.org/article/30baa52299e1456e87b2533405ca29fb2017-09-01T00:00:00Zhttps://www.dovepress.com/silver-nanoparticles-enhance-the-apoptotic-potential-of-gemcitabine-in-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yu-Guo Yuan,1 Qiu-Ling Peng,2 Sangiliyandi Gurunathan3 1College of Veterinary Medicine/Animal Science and Technology/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, People’s Republic of China; 2College of Chemistry and Bioengineering, Yichun University, Yichun, Jiangxi, People’s Republic of China; 3Department of Stem cell and Regenerative Biotechnology, Konkuk University, Seoul, Republic of Korea Background: Gemcitabine (GEM) is widely used as an anticancer agent in several types of solid tumors. Silver nanoparticles (AgNPs) possess unique cytotoxic features and can induce apoptosis in a variety of cancer cells. In this study, we investigated whether the combination of GEM and AgNPs can exert synergistic cytotoxic effects in the human ovarian cancer cell line A2780.Methods: We synthesized AgNPs using resveratrol as a reducing and stabilizing agent. The synthesized nanomaterials were characterized using various analytical techniques. The anticancer effects of a combined treatment with GEM and AgNPs were evaluated using a series of cellular assays. The expression of pro- and antiapoptotic genes was measured using real-time reverse transcription polymerase chain reaction. Apoptosis was confirmed by TUNEL assay.Results: In this study, combined treatment with GEM and AgNPs significantly inhibited viability and proliferation in A2780 cells. Moreover, the levels of apoptosis in cells treated with a combination of GEM and AgNPs were significantly higher compared with those in cells treated with GEM or AgNPs alone. Our data suggest that GEM and AgNPs exhibit potent apoptotic activity in human ovarian cancer cells. Combined treatment with GEM and AgNPs showed a significantly higher cytotoxic effect in ovarian cancer cells compared with that induced by either of these agents alone.Conclusion: Our study demonstrated that the interaction between GEM and AgNPs was cytotoxic in ovarian cancer cells. Combined treatment with GEM and AgNPs caused increased cytotoxicity and apoptosis in A2780 cells. This treatment may have therapeutic potential as targeted therapy for the treatment of ovarian cancer. To our knowledge, this study could provide evidence that AgNPs can enhance responsiveness to GEM in ovarian cancer cells and that AgNPs can potentially be used as chemosensitizing agents in ovarian cancer therapy. Keywords: cell viability, proliferation, cytotoxicity, apoptosis, gemcitabine, silver nanoparticlesYuan YGPeng QGurunathan SDove Medical PressarticleCell viabilityproliferationcytotoxicityapoptosisgemcitabinesilver nanoparticlesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 6487-6502 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell viability proliferation cytotoxicity apoptosis gemcitabine silver nanoparticles Medicine (General) R5-920
spellingShingle	Cell viability proliferation cytotoxicity apoptosis gemcitabine silver nanoparticles Medicine (General) R5-920 Yuan YG Peng Q Gurunathan S Silver nanoparticles enhance the apoptotic potential of gemcitabine in human ovarian cancer cells: combination therapy for effective cancer treatment
description	Yu-Guo Yuan,1 Qiu-Ling Peng,2 Sangiliyandi Gurunathan3 1College of Veterinary Medicine/Animal Science and Technology/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, People’s Republic of China; 2College of Chemistry and Bioengineering, Yichun University, Yichun, Jiangxi, People’s Republic of China; 3Department of Stem cell and Regenerative Biotechnology, Konkuk University, Seoul, Republic of Korea Background: Gemcitabine (GEM) is widely used as an anticancer agent in several types of solid tumors. Silver nanoparticles (AgNPs) possess unique cytotoxic features and can induce apoptosis in a variety of cancer cells. In this study, we investigated whether the combination of GEM and AgNPs can exert synergistic cytotoxic effects in the human ovarian cancer cell line A2780.Methods: We synthesized AgNPs using resveratrol as a reducing and stabilizing agent. The synthesized nanomaterials were characterized using various analytical techniques. The anticancer effects of a combined treatment with GEM and AgNPs were evaluated using a series of cellular assays. The expression of pro- and antiapoptotic genes was measured using real-time reverse transcription polymerase chain reaction. Apoptosis was confirmed by TUNEL assay.Results: In this study, combined treatment with GEM and AgNPs significantly inhibited viability and proliferation in A2780 cells. Moreover, the levels of apoptosis in cells treated with a combination of GEM and AgNPs were significantly higher compared with those in cells treated with GEM or AgNPs alone. Our data suggest that GEM and AgNPs exhibit potent apoptotic activity in human ovarian cancer cells. Combined treatment with GEM and AgNPs showed a significantly higher cytotoxic effect in ovarian cancer cells compared with that induced by either of these agents alone.Conclusion: Our study demonstrated that the interaction between GEM and AgNPs was cytotoxic in ovarian cancer cells. Combined treatment with GEM and AgNPs caused increased cytotoxicity and apoptosis in A2780 cells. This treatment may have therapeutic potential as targeted therapy for the treatment of ovarian cancer. To our knowledge, this study could provide evidence that AgNPs can enhance responsiveness to GEM in ovarian cancer cells and that AgNPs can potentially be used as chemosensitizing agents in ovarian cancer therapy. Keywords: cell viability, proliferation, cytotoxicity, apoptosis, gemcitabine, silver nanoparticles
format	article
author	Yuan YG Peng Q Gurunathan S
author_facet	Yuan YG Peng Q Gurunathan S
author_sort	Yuan YG
title	Silver nanoparticles enhance the apoptotic potential of gemcitabine in human ovarian cancer cells: combination therapy for effective cancer treatment
title_short	Silver nanoparticles enhance the apoptotic potential of gemcitabine in human ovarian cancer cells: combination therapy for effective cancer treatment
title_full	Silver nanoparticles enhance the apoptotic potential of gemcitabine in human ovarian cancer cells: combination therapy for effective cancer treatment
title_fullStr	Silver nanoparticles enhance the apoptotic potential of gemcitabine in human ovarian cancer cells: combination therapy for effective cancer treatment
title_full_unstemmed	Silver nanoparticles enhance the apoptotic potential of gemcitabine in human ovarian cancer cells: combination therapy for effective cancer treatment
title_sort	silver nanoparticles enhance the apoptotic potential of gemcitabine in human ovarian cancer cells: combination therapy for effective cancer treatment
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/30baa52299e1456e87b2533405ca29fb
work_keys_str_mv	AT yuanyg silvernanoparticlesenhancetheapoptoticpotentialofgemcitabineinhumanovariancancercellscombinationtherapyforeffectivecancertreatment AT pengq silvernanoparticlesenhancetheapoptoticpotentialofgemcitabineinhumanovariancancercellscombinationtherapyforeffectivecancertreatment AT gurunathans silvernanoparticlesenhancetheapoptoticpotentialofgemcitabineinhumanovariancancercellscombinationtherapyforeffectivecancertreatment
_version_	1718400641397686272

Silver nanoparticles enhance the apoptotic potential of gemcitabine in human ovarian cancer cells: combination therapy for effective cancer treatment

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