Polyphenol containing Sargassum horneri attenuated Th2 differentiation in splenocytes of ovalbumin-sensitised mice: involvement of the transcription factors GATA3/STAT5/NLRP3 in Th2 polarization

Context Sargassum horneri (Turner) C. Agardh (Sargassaceae) is a brown marine alga used in oriental medicine to treat allergic conditions. Objective This study clarifies the effect of polyphenol-containing S. horneri ethanol extract (SHE) on T-helper type-2 (Th2) polarisation. Materials and methods...

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Autores principales:	Kalahe Hewage Iresha Nadeeka Madushani Herath, Jinhee Cho, Hyo Jin Kim, Duong Thi Thuy Dinh, Hyun-Soo Kim, Ginnae Ahn, You-Jin Jeon, Youngheun Jee
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	polyphenol il-13 cd3+ cd4+ t cell Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/30c200aff9874853bc629d6948dc3f67
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spelling	oai:doaj.org-article:30c200aff9874853bc629d6948dc3f672021-11-04T15:00:42ZPolyphenol containing Sargassum horneri attenuated Th2 differentiation in splenocytes of ovalbumin-sensitised mice: involvement of the transcription factors GATA3/STAT5/NLRP3 in Th2 polarization1388-02091744-511610.1080/13880209.2021.1992451https://doaj.org/article/30c200aff9874853bc629d6948dc3f672021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2021.1992451https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context Sargassum horneri (Turner) C. Agardh (Sargassaceae) is a brown marine alga used in oriental medicine to treat allergic conditions. Objective This study clarifies the effect of polyphenol-containing S. horneri ethanol extract (SHE) on T-helper type-2 (Th2) polarisation. Materials and methods All mice (BALB/c mice, n = 12) except in the healthy control group were first sensitised with an intraperitoneal injection of ovalbumin (OVA; 20 µg) and alum (2 mg) on Day 0 and Day 14. Similarly, phosphate-buffered saline (PBS) was injected according to the same schedule into the healthy control mice. After the final administration, splenocytes were obtained. OVA sensitised mice were challenged with OVA (100 µg/mL) in the absence or presence (62.5 and 125 µg/mL) of SHE while healthy control group remained untreated. Results SHE (0–1000 µg/mL) was not cytotoxic to splenocytes and demonstrated IC50 values of 3.27 and 3.92 mg/mL, respectively, at 24 and 48 h of incubation. SHE suppressed cell proliferation at concentrations ≥62.5 µg/mL. SHE treatment (125 µg/mL) subdued (by 1.8-fold) the population expansion of CD3+CD4+ helper T cells induced by OVA challenge. SHE attenuated the OVA-induced activation of respective transcription factors GATA3 and NLRP3. Simultaneously, highly elevated levels of cytokines interleukin (IL)-4 and IL-5 caused by OVA stimulation were removed completely and IL-13 suppressed by 1.5-fold. Conclusions SHE exhibits Th2 immune suppression under OVA stimulation via GATA3- and NLRP3-dependent IL-4, IL-5, and IL-13 suppression. Therefore, SHE could be therapeutically useful for alleviating the symptoms of allergen-mediated immune diseases.Kalahe Hewage Iresha Nadeeka Madushani HerathJinhee ChoHyo Jin KimDuong Thi Thuy DinhHyun-Soo KimGinnae AhnYou-Jin JeonYoungheun JeeTaylor & Francis Grouparticlepolyphenolil-13cd3+ cd4+ t cellTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 59, Iss 1, Pp 1464-1472 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	polyphenol il-13 cd3+ cd4+ t cell Therapeutics. Pharmacology RM1-950
spellingShingle	polyphenol il-13 cd3+ cd4+ t cell Therapeutics. Pharmacology RM1-950 Kalahe Hewage Iresha Nadeeka Madushani Herath Jinhee Cho Hyo Jin Kim Duong Thi Thuy Dinh Hyun-Soo Kim Ginnae Ahn You-Jin Jeon Youngheun Jee Polyphenol containing Sargassum horneri attenuated Th2 differentiation in splenocytes of ovalbumin-sensitised mice: involvement of the transcription factors GATA3/STAT5/NLRP3 in Th2 polarization
description	Context Sargassum horneri (Turner) C. Agardh (Sargassaceae) is a brown marine alga used in oriental medicine to treat allergic conditions. Objective This study clarifies the effect of polyphenol-containing S. horneri ethanol extract (SHE) on T-helper type-2 (Th2) polarisation. Materials and methods All mice (BALB/c mice, n = 12) except in the healthy control group were first sensitised with an intraperitoneal injection of ovalbumin (OVA; 20 µg) and alum (2 mg) on Day 0 and Day 14. Similarly, phosphate-buffered saline (PBS) was injected according to the same schedule into the healthy control mice. After the final administration, splenocytes were obtained. OVA sensitised mice were challenged with OVA (100 µg/mL) in the absence or presence (62.5 and 125 µg/mL) of SHE while healthy control group remained untreated. Results SHE (0–1000 µg/mL) was not cytotoxic to splenocytes and demonstrated IC50 values of 3.27 and 3.92 mg/mL, respectively, at 24 and 48 h of incubation. SHE suppressed cell proliferation at concentrations ≥62.5 µg/mL. SHE treatment (125 µg/mL) subdued (by 1.8-fold) the population expansion of CD3+CD4+ helper T cells induced by OVA challenge. SHE attenuated the OVA-induced activation of respective transcription factors GATA3 and NLRP3. Simultaneously, highly elevated levels of cytokines interleukin (IL)-4 and IL-5 caused by OVA stimulation were removed completely and IL-13 suppressed by 1.5-fold. Conclusions SHE exhibits Th2 immune suppression under OVA stimulation via GATA3- and NLRP3-dependent IL-4, IL-5, and IL-13 suppression. Therefore, SHE could be therapeutically useful for alleviating the symptoms of allergen-mediated immune diseases.
format	article
author	Kalahe Hewage Iresha Nadeeka Madushani Herath Jinhee Cho Hyo Jin Kim Duong Thi Thuy Dinh Hyun-Soo Kim Ginnae Ahn You-Jin Jeon Youngheun Jee
author_facet	Kalahe Hewage Iresha Nadeeka Madushani Herath Jinhee Cho Hyo Jin Kim Duong Thi Thuy Dinh Hyun-Soo Kim Ginnae Ahn You-Jin Jeon Youngheun Jee
author_sort	Kalahe Hewage Iresha Nadeeka Madushani Herath
title	Polyphenol containing Sargassum horneri attenuated Th2 differentiation in splenocytes of ovalbumin-sensitised mice: involvement of the transcription factors GATA3/STAT5/NLRP3 in Th2 polarization
title_short	Polyphenol containing Sargassum horneri attenuated Th2 differentiation in splenocytes of ovalbumin-sensitised mice: involvement of the transcription factors GATA3/STAT5/NLRP3 in Th2 polarization
title_full	Polyphenol containing Sargassum horneri attenuated Th2 differentiation in splenocytes of ovalbumin-sensitised mice: involvement of the transcription factors GATA3/STAT5/NLRP3 in Th2 polarization
title_fullStr	Polyphenol containing Sargassum horneri attenuated Th2 differentiation in splenocytes of ovalbumin-sensitised mice: involvement of the transcription factors GATA3/STAT5/NLRP3 in Th2 polarization
title_full_unstemmed	Polyphenol containing Sargassum horneri attenuated Th2 differentiation in splenocytes of ovalbumin-sensitised mice: involvement of the transcription factors GATA3/STAT5/NLRP3 in Th2 polarization
title_sort	polyphenol containing sargassum horneri attenuated th2 differentiation in splenocytes of ovalbumin-sensitised mice: involvement of the transcription factors gata3/stat5/nlrp3 in th2 polarization
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/30c200aff9874853bc629d6948dc3f67
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Polyphenol containing Sargassum horneri attenuated Th2 differentiation in splenocytes of ovalbumin-sensitised mice: involvement of the transcription factors GATA3/STAT5/NLRP3 in Th2 polarization

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