Temporal and spatial expression of genes involved in DNA methylation during reproductive development of sexual and apomictic Eragrostis curvula

Abstract Recent reports in model plant species have highlighted a role for DNA methylation pathways in the regulation of the somatic-to-reproductive transition in the ovule, suggesting that apomixis (asexual reproduction through seeds) likely relies on RdDM downregulation. Our aim was therefore to e...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	J. P. Selva, L. Siena, J. M. Rodrigo, I. Garbus, D. Zappacosta, J. R. Romero, J. P. A. Ortiz, S. C. Pessino, O. Leblanc, V. Echenique
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/30c32e4771004f6cae8b237d03e291c0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:30c32e4771004f6cae8b237d03e291c0
record_format	dspace
spelling	oai:doaj.org-article:30c32e4771004f6cae8b237d03e291c02021-12-02T11:52:31ZTemporal and spatial expression of genes involved in DNA methylation during reproductive development of sexual and apomictic Eragrostis curvula10.1038/s41598-017-14898-52045-2322https://doaj.org/article/30c32e4771004f6cae8b237d03e291c02017-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-14898-5https://doaj.org/toc/2045-2322Abstract Recent reports in model plant species have highlighted a role for DNA methylation pathways in the regulation of the somatic-to-reproductive transition in the ovule, suggesting that apomixis (asexual reproduction through seeds) likely relies on RdDM downregulation. Our aim was therefore to explore this hypothesis by characterizing genes involved in DNA methylation in the apomictic grass Eragrostis curvula. We explored floral transcriptomes to identify homologs of three candidate genes, for which mutations in Arabidopsis and maize mimic apomixis (AtAGO9/ZmAGO104, AtCMT3/ZmDMT102/ZmDMT105, and AtDDM1/ZmCHR106), and compared both their spatial and temporal expression patterns during reproduction in sexual and apomictic genotypes. Quantitative expression analyses revealed contrasting expression patterns for the three genes in apomictic vs sexual plants. In situ hybridization corroborated these results for two candidates, EcAGO104 and EcDMT102, and revealed an unexpected ectopic pattern for the AGO gene during germ line differentiation in apomicts. Although our data partially support previous results obtained in sexual plant models, they suggest that rather than an RdDM breakdown in the ovule, altered localization of AtAGO9/ZmAGO104 expression is required for achieving diplospory in E. curvula. The differences in the RdDM machinery acquired during plant evolution might have promoted the emergence of the numerous apomictic paths observed in plants.J. P. SelvaL. SienaJ. M. RodrigoI. GarbusD. ZappacostaJ. R. RomeroJ. P. A. OrtizS. C. PessinoO. LeblancV. EcheniqueNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q J. P. Selva L. Siena J. M. Rodrigo I. Garbus D. Zappacosta J. R. Romero J. P. A. Ortiz S. C. Pessino O. Leblanc V. Echenique Temporal and spatial expression of genes involved in DNA methylation during reproductive development of sexual and apomictic Eragrostis curvula
description	Abstract Recent reports in model plant species have highlighted a role for DNA methylation pathways in the regulation of the somatic-to-reproductive transition in the ovule, suggesting that apomixis (asexual reproduction through seeds) likely relies on RdDM downregulation. Our aim was therefore to explore this hypothesis by characterizing genes involved in DNA methylation in the apomictic grass Eragrostis curvula. We explored floral transcriptomes to identify homologs of three candidate genes, for which mutations in Arabidopsis and maize mimic apomixis (AtAGO9/ZmAGO104, AtCMT3/ZmDMT102/ZmDMT105, and AtDDM1/ZmCHR106), and compared both their spatial and temporal expression patterns during reproduction in sexual and apomictic genotypes. Quantitative expression analyses revealed contrasting expression patterns for the three genes in apomictic vs sexual plants. In situ hybridization corroborated these results for two candidates, EcAGO104 and EcDMT102, and revealed an unexpected ectopic pattern for the AGO gene during germ line differentiation in apomicts. Although our data partially support previous results obtained in sexual plant models, they suggest that rather than an RdDM breakdown in the ovule, altered localization of AtAGO9/ZmAGO104 expression is required for achieving diplospory in E. curvula. The differences in the RdDM machinery acquired during plant evolution might have promoted the emergence of the numerous apomictic paths observed in plants.
format	article
author	J. P. Selva L. Siena J. M. Rodrigo I. Garbus D. Zappacosta J. R. Romero J. P. A. Ortiz S. C. Pessino O. Leblanc V. Echenique
author_facet	J. P. Selva L. Siena J. M. Rodrigo I. Garbus D. Zappacosta J. R. Romero J. P. A. Ortiz S. C. Pessino O. Leblanc V. Echenique
author_sort	J. P. Selva
title	Temporal and spatial expression of genes involved in DNA methylation during reproductive development of sexual and apomictic Eragrostis curvula
title_short	Temporal and spatial expression of genes involved in DNA methylation during reproductive development of sexual and apomictic Eragrostis curvula
title_full	Temporal and spatial expression of genes involved in DNA methylation during reproductive development of sexual and apomictic Eragrostis curvula
title_fullStr	Temporal and spatial expression of genes involved in DNA methylation during reproductive development of sexual and apomictic Eragrostis curvula
title_full_unstemmed	Temporal and spatial expression of genes involved in DNA methylation during reproductive development of sexual and apomictic Eragrostis curvula
title_sort	temporal and spatial expression of genes involved in dna methylation during reproductive development of sexual and apomictic eragrostis curvula
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/30c32e4771004f6cae8b237d03e291c0
work_keys_str_mv	AT jpselva temporalandspatialexpressionofgenesinvolvedindnamethylationduringreproductivedevelopmentofsexualandapomicticeragrostiscurvula AT lsiena temporalandspatialexpressionofgenesinvolvedindnamethylationduringreproductivedevelopmentofsexualandapomicticeragrostiscurvula AT jmrodrigo temporalandspatialexpressionofgenesinvolvedindnamethylationduringreproductivedevelopmentofsexualandapomicticeragrostiscurvula AT igarbus temporalandspatialexpressionofgenesinvolvedindnamethylationduringreproductivedevelopmentofsexualandapomicticeragrostiscurvula AT dzappacosta temporalandspatialexpressionofgenesinvolvedindnamethylationduringreproductivedevelopmentofsexualandapomicticeragrostiscurvula AT jrromero temporalandspatialexpressionofgenesinvolvedindnamethylationduringreproductivedevelopmentofsexualandapomicticeragrostiscurvula AT jpaortiz temporalandspatialexpressionofgenesinvolvedindnamethylationduringreproductivedevelopmentofsexualandapomicticeragrostiscurvula AT scpessino temporalandspatialexpressionofgenesinvolvedindnamethylationduringreproductivedevelopmentofsexualandapomicticeragrostiscurvula AT oleblanc temporalandspatialexpressionofgenesinvolvedindnamethylationduringreproductivedevelopmentofsexualandapomicticeragrostiscurvula AT vechenique temporalandspatialexpressionofgenesinvolvedindnamethylationduringreproductivedevelopmentofsexualandapomicticeragrostiscurvula
_version_	1718394974911856640

Temporal and spatial expression of genes involved in DNA methylation during reproductive development of sexual and apomictic Eragrostis curvula

Ejemplares similares