Genomic population structure associated with repeated escape of Salmonella enterica ATCC14028s from the laboratory into nature.

Salmonella enterica serovar Typhimurium strain ATCC14028s is commercially available from multiple national type culture collections, and has been widely used since 1960 for quality control of growth media and experiments on fitness ("laboratory evolution"). ATCC14028s has been implicated i...

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Autores principales:	Mark Achtman, Frederik Van den Broeck, Kerry K Cooper, Philippe Lemey, Craig T Parker, Zhemin Zhou, ATCC14028s Study Group
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/30c8914118044e5f95a234b42b22458f
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spelling	oai:doaj.org-article:30c8914118044e5f95a234b42b22458f2021-12-02T20:03:18ZGenomic population structure associated with repeated escape of Salmonella enterica ATCC14028s from the laboratory into nature.1553-73901553-740410.1371/journal.pgen.1009820https://doaj.org/article/30c8914118044e5f95a234b42b22458f2021-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009820https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Salmonella enterica serovar Typhimurium strain ATCC14028s is commercially available from multiple national type culture collections, and has been widely used since 1960 for quality control of growth media and experiments on fitness ("laboratory evolution"). ATCC14028s has been implicated in multiple cross-contaminations in the laboratory, and has also caused multiple laboratory infections and one known attempt at bioterrorism. According to hierarchical clustering of 3002 core gene sequences, ATCC14028s belongs to HierCC cluster HC20_373 in which most internal branch lengths are only one to three SNPs long. Many natural Typhimurium isolates from humans, domesticated animals and the environment also belong to HC20_373, and their core genomes are almost indistinguishable from those of laboratory strains. These natural isolates have infected humans in Ireland and Taiwan for decades, and are common in the British Isles as well as the Americas. The isolation history of some of the natural isolates confirms the conclusion that they do not represent recent contamination by the laboratory strain, and 10% carry plasmids or bacteriophages which have been acquired in nature by HGT from unrelated bacteria. We propose that ATCC14028s has repeatedly escaped from the laboratory environment into nature via laboratory accidents or infections, but the escaped micro-lineages have only a limited life span. As a result, there is a genetic gap separating HC20_373 from its closest natural relatives due to a divergence between them in the late 19th century followed by repeated extinction events of escaped HC20_373.Mark AchtmanFrederik Van den BroeckKerry K CooperPhilippe LemeyCraig T ParkerZhemin ZhouATCC14028s Study GroupPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 9, p e1009820 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Mark Achtman Frederik Van den Broeck Kerry K Cooper Philippe Lemey Craig T Parker Zhemin Zhou ATCC14028s Study Group Genomic population structure associated with repeated escape of Salmonella enterica ATCC14028s from the laboratory into nature.
description	Salmonella enterica serovar Typhimurium strain ATCC14028s is commercially available from multiple national type culture collections, and has been widely used since 1960 for quality control of growth media and experiments on fitness ("laboratory evolution"). ATCC14028s has been implicated in multiple cross-contaminations in the laboratory, and has also caused multiple laboratory infections and one known attempt at bioterrorism. According to hierarchical clustering of 3002 core gene sequences, ATCC14028s belongs to HierCC cluster HC20_373 in which most internal branch lengths are only one to three SNPs long. Many natural Typhimurium isolates from humans, domesticated animals and the environment also belong to HC20_373, and their core genomes are almost indistinguishable from those of laboratory strains. These natural isolates have infected humans in Ireland and Taiwan for decades, and are common in the British Isles as well as the Americas. The isolation history of some of the natural isolates confirms the conclusion that they do not represent recent contamination by the laboratory strain, and 10% carry plasmids or bacteriophages which have been acquired in nature by HGT from unrelated bacteria. We propose that ATCC14028s has repeatedly escaped from the laboratory environment into nature via laboratory accidents or infections, but the escaped micro-lineages have only a limited life span. As a result, there is a genetic gap separating HC20_373 from its closest natural relatives due to a divergence between them in the late 19th century followed by repeated extinction events of escaped HC20_373.
format	article
author	Mark Achtman Frederik Van den Broeck Kerry K Cooper Philippe Lemey Craig T Parker Zhemin Zhou ATCC14028s Study Group
author_facet	Mark Achtman Frederik Van den Broeck Kerry K Cooper Philippe Lemey Craig T Parker Zhemin Zhou ATCC14028s Study Group
author_sort	Mark Achtman
title	Genomic population structure associated with repeated escape of Salmonella enterica ATCC14028s from the laboratory into nature.
title_short	Genomic population structure associated with repeated escape of Salmonella enterica ATCC14028s from the laboratory into nature.
title_full	Genomic population structure associated with repeated escape of Salmonella enterica ATCC14028s from the laboratory into nature.
title_fullStr	Genomic population structure associated with repeated escape of Salmonella enterica ATCC14028s from the laboratory into nature.
title_full_unstemmed	Genomic population structure associated with repeated escape of Salmonella enterica ATCC14028s from the laboratory into nature.
title_sort	genomic population structure associated with repeated escape of salmonella enterica atcc14028s from the laboratory into nature.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/30c8914118044e5f95a234b42b22458f
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Genomic population structure associated with repeated escape of Salmonella enterica ATCC14028s from the laboratory into nature.

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