Deep-Water Fish Are Potential Vectors of Ciguatera Poisoning in the Gambier Islands, French Polynesia

Ciguatera poisoning (CP) cases linked to the consumption of deep-water fish occurred in 2003 in the Gambier Islands (French Polynesia). In 2004, on the request of two local fishermen, the presence of ciguatoxins (CTXs) was examined in part of their fish catches, i.e., 22 specimens representing five...

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Autores principales:	Hélène Taiana Darius, Taina Revel, Philippe Cruchet, Jérôme Viallon, Clémence Mahana iti Gatti, Manoëlla Sibat, Philipp Hess, Mireille Chinain
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	deep-water fish ciguatera poisoning rRBA MBA CBA-N2a LC–MS/MS Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/30cd613df25e4c17812af42990dcc9da
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spelling	oai:doaj.org-article:30cd613df25e4c17812af42990dcc9da2021-11-25T18:13:03ZDeep-Water Fish Are Potential Vectors of Ciguatera Poisoning in the Gambier Islands, French Polynesia10.3390/md191106441660-3397https://doaj.org/article/30cd613df25e4c17812af42990dcc9da2021-11-01T00:00:00Zhttps://www.mdpi.com/1660-3397/19/11/644https://doaj.org/toc/1660-3397Ciguatera poisoning (CP) cases linked to the consumption of deep-water fish occurred in 2003 in the Gambier Islands (French Polynesia). In 2004, on the request of two local fishermen, the presence of ciguatoxins (CTXs) was examined in part of their fish catches, i.e., 22 specimens representing five deep-water fish species. Using the radioactive receptor binding assay (rRBA) and mouse bioassay (MBA), significant CTX levels were detected in seven deep-water specimens in Lutjanidae, Serranidae, and Bramidae families. Following additional purification steps on the remaining liposoluble fractions for 13 of these samples (kept at −20 °C), these latter were reanalyzed in 2018 with improved protocols of the neuroblastoma cell-based assay (CBA-N2a) and liquid chromatography tandem mass spectrometry (LC–MS/MS). Using the CBA-N2a, the highest CTX-like content found in a specimen of <i>Eumegistus illustris</i> (Bramidae) was 2.94 ± 0.27 µg CTX1B eq. kg<sup>−1</sup>. Its toxin profile consisted of 52-<i>epi</i>-54-deoxyCTX1B, CTX1B, and 54-deoxyCTX1B, as assessed by LC–MS/MS. This is the first study demonstrating that deep-water fish are potential ciguatera vectors and highlighting the importance of a systematic monitoring of CTXs in all exploited fish species, especially in ciguatera hotspots, including deep-water fish, which constitute a significant portion of the commercial deep-sea fisheries in many Asian–Pacific countries.Hélène Taiana DariusTaina RevelPhilippe CruchetJérôme ViallonClémence Mahana iti GattiManoëlla SibatPhilipp HessMireille ChinainMDPI AGarticledeep-water fishciguatera poisoningrRBAMBACBA-N2aLC–MS/MSBiology (General)QH301-705.5ENMarine Drugs, Vol 19, Iss 644, p 644 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	deep-water fish ciguatera poisoning rRBA MBA CBA-N2a LC–MS/MS Biology (General) QH301-705.5
spellingShingle	deep-water fish ciguatera poisoning rRBA MBA CBA-N2a LC–MS/MS Biology (General) QH301-705.5 Hélène Taiana Darius Taina Revel Philippe Cruchet Jérôme Viallon Clémence Mahana iti Gatti Manoëlla Sibat Philipp Hess Mireille Chinain Deep-Water Fish Are Potential Vectors of Ciguatera Poisoning in the Gambier Islands, French Polynesia
description	Ciguatera poisoning (CP) cases linked to the consumption of deep-water fish occurred in 2003 in the Gambier Islands (French Polynesia). In 2004, on the request of two local fishermen, the presence of ciguatoxins (CTXs) was examined in part of their fish catches, i.e., 22 specimens representing five deep-water fish species. Using the radioactive receptor binding assay (rRBA) and mouse bioassay (MBA), significant CTX levels were detected in seven deep-water specimens in Lutjanidae, Serranidae, and Bramidae families. Following additional purification steps on the remaining liposoluble fractions for 13 of these samples (kept at −20 °C), these latter were reanalyzed in 2018 with improved protocols of the neuroblastoma cell-based assay (CBA-N2a) and liquid chromatography tandem mass spectrometry (LC–MS/MS). Using the CBA-N2a, the highest CTX-like content found in a specimen of <i>Eumegistus illustris</i> (Bramidae) was 2.94 ± 0.27 µg CTX1B eq. kg<sup>−1</sup>. Its toxin profile consisted of 52-<i>epi</i>-54-deoxyCTX1B, CTX1B, and 54-deoxyCTX1B, as assessed by LC–MS/MS. This is the first study demonstrating that deep-water fish are potential ciguatera vectors and highlighting the importance of a systematic monitoring of CTXs in all exploited fish species, especially in ciguatera hotspots, including deep-water fish, which constitute a significant portion of the commercial deep-sea fisheries in many Asian–Pacific countries.
format	article
author	Hélène Taiana Darius Taina Revel Philippe Cruchet Jérôme Viallon Clémence Mahana iti Gatti Manoëlla Sibat Philipp Hess Mireille Chinain
author_facet	Hélène Taiana Darius Taina Revel Philippe Cruchet Jérôme Viallon Clémence Mahana iti Gatti Manoëlla Sibat Philipp Hess Mireille Chinain
author_sort	Hélène Taiana Darius
title	Deep-Water Fish Are Potential Vectors of Ciguatera Poisoning in the Gambier Islands, French Polynesia
title_short	Deep-Water Fish Are Potential Vectors of Ciguatera Poisoning in the Gambier Islands, French Polynesia
title_full	Deep-Water Fish Are Potential Vectors of Ciguatera Poisoning in the Gambier Islands, French Polynesia
title_fullStr	Deep-Water Fish Are Potential Vectors of Ciguatera Poisoning in the Gambier Islands, French Polynesia
title_full_unstemmed	Deep-Water Fish Are Potential Vectors of Ciguatera Poisoning in the Gambier Islands, French Polynesia
title_sort	deep-water fish are potential vectors of ciguatera poisoning in the gambier islands, french polynesia
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/30cd613df25e4c17812af42990dcc9da
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Deep-Water Fish Are Potential Vectors of Ciguatera Poisoning in the Gambier Islands, French Polynesia

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