Cell-free prediction of protein expression costs for growing cells

The translation of heterologous proteins places a burden on host cell resources, affecting growth and productivity. Here the authors develop a cell-free assay to measure resource consumption and predict in vivo burden.

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Detalles Bibliográficos
Autores principales: Olivier Borkowski, Carlos Bricio, Michela Murgiano, Brooke Rothschild-Mancinelli, Guy-Bart Stan, Tom Ellis
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/30d5c258a0ca46a1b23db0978ae952b8
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Sumario:The translation of heterologous proteins places a burden on host cell resources, affecting growth and productivity. Here the authors develop a cell-free assay to measure resource consumption and predict in vivo burden.