Cell-free prediction of protein expression costs for growing cells

The translation of heterologous proteins places a burden on host cell resources, affecting growth and productivity. Here the authors develop a cell-free assay to measure resource consumption and predict in vivo burden.

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Auteurs principaux:	Olivier Borkowski, Carlos Bricio, Michela Murgiano, Brooke Rothschild-Mancinelli, Guy-Bart Stan, Tom Ellis
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2018
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/30d5c258a0ca46a1b23db0978ae952b8
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spelling	oai:doaj.org-article:30d5c258a0ca46a1b23db0978ae952b82021-12-02T15:34:49ZCell-free prediction of protein expression costs for growing cells10.1038/s41467-018-03970-x2041-1723https://doaj.org/article/30d5c258a0ca46a1b23db0978ae952b82018-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-03970-xhttps://doaj.org/toc/2041-1723The translation of heterologous proteins places a burden on host cell resources, affecting growth and productivity. Here the authors develop a cell-free assay to measure resource consumption and predict in vivo burden.Olivier BorkowskiCarlos BricioMichela MurgianoBrooke Rothschild-MancinelliGuy-Bart StanTom EllisNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Olivier Borkowski Carlos Bricio Michela Murgiano Brooke Rothschild-Mancinelli Guy-Bart Stan Tom Ellis Cell-free prediction of protein expression costs for growing cells
description	The translation of heterologous proteins places a burden on host cell resources, affecting growth and productivity. Here the authors develop a cell-free assay to measure resource consumption and predict in vivo burden.
format	article
author	Olivier Borkowski Carlos Bricio Michela Murgiano Brooke Rothschild-Mancinelli Guy-Bart Stan Tom Ellis
author_facet	Olivier Borkowski Carlos Bricio Michela Murgiano Brooke Rothschild-Mancinelli Guy-Bart Stan Tom Ellis
author_sort	Olivier Borkowski
title	Cell-free prediction of protein expression costs for growing cells
title_short	Cell-free prediction of protein expression costs for growing cells
title_full	Cell-free prediction of protein expression costs for growing cells
title_fullStr	Cell-free prediction of protein expression costs for growing cells
title_full_unstemmed	Cell-free prediction of protein expression costs for growing cells
title_sort	cell-free prediction of protein expression costs for growing cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/30d5c258a0ca46a1b23db0978ae952b8
work_keys_str_mv	AT olivierborkowski cellfreepredictionofproteinexpressioncostsforgrowingcells AT carlosbricio cellfreepredictionofproteinexpressioncostsforgrowingcells AT michelamurgiano cellfreepredictionofproteinexpressioncostsforgrowingcells AT brookerothschildmancinelli cellfreepredictionofproteinexpressioncostsforgrowingcells AT guybartstan cellfreepredictionofproteinexpressioncostsforgrowingcells AT tomellis cellfreepredictionofproteinexpressioncostsforgrowingcells
_version_	1718386708942159872

Cell-free prediction of protein expression costs for growing cells

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