Cell-free prediction of protein expression costs for growing cells
The translation of heterologous proteins places a burden on host cell resources, affecting growth and productivity. Here the authors develop a cell-free assay to measure resource consumption and predict in vivo burden.
Guardado en:
Autores principales: | , , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2018
|
Materias: | |
Acceso en línea: | https://doaj.org/article/30d5c258a0ca46a1b23db0978ae952b8 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:30d5c258a0ca46a1b23db0978ae952b8 |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:30d5c258a0ca46a1b23db0978ae952b82021-12-02T15:34:49ZCell-free prediction of protein expression costs for growing cells10.1038/s41467-018-03970-x2041-1723https://doaj.org/article/30d5c258a0ca46a1b23db0978ae952b82018-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-03970-xhttps://doaj.org/toc/2041-1723The translation of heterologous proteins places a burden on host cell resources, affecting growth and productivity. Here the authors develop a cell-free assay to measure resource consumption and predict in vivo burden.Olivier BorkowskiCarlos BricioMichela MurgianoBrooke Rothschild-MancinelliGuy-Bart StanTom EllisNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-11 (2018) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Science Q |
spellingShingle |
Science Q Olivier Borkowski Carlos Bricio Michela Murgiano Brooke Rothschild-Mancinelli Guy-Bart Stan Tom Ellis Cell-free prediction of protein expression costs for growing cells |
description |
The translation of heterologous proteins places a burden on host cell resources, affecting growth and productivity. Here the authors develop a cell-free assay to measure resource consumption and predict in vivo burden. |
format |
article |
author |
Olivier Borkowski Carlos Bricio Michela Murgiano Brooke Rothschild-Mancinelli Guy-Bart Stan Tom Ellis |
author_facet |
Olivier Borkowski Carlos Bricio Michela Murgiano Brooke Rothschild-Mancinelli Guy-Bart Stan Tom Ellis |
author_sort |
Olivier Borkowski |
title |
Cell-free prediction of protein expression costs for growing cells |
title_short |
Cell-free prediction of protein expression costs for growing cells |
title_full |
Cell-free prediction of protein expression costs for growing cells |
title_fullStr |
Cell-free prediction of protein expression costs for growing cells |
title_full_unstemmed |
Cell-free prediction of protein expression costs for growing cells |
title_sort |
cell-free prediction of protein expression costs for growing cells |
publisher |
Nature Portfolio |
publishDate |
2018 |
url |
https://doaj.org/article/30d5c258a0ca46a1b23db0978ae952b8 |
work_keys_str_mv |
AT olivierborkowski cellfreepredictionofproteinexpressioncostsforgrowingcells AT carlosbricio cellfreepredictionofproteinexpressioncostsforgrowingcells AT michelamurgiano cellfreepredictionofproteinexpressioncostsforgrowingcells AT brookerothschildmancinelli cellfreepredictionofproteinexpressioncostsforgrowingcells AT guybartstan cellfreepredictionofproteinexpressioncostsforgrowingcells AT tomellis cellfreepredictionofproteinexpressioncostsforgrowingcells |
_version_ |
1718386708942159872 |