Cell-free prediction of protein expression costs for growing cells

The translation of heterologous proteins places a burden on host cell resources, affecting growth and productivity. Here the authors develop a cell-free assay to measure resource consumption and predict in vivo burden.

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Autores principales: Olivier Borkowski, Carlos Bricio, Michela Murgiano, Brooke Rothschild-Mancinelli, Guy-Bart Stan, Tom Ellis
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/30d5c258a0ca46a1b23db0978ae952b8
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spelling oai:doaj.org-article:30d5c258a0ca46a1b23db0978ae952b82021-12-02T15:34:49ZCell-free prediction of protein expression costs for growing cells10.1038/s41467-018-03970-x2041-1723https://doaj.org/article/30d5c258a0ca46a1b23db0978ae952b82018-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-03970-xhttps://doaj.org/toc/2041-1723The translation of heterologous proteins places a burden on host cell resources, affecting growth and productivity. Here the authors develop a cell-free assay to measure resource consumption and predict in vivo burden.Olivier BorkowskiCarlos BricioMichela MurgianoBrooke Rothschild-MancinelliGuy-Bart StanTom EllisNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Olivier Borkowski
Carlos Bricio
Michela Murgiano
Brooke Rothschild-Mancinelli
Guy-Bart Stan
Tom Ellis
Cell-free prediction of protein expression costs for growing cells
description The translation of heterologous proteins places a burden on host cell resources, affecting growth and productivity. Here the authors develop a cell-free assay to measure resource consumption and predict in vivo burden.
format article
author Olivier Borkowski
Carlos Bricio
Michela Murgiano
Brooke Rothschild-Mancinelli
Guy-Bart Stan
Tom Ellis
author_facet Olivier Borkowski
Carlos Bricio
Michela Murgiano
Brooke Rothschild-Mancinelli
Guy-Bart Stan
Tom Ellis
author_sort Olivier Borkowski
title Cell-free prediction of protein expression costs for growing cells
title_short Cell-free prediction of protein expression costs for growing cells
title_full Cell-free prediction of protein expression costs for growing cells
title_fullStr Cell-free prediction of protein expression costs for growing cells
title_full_unstemmed Cell-free prediction of protein expression costs for growing cells
title_sort cell-free prediction of protein expression costs for growing cells
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/30d5c258a0ca46a1b23db0978ae952b8
work_keys_str_mv AT olivierborkowski cellfreepredictionofproteinexpressioncostsforgrowingcells
AT carlosbricio cellfreepredictionofproteinexpressioncostsforgrowingcells
AT michelamurgiano cellfreepredictionofproteinexpressioncostsforgrowingcells
AT brookerothschildmancinelli cellfreepredictionofproteinexpressioncostsforgrowingcells
AT guybartstan cellfreepredictionofproteinexpressioncostsforgrowingcells
AT tomellis cellfreepredictionofproteinexpressioncostsforgrowingcells
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