Cell-free prediction of protein expression costs for growing cells

The translation of heterologous proteins places a burden on host cell resources, affecting growth and productivity. Here the authors develop a cell-free assay to measure resource consumption and predict in vivo burden.

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Auteurs principaux: Olivier Borkowski, Carlos Bricio, Michela Murgiano, Brooke Rothschild-Mancinelli, Guy-Bart Stan, Tom Ellis
Format: article
Langue:EN
Publié: Nature Portfolio 2018
Sujets:
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Accès en ligne:https://doaj.org/article/30d5c258a0ca46a1b23db0978ae952b8
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