Chemical staining of particulate organic matter for improved contrast in soil X-ray µCT images

Abstract Degradability of organic matter (OM) in soil depends on its spatial location in the soil matrix. A recent breakthrough in 3D-localization of OM combined dual-energy X-ray CT-scanning with OsO4 staining of OM. The necessity for synchrotron-based µCT and the use of highly toxic OsO4 severely...

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Autores principales:	Peter Maenhout, Stefaan De Neve, Joanna Wragg, Barry Rawlins, Jan De Pue, Luc Van Hoorebeke, Veerle Cnudde, Steven Sleutel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/30d6de783aeb49489c75cdf14f3164e7
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spelling	oai:doaj.org-article:30d6de783aeb49489c75cdf14f3164e72021-12-02T15:23:01ZChemical staining of particulate organic matter for improved contrast in soil X-ray µCT images10.1038/s41598-020-79681-52045-2322https://doaj.org/article/30d6de783aeb49489c75cdf14f3164e72021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79681-5https://doaj.org/toc/2045-2322Abstract Degradability of organic matter (OM) in soil depends on its spatial location in the soil matrix. A recent breakthrough in 3D-localization of OM combined dual-energy X-ray CT-scanning with OsO4 staining of OM. The necessity for synchrotron-based µCT and the use of highly toxic OsO4 severely limit applications in soil biological experiments. Here, we evaluated the potential of alternative staining agents (silver nitrate, phosphomolybdenic acid (PMA), lead nitrate, lead acetate) to selectively enhance X-ray attenuation and contrast of OM in CT volumes of soils containing specific mineral soil particle fractions, obtained via lab-based X-ray µCT. In comparison with OsO4, administration of Ag+ and Pb2+ resulted in insufficient contrast enhancement of OM versus fine silt (< 20 µm) or clay (< 2 µm) mineral particles. The perfusion procedure used in this work induced changes in soil structure. In contrast, PMA staining resulted in a selective increase of OM’s attenuation contrast, which was comparable to OsO4. However, OM discrimination from other soil phases remained a challenge. Further development of segmentation algorithms accounting for grey value patterns and shape of stained particulate OM may enable its automated identification. If successful in undisturbed soils, PMA staining may form an alternative to OsO4 in non-synchrotron based POM detection.Peter MaenhoutStefaan De NeveJoanna WraggBarry RawlinsJan De PueLuc Van HoorebekeVeerle CnuddeSteven SleutelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peter Maenhout Stefaan De Neve Joanna Wragg Barry Rawlins Jan De Pue Luc Van Hoorebeke Veerle Cnudde Steven Sleutel Chemical staining of particulate organic matter for improved contrast in soil X-ray µCT images
description	Abstract Degradability of organic matter (OM) in soil depends on its spatial location in the soil matrix. A recent breakthrough in 3D-localization of OM combined dual-energy X-ray CT-scanning with OsO4 staining of OM. The necessity for synchrotron-based µCT and the use of highly toxic OsO4 severely limit applications in soil biological experiments. Here, we evaluated the potential of alternative staining agents (silver nitrate, phosphomolybdenic acid (PMA), lead nitrate, lead acetate) to selectively enhance X-ray attenuation and contrast of OM in CT volumes of soils containing specific mineral soil particle fractions, obtained via lab-based X-ray µCT. In comparison with OsO4, administration of Ag+ and Pb2+ resulted in insufficient contrast enhancement of OM versus fine silt (< 20 µm) or clay (< 2 µm) mineral particles. The perfusion procedure used in this work induced changes in soil structure. In contrast, PMA staining resulted in a selective increase of OM’s attenuation contrast, which was comparable to OsO4. However, OM discrimination from other soil phases remained a challenge. Further development of segmentation algorithms accounting for grey value patterns and shape of stained particulate OM may enable its automated identification. If successful in undisturbed soils, PMA staining may form an alternative to OsO4 in non-synchrotron based POM detection.
format	article
author	Peter Maenhout Stefaan De Neve Joanna Wragg Barry Rawlins Jan De Pue Luc Van Hoorebeke Veerle Cnudde Steven Sleutel
author_facet	Peter Maenhout Stefaan De Neve Joanna Wragg Barry Rawlins Jan De Pue Luc Van Hoorebeke Veerle Cnudde Steven Sleutel
author_sort	Peter Maenhout
title	Chemical staining of particulate organic matter for improved contrast in soil X-ray µCT images
title_short	Chemical staining of particulate organic matter for improved contrast in soil X-ray µCT images
title_full	Chemical staining of particulate organic matter for improved contrast in soil X-ray µCT images
title_fullStr	Chemical staining of particulate organic matter for improved contrast in soil X-ray µCT images
title_full_unstemmed	Chemical staining of particulate organic matter for improved contrast in soil X-ray µCT images
title_sort	chemical staining of particulate organic matter for improved contrast in soil x-ray µct images
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/30d6de783aeb49489c75cdf14f3164e7
work_keys_str_mv	AT petermaenhout chemicalstainingofparticulateorganicmatterforimprovedcontrastinsoilxrayμctimages AT stefaandeneve chemicalstainingofparticulateorganicmatterforimprovedcontrastinsoilxrayμctimages AT joannawragg chemicalstainingofparticulateorganicmatterforimprovedcontrastinsoilxrayμctimages AT barryrawlins chemicalstainingofparticulateorganicmatterforimprovedcontrastinsoilxrayμctimages AT jandepue chemicalstainingofparticulateorganicmatterforimprovedcontrastinsoilxrayμctimages AT lucvanhoorebeke chemicalstainingofparticulateorganicmatterforimprovedcontrastinsoilxrayμctimages AT veerlecnudde chemicalstainingofparticulateorganicmatterforimprovedcontrastinsoilxrayμctimages AT stevensleutel chemicalstainingofparticulateorganicmatterforimprovedcontrastinsoilxrayμctimages
_version_	1718387315350437888

Chemical staining of particulate organic matter for improved contrast in soil X-ray µCT images

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