Nicotine-Free e-Cigarette Vapor Exposure Stimulates IL6 and Mucin Production in Human Primary Small Airway Epithelial Cells
Shaan Gellatly, Nicole Pavelka, Taylor Crue, Kelly S Schweitzer, Brian J Day, Elysia Min, Mari Numata, Dennis R Voelker, April Scruggs, Irina Petrache, Hong Wei Chu Department of Medicine, National Jewish Health, Denver, CO 80206, USACorrespondence: Hong Wei Chu; Irina Petrache Tel +1 303-398-1689;...
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Dove Medical Press
2020
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oai:doaj.org-article:30e19d45926541229c87af2dd00fd18b2021-12-02T12:03:52ZNicotine-Free e-Cigarette Vapor Exposure Stimulates IL6 and Mucin Production in Human Primary Small Airway Epithelial Cells1178-7031https://doaj.org/article/30e19d45926541229c87af2dd00fd18b2020-04-01T00:00:00Zhttps://www.dovepress.com/nicotine-free-e-cigarette-vapor-exposure-stimulates-il6-and-mucin-prod-peer-reviewed-article-JIRhttps://doaj.org/toc/1178-7031Shaan Gellatly, Nicole Pavelka, Taylor Crue, Kelly S Schweitzer, Brian J Day, Elysia Min, Mari Numata, Dennis R Voelker, April Scruggs, Irina Petrache, Hong Wei Chu Department of Medicine, National Jewish Health, Denver, CO 80206, USACorrespondence: Hong Wei Chu; Irina Petrache Tel +1 303-398-1689; +1 303-398-2080Fax +1 303-270-2319; +1 303-270-1780Email chuhw@njhealth.org; petrachei@njhealth.orgPurpose: Electronic cigarettes (e-cigs) are relatively new devices that allow the user to inhale a heated and aerosolized solution. At present, little is known about their health effects in the human lung, particularly in the small airways (< 2 mm in diameter), a key site of airway obstruction and destruction in chronic obstructive pulmonary disease and other acute and chronic lung conditions. The aim of this study was to investigate the effect of e-cigarettes on human distal airway inflammation and remodeling.Methods: We isolated primary small airway epithelial cells from donor lungs without known lung disease. Small airway epithelial cells were cultured at air–liquid interface and exposed to 15 puffs vapor obtained by heating a commercially available e-cigarette solution (e-vapor) with or without nicotine. After 24 hrs of e-vapor exposure, basolateral and apical media as well as cell lysates were collected to measure the pleiotropic cytokine interleukin 6 (IL6) and MUC5AC, one of the major components in mucus.Results: Unlike the nicotine-containing e-vapor, nicotine-free e-vapor significantly increased the amount of IL6, which was coupled with increased levels of intracellular MUC5AC protein. Importantly, a neutralizing IL6 antibody (vs an IgG isotype control) significantly inhibited the production of MUC5AC induced by nicotine-free e-vapor.Conclusion: Our results suggest that human small airway epithelial cells exposed to nicotine-free e-vapor increase the inflammatory response and mucin production, which may contribute to distal lung airflow limitation and airway obstruction.Keywords: electronic cigarette, small airway epithelial cells, inflammation, interleukin 6, mucusGellatly SPavelka NCrue TSchweitzer KSDay BJMin ENumata MVoelker DRScruggs APetrache IChu HWDove Medical Pressarticleelectronic cigarettesmall airway epithelial cellsinflammationinterleukin 6mucus. PathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 13, Pp 175-185 (2020) |
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electronic cigarette small airway epithelial cells inflammation interleukin 6 mucus. Pathology RB1-214 Therapeutics. Pharmacology RM1-950 |
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electronic cigarette small airway epithelial cells inflammation interleukin 6 mucus. Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Gellatly S Pavelka N Crue T Schweitzer KS Day BJ Min E Numata M Voelker DR Scruggs A Petrache I Chu HW Nicotine-Free e-Cigarette Vapor Exposure Stimulates IL6 and Mucin Production in Human Primary Small Airway Epithelial Cells |
| description |
Shaan Gellatly, Nicole Pavelka, Taylor Crue, Kelly S Schweitzer, Brian J Day, Elysia Min, Mari Numata, Dennis R Voelker, April Scruggs, Irina Petrache, Hong Wei Chu Department of Medicine, National Jewish Health, Denver, CO 80206, USACorrespondence: Hong Wei Chu; Irina Petrache Tel +1 303-398-1689; +1 303-398-2080Fax +1 303-270-2319; +1 303-270-1780Email chuhw@njhealth.org; petrachei@njhealth.orgPurpose: Electronic cigarettes (e-cigs) are relatively new devices that allow the user to inhale a heated and aerosolized solution. At present, little is known about their health effects in the human lung, particularly in the small airways (< 2 mm in diameter), a key site of airway obstruction and destruction in chronic obstructive pulmonary disease and other acute and chronic lung conditions. The aim of this study was to investigate the effect of e-cigarettes on human distal airway inflammation and remodeling.Methods: We isolated primary small airway epithelial cells from donor lungs without known lung disease. Small airway epithelial cells were cultured at air–liquid interface and exposed to 15 puffs vapor obtained by heating a commercially available e-cigarette solution (e-vapor) with or without nicotine. After 24 hrs of e-vapor exposure, basolateral and apical media as well as cell lysates were collected to measure the pleiotropic cytokine interleukin 6 (IL6) and MUC5AC, one of the major components in mucus.Results: Unlike the nicotine-containing e-vapor, nicotine-free e-vapor significantly increased the amount of IL6, which was coupled with increased levels of intracellular MUC5AC protein. Importantly, a neutralizing IL6 antibody (vs an IgG isotype control) significantly inhibited the production of MUC5AC induced by nicotine-free e-vapor.Conclusion: Our results suggest that human small airway epithelial cells exposed to nicotine-free e-vapor increase the inflammatory response and mucin production, which may contribute to distal lung airflow limitation and airway obstruction.Keywords: electronic cigarette, small airway epithelial cells, inflammation, interleukin 6, mucus |
| format |
article |
| author |
Gellatly S Pavelka N Crue T Schweitzer KS Day BJ Min E Numata M Voelker DR Scruggs A Petrache I Chu HW |
| author_facet |
Gellatly S Pavelka N Crue T Schweitzer KS Day BJ Min E Numata M Voelker DR Scruggs A Petrache I Chu HW |
| author_sort |
Gellatly S |
| title |
Nicotine-Free e-Cigarette Vapor Exposure Stimulates IL6 and Mucin Production in Human Primary Small Airway Epithelial Cells |
| title_short |
Nicotine-Free e-Cigarette Vapor Exposure Stimulates IL6 and Mucin Production in Human Primary Small Airway Epithelial Cells |
| title_full |
Nicotine-Free e-Cigarette Vapor Exposure Stimulates IL6 and Mucin Production in Human Primary Small Airway Epithelial Cells |
| title_fullStr |
Nicotine-Free e-Cigarette Vapor Exposure Stimulates IL6 and Mucin Production in Human Primary Small Airway Epithelial Cells |
| title_full_unstemmed |
Nicotine-Free e-Cigarette Vapor Exposure Stimulates IL6 and Mucin Production in Human Primary Small Airway Epithelial Cells |
| title_sort |
nicotine-free e-cigarette vapor exposure stimulates il6 and mucin production in human primary small airway epithelial cells |
| publisher |
Dove Medical Press |
| publishDate |
2020 |
| url |
https://doaj.org/article/30e19d45926541229c87af2dd00fd18b |
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