The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482

Abstract Pseudomonas donghuensis P482 is a tomato rhizosphere isolate with the ability to inhibit growth of bacterial and fungal plant pathogens. Herein, we analysed the impact of the carbon source on the antibacterial activity of P482 and expression of the selected genes of three genomic regions in...

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Autores principales:	Marta Matuszewska, Tomasz Maciąg, Magdalena Rajewska, Aldona Wierzbicka, Sylwia Jafra
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/30eadd9bd49845c489a2b0baee81da54
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spelling	oai:doaj.org-article:30eadd9bd49845c489a2b0baee81da542021-12-02T14:49:11ZThe carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P48210.1038/s41598-021-90488-w2045-2322https://doaj.org/article/30eadd9bd49845c489a2b0baee81da542021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90488-whttps://doaj.org/toc/2045-2322Abstract Pseudomonas donghuensis P482 is a tomato rhizosphere isolate with the ability to inhibit growth of bacterial and fungal plant pathogens. Herein, we analysed the impact of the carbon source on the antibacterial activity of P482 and expression of the selected genes of three genomic regions in the P482 genome. These regions are involved in the synthesis of pyoverdine, 7-hydroxytropolone (7-HT) and an unknown compound (“cluster 17”) and are responsible for the antimicrobial activity of P482. We showed that the P482 mutants, defective in these regions, show variations and contrasting patterns of growth inhibition of the target pathogen under given nutritional conditions (with glucose or glycerol as a carbon source). We also selected and validated the reference genes for gene expression studies in P. donghuensis P482. Amongst ten candidate genes, we found gyrB, rpoD and mrdA the most stably expressed. Using selected reference genes in RT-qPCR, we assessed the expression of the genes of interest under minimal medium conditions with glucose or glycerol as carbon sources. Glycerol was shown to negatively affect the expression of genes necessary for 7-HT synthesis. The significance of this finding in the light of the role of nutrient (carbon) availability in biological plant protection is discussed.Marta MatuszewskaTomasz MaciągMagdalena RajewskaAldona WierzbickaSylwia JafraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marta Matuszewska Tomasz Maciąg Magdalena Rajewska Aldona Wierzbicka Sylwia Jafra The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482
description	Abstract Pseudomonas donghuensis P482 is a tomato rhizosphere isolate with the ability to inhibit growth of bacterial and fungal plant pathogens. Herein, we analysed the impact of the carbon source on the antibacterial activity of P482 and expression of the selected genes of three genomic regions in the P482 genome. These regions are involved in the synthesis of pyoverdine, 7-hydroxytropolone (7-HT) and an unknown compound (“cluster 17”) and are responsible for the antimicrobial activity of P482. We showed that the P482 mutants, defective in these regions, show variations and contrasting patterns of growth inhibition of the target pathogen under given nutritional conditions (with glucose or glycerol as a carbon source). We also selected and validated the reference genes for gene expression studies in P. donghuensis P482. Amongst ten candidate genes, we found gyrB, rpoD and mrdA the most stably expressed. Using selected reference genes in RT-qPCR, we assessed the expression of the genes of interest under minimal medium conditions with glucose or glycerol as carbon sources. Glycerol was shown to negatively affect the expression of genes necessary for 7-HT synthesis. The significance of this finding in the light of the role of nutrient (carbon) availability in biological plant protection is discussed.
format	article
author	Marta Matuszewska Tomasz Maciąg Magdalena Rajewska Aldona Wierzbicka Sylwia Jafra
author_facet	Marta Matuszewska Tomasz Maciąg Magdalena Rajewska Aldona Wierzbicka Sylwia Jafra
author_sort	Marta Matuszewska
title	The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482
title_short	The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482
title_full	The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482
title_fullStr	The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482
title_full_unstemmed	The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482
title_sort	carbon source-dependent pattern of antimicrobial activity and gene expression in pseudomonas donghuensis p482
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/30eadd9bd49845c489a2b0baee81da54
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The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482

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