MiR-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets

ABSTRACT: Several recent studies investigated the role of the miR-29 family in muscle development. However, only a few studies focused on chicken skeletal muscle. In the present study, cell cycle, 5-ethynyl-2’-deoxyuridine (EdU), cell counting kit-8 (CCK-8), and other assays indicated that miR-29b-1...

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Autores principales: Yuanfang Li, Bin Zhai, Pengtao Yuan, Shengxin Fan, Wenjiao Jin, Wenting Li, Guirong Sun, Yadong Tian, Xiaojun Liu, Xiangtao Kang, Guoxi Li
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Publicado: Elsevier 2022
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spelling oai:doaj.org-article:30f150d5cf114bf598999a41728487c62021-11-30T04:13:33ZMiR-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets0032-579110.1016/j.psj.2021.101557https://doaj.org/article/30f150d5cf114bf598999a41728487c62022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121005794https://doaj.org/toc/0032-5791ABSTRACT: Several recent studies investigated the role of the miR-29 family in muscle development. However, only a few studies focused on chicken skeletal muscle. In the present study, cell cycle, 5-ethynyl-2’-deoxyuridine (EdU), cell counting kit-8 (CCK-8), and other assays indicated that miR-29b-1-5p can inhibit the proliferation of chicken primary myoblasts (CPMs); the western blot assay and immunofluorescence detection of MYHC demonstrated that miR-29b-1-5p can promote the differentiation of myoblasts. The functional enrichment analysis revealed that the target genes of miR-29b-1-5p may be involved in muscle tissue development, muscle organ development, and striated muscle tissue development, which are biological processes related to muscle development. The correlation analysis showed that these 6 genes, that is, ankyrin repeat domain 9 (ANKRD9), lactate dehydrogenase A (LDHA), transcription factor 12 (TCF12), FAT atypical cadherin 1 (FAT1), lin-9 homolog (LIN9), and integrin beta 3 binding protein (ITGB3BP), can be used as effective candidate target genes of miR-29b-1-5p. Moreover, miR-29b-1-5p inhibits the expression of ANKRD9 by directly binding the 3′UTR of ANKRD9. Overall, these data indicate that miR-29b-1-5p inhibits the proliferation of primary chicken myoblasts, stimulates their differentiation, and is involved in the process of muscle development and that its effective target gene is ANKRD9. This study identified the molecular mechanism of miR-29b-1-5p in chicken muscle development.Yuanfang LiBin ZhaiPengtao YuanShengxin FanWenjiao JinWenting LiGuirong SunYadong TianXiaojun LiuXiangtao KangGuoxi LiElsevierarticlemiR-29b-1-5pANKRD9chicken primary myoblastmuscle developmentAnimal cultureSF1-1100ENPoultry Science, Vol 101, Iss 2, Pp 101557- (2022)
institution DOAJ
collection DOAJ
language EN
topic miR-29b-1-5p
ANKRD9
chicken primary myoblast
muscle development
Animal culture
SF1-1100
spellingShingle miR-29b-1-5p
ANKRD9
chicken primary myoblast
muscle development
Animal culture
SF1-1100
Yuanfang Li
Bin Zhai
Pengtao Yuan
Shengxin Fan
Wenjiao Jin
Wenting Li
Guirong Sun
Yadong Tian
Xiaojun Liu
Xiangtao Kang
Guoxi Li
MiR-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets
description ABSTRACT: Several recent studies investigated the role of the miR-29 family in muscle development. However, only a few studies focused on chicken skeletal muscle. In the present study, cell cycle, 5-ethynyl-2’-deoxyuridine (EdU), cell counting kit-8 (CCK-8), and other assays indicated that miR-29b-1-5p can inhibit the proliferation of chicken primary myoblasts (CPMs); the western blot assay and immunofluorescence detection of MYHC demonstrated that miR-29b-1-5p can promote the differentiation of myoblasts. The functional enrichment analysis revealed that the target genes of miR-29b-1-5p may be involved in muscle tissue development, muscle organ development, and striated muscle tissue development, which are biological processes related to muscle development. The correlation analysis showed that these 6 genes, that is, ankyrin repeat domain 9 (ANKRD9), lactate dehydrogenase A (LDHA), transcription factor 12 (TCF12), FAT atypical cadherin 1 (FAT1), lin-9 homolog (LIN9), and integrin beta 3 binding protein (ITGB3BP), can be used as effective candidate target genes of miR-29b-1-5p. Moreover, miR-29b-1-5p inhibits the expression of ANKRD9 by directly binding the 3′UTR of ANKRD9. Overall, these data indicate that miR-29b-1-5p inhibits the proliferation of primary chicken myoblasts, stimulates their differentiation, and is involved in the process of muscle development and that its effective target gene is ANKRD9. This study identified the molecular mechanism of miR-29b-1-5p in chicken muscle development.
format article
author Yuanfang Li
Bin Zhai
Pengtao Yuan
Shengxin Fan
Wenjiao Jin
Wenting Li
Guirong Sun
Yadong Tian
Xiaojun Liu
Xiangtao Kang
Guoxi Li
author_facet Yuanfang Li
Bin Zhai
Pengtao Yuan
Shengxin Fan
Wenjiao Jin
Wenting Li
Guirong Sun
Yadong Tian
Xiaojun Liu
Xiangtao Kang
Guoxi Li
author_sort Yuanfang Li
title MiR-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets
title_short MiR-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets
title_full MiR-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets
title_fullStr MiR-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets
title_full_unstemmed MiR-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets
title_sort mir-29b-1-5p regulates the proliferation and differentiation of chicken primary myoblasts and analysis of its effective targets
publisher Elsevier
publishDate 2022
url https://doaj.org/article/30f150d5cf114bf598999a41728487c6
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