Linking the oral microbiome and salivary cytokine abundance to circadian oscillations

Abstract Saliva has immense potential as a diagnostic fluid for identification and monitoring of several systemic diseases. Composition of the microbiome and inflammation has been associated and reflective of oral and overall health. In addition, the relative ease of collection of saliva further str...

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Autores principales:	Anujit Sarkar, Melanie N. Kuehl, Amy C. Alman, Brant R. Burkhardt
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/30f45f65763945aa938639ea51350274
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spelling	oai:doaj.org-article:30f45f65763945aa938639ea513502742021-12-02T13:24:26ZLinking the oral microbiome and salivary cytokine abundance to circadian oscillations10.1038/s41598-021-81420-32045-2322https://doaj.org/article/30f45f65763945aa938639ea513502742021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81420-3https://doaj.org/toc/2045-2322Abstract Saliva has immense potential as a diagnostic fluid for identification and monitoring of several systemic diseases. Composition of the microbiome and inflammation has been associated and reflective of oral and overall health. In addition, the relative ease of collection of saliva further strengthens large-scale diagnostic purposes. However, the future clinical utility of saliva cannot be fully determined without a detailed examination of daily fluctuations that may occur within the oral microbiome and inflammation due to circadian rhythm. In this study, we explored the association between the salivary microbiome and the concentration of IL-1β, IL-6 and IL-8 in the saliva of 12 healthy adults over a period of 24 h by studying the 16S rRNA gene followed by negative binomial mixed model regression analysis. To determine the periodicity and oscillation patterns of both the oral microbiome and inflammation (represented by the cytokine levels), two of the twelve subjects were studied for three consecutive days. Our results indicate that the Operational Taxonomic Units (OTUs) belonging to Prevotella, SR1 and Ruminococcaceae are significantly associated to IL-1β while Prevotella and Granulicatella were associated with IL-8. Our findings have also revealed a periodicity of both the oral microbiome (OTUs) and inflammation (cytokine levels) with identifiable patterns between IL-1β and Prevotella, and IL-6 with Prevotella, Neisseria and Porphyromonas. We believe that this study represents the first measure and demonstration of simultaneous periodic fluctuations of cytokine levels and specific populations of the oral microbiome.Anujit SarkarMelanie N. KuehlAmy C. AlmanBrant R. BurkhardtNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anujit Sarkar Melanie N. Kuehl Amy C. Alman Brant R. Burkhardt Linking the oral microbiome and salivary cytokine abundance to circadian oscillations
description	Abstract Saliva has immense potential as a diagnostic fluid for identification and monitoring of several systemic diseases. Composition of the microbiome and inflammation has been associated and reflective of oral and overall health. In addition, the relative ease of collection of saliva further strengthens large-scale diagnostic purposes. However, the future clinical utility of saliva cannot be fully determined without a detailed examination of daily fluctuations that may occur within the oral microbiome and inflammation due to circadian rhythm. In this study, we explored the association between the salivary microbiome and the concentration of IL-1β, IL-6 and IL-8 in the saliva of 12 healthy adults over a period of 24 h by studying the 16S rRNA gene followed by negative binomial mixed model regression analysis. To determine the periodicity and oscillation patterns of both the oral microbiome and inflammation (represented by the cytokine levels), two of the twelve subjects were studied for three consecutive days. Our results indicate that the Operational Taxonomic Units (OTUs) belonging to Prevotella, SR1 and Ruminococcaceae are significantly associated to IL-1β while Prevotella and Granulicatella were associated with IL-8. Our findings have also revealed a periodicity of both the oral microbiome (OTUs) and inflammation (cytokine levels) with identifiable patterns between IL-1β and Prevotella, and IL-6 with Prevotella, Neisseria and Porphyromonas. We believe that this study represents the first measure and demonstration of simultaneous periodic fluctuations of cytokine levels and specific populations of the oral microbiome.
format	article
author	Anujit Sarkar Melanie N. Kuehl Amy C. Alman Brant R. Burkhardt
author_facet	Anujit Sarkar Melanie N. Kuehl Amy C. Alman Brant R. Burkhardt
author_sort	Anujit Sarkar
title	Linking the oral microbiome and salivary cytokine abundance to circadian oscillations
title_short	Linking the oral microbiome and salivary cytokine abundance to circadian oscillations
title_full	Linking the oral microbiome and salivary cytokine abundance to circadian oscillations
title_fullStr	Linking the oral microbiome and salivary cytokine abundance to circadian oscillations
title_full_unstemmed	Linking the oral microbiome and salivary cytokine abundance to circadian oscillations
title_sort	linking the oral microbiome and salivary cytokine abundance to circadian oscillations
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/30f45f65763945aa938639ea51350274
work_keys_str_mv	AT anujitsarkar linkingtheoralmicrobiomeandsalivarycytokineabundancetocircadianoscillations AT melanienkuehl linkingtheoralmicrobiomeandsalivarycytokineabundancetocircadianoscillations AT amycalman linkingtheoralmicrobiomeandsalivarycytokineabundancetocircadianoscillations AT brantrburkhardt linkingtheoralmicrobiomeandsalivarycytokineabundancetocircadianoscillations
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Linking the oral microbiome and salivary cytokine abundance to circadian oscillations

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