808-nm Photobiomodulation Affects the Viability of a Head and Neck Squamous Carcinoma Cellular Model, Acting on Energy Metabolism and Oxidative Stress Production

Photobiomodulation (PBM) is a form of low-dose light therapy that acts through energy delivery from non-ionizing sources. During the recent two decades, there has been tremendous progress with PBM acceptance in medicine. However, PBM effects on potential stimulation of existing malignant or pre-mali...

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Autores principales:	Silvia Ravera, Nadia Bertola, Claudio Pasquale, Silvia Bruno, Stefano Benedicenti, Sara Ferrando, Angelina Zekiy, Praveen Arany, Andrea Amaroli
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	apoptosis antioxidant defenses light therapy low-level laser therapy oral cancer oxidative phosphorylation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/30f789d67d3a45fb9c306f8e5c421c0b
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spelling	oai:doaj.org-article:30f789d67d3a45fb9c306f8e5c421c0b2021-11-25T16:51:15Z808-nm Photobiomodulation Affects the Viability of a Head and Neck Squamous Carcinoma Cellular Model, Acting on Energy Metabolism and Oxidative Stress Production10.3390/biomedicines91117172227-9059https://doaj.org/article/30f789d67d3a45fb9c306f8e5c421c0b2021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1717https://doaj.org/toc/2227-9059Photobiomodulation (PBM) is a form of low-dose light therapy that acts through energy delivery from non-ionizing sources. During the recent two decades, there has been tremendous progress with PBM acceptance in medicine. However, PBM effects on potential stimulation of existing malignant or pre-malignant cells remain unknown. Thus, the primary endpoint was to assess the safety of PBM treatment parameters on head and neck squamous cell carcinoma (HNSCC) proliferation or survival. The secondary endpoint was to assess any putative anti-cancer effects of PBM treatments. Cell viability, energy metabolism, oxidative stress, and pro- and anti-apoptotic markers expression were investigated on a Human Head and Neck Squamous Cell Carcinoma cellular model (OHSU-974 FAcorr cell line). PBM therapy was administered through the 810 nm diode laser (GaAlAs) device (Garda Laser, 7024 Negrar, Verona, Italy) at the powers of 0, 0.25, 0.50, 0.75, 1.00, or 1.25 W in continuous wave (CW) mode for an exposure time of 60 s with a spot-size of 1 cm<sup>2</sup> and with a distance of 1.86 cm from the cells. Results showed that 810-nm PBM affected oxidative phosphorylation in OHSU-971 FAcorr, causing a metabolic switch to anaerobic glycolysis. In addition, PBM reduced the catalase activity, determining an unbalance between oxidative stress production and the antioxidant defenses, which could stimulate the pro-apoptotic cellular pathways. Our data, at the parameters investigated, suggest the safeness of PBM as a supportive cancer therapy. Pre-clinical and clinical studies are necessary to confirm the in vitro evidence.Silvia RaveraNadia BertolaClaudio PasqualeSilvia BrunoStefano BenedicentiSara FerrandoAngelina ZekiyPraveen AranyAndrea AmaroliMDPI AGarticleapoptosisantioxidant defenseslight therapylow-level laser therapyoral canceroxidative phosphorylationBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1717, p 1717 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	apoptosis antioxidant defenses light therapy low-level laser therapy oral cancer oxidative phosphorylation Biology (General) QH301-705.5
spellingShingle	apoptosis antioxidant defenses light therapy low-level laser therapy oral cancer oxidative phosphorylation Biology (General) QH301-705.5 Silvia Ravera Nadia Bertola Claudio Pasquale Silvia Bruno Stefano Benedicenti Sara Ferrando Angelina Zekiy Praveen Arany Andrea Amaroli 808-nm Photobiomodulation Affects the Viability of a Head and Neck Squamous Carcinoma Cellular Model, Acting on Energy Metabolism and Oxidative Stress Production
description	Photobiomodulation (PBM) is a form of low-dose light therapy that acts through energy delivery from non-ionizing sources. During the recent two decades, there has been tremendous progress with PBM acceptance in medicine. However, PBM effects on potential stimulation of existing malignant or pre-malignant cells remain unknown. Thus, the primary endpoint was to assess the safety of PBM treatment parameters on head and neck squamous cell carcinoma (HNSCC) proliferation or survival. The secondary endpoint was to assess any putative anti-cancer effects of PBM treatments. Cell viability, energy metabolism, oxidative stress, and pro- and anti-apoptotic markers expression were investigated on a Human Head and Neck Squamous Cell Carcinoma cellular model (OHSU-974 FAcorr cell line). PBM therapy was administered through the 810 nm diode laser (GaAlAs) device (Garda Laser, 7024 Negrar, Verona, Italy) at the powers of 0, 0.25, 0.50, 0.75, 1.00, or 1.25 W in continuous wave (CW) mode for an exposure time of 60 s with a spot-size of 1 cm<sup>2</sup> and with a distance of 1.86 cm from the cells. Results showed that 810-nm PBM affected oxidative phosphorylation in OHSU-971 FAcorr, causing a metabolic switch to anaerobic glycolysis. In addition, PBM reduced the catalase activity, determining an unbalance between oxidative stress production and the antioxidant defenses, which could stimulate the pro-apoptotic cellular pathways. Our data, at the parameters investigated, suggest the safeness of PBM as a supportive cancer therapy. Pre-clinical and clinical studies are necessary to confirm the in vitro evidence.
format	article
author	Silvia Ravera Nadia Bertola Claudio Pasquale Silvia Bruno Stefano Benedicenti Sara Ferrando Angelina Zekiy Praveen Arany Andrea Amaroli
author_facet	Silvia Ravera Nadia Bertola Claudio Pasquale Silvia Bruno Stefano Benedicenti Sara Ferrando Angelina Zekiy Praveen Arany Andrea Amaroli
author_sort	Silvia Ravera
title	808-nm Photobiomodulation Affects the Viability of a Head and Neck Squamous Carcinoma Cellular Model, Acting on Energy Metabolism and Oxidative Stress Production
title_short	808-nm Photobiomodulation Affects the Viability of a Head and Neck Squamous Carcinoma Cellular Model, Acting on Energy Metabolism and Oxidative Stress Production
title_full	808-nm Photobiomodulation Affects the Viability of a Head and Neck Squamous Carcinoma Cellular Model, Acting on Energy Metabolism and Oxidative Stress Production
title_fullStr	808-nm Photobiomodulation Affects the Viability of a Head and Neck Squamous Carcinoma Cellular Model, Acting on Energy Metabolism and Oxidative Stress Production
title_full_unstemmed	808-nm Photobiomodulation Affects the Viability of a Head and Neck Squamous Carcinoma Cellular Model, Acting on Energy Metabolism and Oxidative Stress Production
title_sort	808-nm photobiomodulation affects the viability of a head and neck squamous carcinoma cellular model, acting on energy metabolism and oxidative stress production
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/30f789d67d3a45fb9c306f8e5c421c0b
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808-nm Photobiomodulation Affects the Viability of a Head and Neck Squamous Carcinoma Cellular Model, Acting on Energy Metabolism and Oxidative Stress Production

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