Morphological Dynamics of Leukemia Cells on TiO2 Nanoparticle Coatings Studied by AFM

Coatings of modified TiO2 nanoparticles (TiO2-m) have been shown to effectively and selectively trap non-adherent cancer cells, with an enormous potential for applications in photodynamic therapy (PDT). Leukemia cells have a remarkable affinity for TiO&l...

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Autores principales:	Jaime Andres Garcia Diosa, Alejandro Gonzalez Orive, Guido Grundmeier, Ruben Jesus Camargo Amado, Adrian Keller
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cancer cells leukemia nanoparticle coatings cell adhesion TiO<sub>2</sub> Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/30f815b20638481e9f9b984b856e6f86
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spelling	oai:doaj.org-article:30f815b20638481e9f9b984b856e6f862021-11-11T15:00:09ZMorphological Dynamics of Leukemia Cells on TiO<sub>2</sub> Nanoparticle Coatings Studied by AFM10.3390/app112198982076-3417https://doaj.org/article/30f815b20638481e9f9b984b856e6f862021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/21/9898https://doaj.org/toc/2076-3417Coatings of modified TiO<sub>2</sub> nanoparticles (TiO<sub>2</sub>-m) have been shown to effectively and selectively trap non-adherent cancer cells, with an enormous potential for applications in photodynamic therapy (PDT). Leukemia cells have a remarkable affinity for TiO<sub>2</sub>-m coatings, adhering to the surface by membrane structures and exhibiting morphologic characteristics of amoeboid locomotion. However, the details of the cell–substrate interaction induced by the TiO<sub>2</sub>-m coating remain elusive. With the aim to obtain a better understanding of this phenomenon, leukemia cell adhesion to such coatings was characterized by atomic force microscopy (AFM) for short contact times up to 60 min. The cell and membrane morphological parameters mean cell height, contact area, cell volume, and membrane roughness were determined at different contact times. These results reveal cell expansion and contraction phases occurring during the initial stage of adhesion. Subsequently, the leukemic cells reach what appears to be a new resting state, characterized by pinning of the cell membrane by TiO<sub>2</sub>-m nanoparticle aggregates protruding from the coating surface.Jaime Andres Garcia DiosaAlejandro Gonzalez OriveGuido GrundmeierRuben Jesus Camargo AmadoAdrian KellerMDPI AGarticlecancer cellsleukemiananoparticle coatingscell adhesionTiO<sub>2</sub>TechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 9898, p 9898 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cancer cells leukemia nanoparticle coatings cell adhesion TiO<sub>2</sub> Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	cancer cells leukemia nanoparticle coatings cell adhesion TiO<sub>2</sub> Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Jaime Andres Garcia Diosa Alejandro Gonzalez Orive Guido Grundmeier Ruben Jesus Camargo Amado Adrian Keller Morphological Dynamics of Leukemia Cells on TiO<sub>2</sub> Nanoparticle Coatings Studied by AFM
description	Coatings of modified TiO<sub>2</sub> nanoparticles (TiO<sub>2</sub>-m) have been shown to effectively and selectively trap non-adherent cancer cells, with an enormous potential for applications in photodynamic therapy (PDT). Leukemia cells have a remarkable affinity for TiO<sub>2</sub>-m coatings, adhering to the surface by membrane structures and exhibiting morphologic characteristics of amoeboid locomotion. However, the details of the cell–substrate interaction induced by the TiO<sub>2</sub>-m coating remain elusive. With the aim to obtain a better understanding of this phenomenon, leukemia cell adhesion to such coatings was characterized by atomic force microscopy (AFM) for short contact times up to 60 min. The cell and membrane morphological parameters mean cell height, contact area, cell volume, and membrane roughness were determined at different contact times. These results reveal cell expansion and contraction phases occurring during the initial stage of adhesion. Subsequently, the leukemic cells reach what appears to be a new resting state, characterized by pinning of the cell membrane by TiO<sub>2</sub>-m nanoparticle aggregates protruding from the coating surface.
format	article
author	Jaime Andres Garcia Diosa Alejandro Gonzalez Orive Guido Grundmeier Ruben Jesus Camargo Amado Adrian Keller
author_facet	Jaime Andres Garcia Diosa Alejandro Gonzalez Orive Guido Grundmeier Ruben Jesus Camargo Amado Adrian Keller
author_sort	Jaime Andres Garcia Diosa
title	Morphological Dynamics of Leukemia Cells on TiO<sub>2</sub> Nanoparticle Coatings Studied by AFM
title_short	Morphological Dynamics of Leukemia Cells on TiO<sub>2</sub> Nanoparticle Coatings Studied by AFM
title_full	Morphological Dynamics of Leukemia Cells on TiO<sub>2</sub> Nanoparticle Coatings Studied by AFM
title_fullStr	Morphological Dynamics of Leukemia Cells on TiO<sub>2</sub> Nanoparticle Coatings Studied by AFM
title_full_unstemmed	Morphological Dynamics of Leukemia Cells on TiO<sub>2</sub> Nanoparticle Coatings Studied by AFM
title_sort	morphological dynamics of leukemia cells on tio<sub>2</sub> nanoparticle coatings studied by afm
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/30f815b20638481e9f9b984b856e6f86
work_keys_str_mv	AT jaimeandresgarciadiosa morphologicaldynamicsofleukemiacellsontiosub2subnanoparticlecoatingsstudiedbyafm AT alejandrogonzalezorive morphologicaldynamicsofleukemiacellsontiosub2subnanoparticlecoatingsstudiedbyafm AT guidogrundmeier morphologicaldynamicsofleukemiacellsontiosub2subnanoparticlecoatingsstudiedbyafm AT rubenjesuscamargoamado morphologicaldynamicsofleukemiacellsontiosub2subnanoparticlecoatingsstudiedbyafm AT adriankeller morphologicaldynamicsofleukemiacellsontiosub2subnanoparticlecoatingsstudiedbyafm
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Morphological Dynamics of Leukemia Cells on TiO<sub>2</sub> Nanoparticle Coatings Studied by AFM

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