Preparation, characterization and in-vitro evaluation of sustained release protein-loaded nanoparticles based on biodegradable polymers

Biswajit Mukherjee, Kousik Santra, Gurudutta Pattnaik, Soma GhoshDepartment of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, IndiaAbstract: Controlled drug delivery technology of proteins/peptides from biodegradable nanoparticles has emerged as one of the eminent areas to ove...

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Autores principales:	Biswajit Mukherjee, Kousik Santra, Gurudutta Pattnaik, Soma Ghosh
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2008
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/30ffa614628543e1a74d89686d9d0876
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spelling	oai:doaj.org-article:30ffa614628543e1a74d89686d9d08762021-12-02T08:08:30ZPreparation, characterization and in-vitro evaluation of sustained release protein-loaded nanoparticles based on biodegradable polymers1176-91141178-2013https://doaj.org/article/30ffa614628543e1a74d89686d9d08762008-10-01T00:00:00Zhttp://www.dovepress.com/preparation-characterization-and-in-vitro-evaluation-of-sustained-rele-a2471https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Biswajit Mukherjee, Kousik Santra, Gurudutta Pattnaik, Soma GhoshDepartment of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, IndiaAbstract: Controlled drug delivery technology of proteins/peptides from biodegradable nanoparticles has emerged as one of the eminent areas to overcome formulation associated problems of the macromolecules. The purpose of the present investigation was to develop protein-loaded nanoparticles using biodegradable polymer poly L-lactide-co-glycolidic acid (PLGA) with bovine serum albumin (BSA) as a model protein. Despite many studies available with PLGA-based protein-loaded nanoparticles, production know-how, process parameters, protein loading, duration of protein release, narrowing polydispersity of particles have not been investigated enough to scale up manufacturing of protein-loaded nanoparticles in formulations. Different process parameters such as protein/polymer ratio, homogenizing speed during emulsifications, particle surface morphology and surface charges, particle size analysis and in-vitro protein release were investigated. The in-vitro protein release study suggests that release profile of BSA from nanoparticles could be modulated by changing protein-polymer ratios and/or by varying homogenizing speed during multiple-emulsion preparation technique. The formulation prepared with protein-polymer ratio of 1:60 at 17,500 rpm gave maximum protein-loading, minimum polydispersion with maximally sustained protein release pattern, among the prepared formulations. Decreased (10,000 rpm) or enhanced (24,000 rpm) homogenizing speeds resulted in increased polydispersion with larger particles having no better protein-loading and -release profiles in the present study.Keywords: bovine serum albumin (BSA), poly-L-lactide-co-glycolidic acid (PLGA), nanoparticles Biswajit MukherjeeKousik SantraGurudutta PattnaikSoma GhoshDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2008, Iss Issue 4, Pp 487-496 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Biswajit Mukherjee Kousik Santra Gurudutta Pattnaik Soma Ghosh Preparation, characterization and in-vitro evaluation of sustained release protein-loaded nanoparticles based on biodegradable polymers
description	Biswajit Mukherjee, Kousik Santra, Gurudutta Pattnaik, Soma GhoshDepartment of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, IndiaAbstract: Controlled drug delivery technology of proteins/peptides from biodegradable nanoparticles has emerged as one of the eminent areas to overcome formulation associated problems of the macromolecules. The purpose of the present investigation was to develop protein-loaded nanoparticles using biodegradable polymer poly L-lactide-co-glycolidic acid (PLGA) with bovine serum albumin (BSA) as a model protein. Despite many studies available with PLGA-based protein-loaded nanoparticles, production know-how, process parameters, protein loading, duration of protein release, narrowing polydispersity of particles have not been investigated enough to scale up manufacturing of protein-loaded nanoparticles in formulations. Different process parameters such as protein/polymer ratio, homogenizing speed during emulsifications, particle surface morphology and surface charges, particle size analysis and in-vitro protein release were investigated. The in-vitro protein release study suggests that release profile of BSA from nanoparticles could be modulated by changing protein-polymer ratios and/or by varying homogenizing speed during multiple-emulsion preparation technique. The formulation prepared with protein-polymer ratio of 1:60 at 17,500 rpm gave maximum protein-loading, minimum polydispersion with maximally sustained protein release pattern, among the prepared formulations. Decreased (10,000 rpm) or enhanced (24,000 rpm) homogenizing speeds resulted in increased polydispersion with larger particles having no better protein-loading and -release profiles in the present study.Keywords: bovine serum albumin (BSA), poly-L-lactide-co-glycolidic acid (PLGA), nanoparticles
format	article
author	Biswajit Mukherjee Kousik Santra Gurudutta Pattnaik Soma Ghosh
author_facet	Biswajit Mukherjee Kousik Santra Gurudutta Pattnaik Soma Ghosh
author_sort	Biswajit Mukherjee
title	Preparation, characterization and in-vitro evaluation of sustained release protein-loaded nanoparticles based on biodegradable polymers
title_short	Preparation, characterization and in-vitro evaluation of sustained release protein-loaded nanoparticles based on biodegradable polymers
title_full	Preparation, characterization and in-vitro evaluation of sustained release protein-loaded nanoparticles based on biodegradable polymers
title_fullStr	Preparation, characterization and in-vitro evaluation of sustained release protein-loaded nanoparticles based on biodegradable polymers
title_full_unstemmed	Preparation, characterization and in-vitro evaluation of sustained release protein-loaded nanoparticles based on biodegradable polymers
title_sort	preparation, characterization and in-vitro evaluation of sustained release protein-loaded nanoparticles based on biodegradable polymers
publisher	Dove Medical Press
publishDate	2008
url	https://doaj.org/article/30ffa614628543e1a74d89686d9d0876
work_keys_str_mv	AT biswajitmukherjee preparationcharacterizationandinvitroevaluationofsustainedreleaseproteinloadednanoparticlesbasedonbiodegradablepolymers AT kousiksantra preparationcharacterizationandinvitroevaluationofsustainedreleaseproteinloadednanoparticlesbasedonbiodegradablepolymers AT guruduttapattnaik preparationcharacterizationandinvitroevaluationofsustainedreleaseproteinloadednanoparticlesbasedonbiodegradablepolymers AT somaghosh preparationcharacterizationandinvitroevaluationofsustainedreleaseproteinloadednanoparticlesbasedonbiodegradablepolymers
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Preparation, characterization and in-vitro evaluation of sustained release protein-loaded nanoparticles based on biodegradable polymers

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