Two-stage clustering (TSC): a pipeline for selecting operational taxonomic units for the high-throughput sequencing of PCR amplicons.

Clustering 16S/18S rRNA amplicon sequences into operational taxonomic units (OTUs) is a critical step for the bioinformatic analysis of microbial diversity. Here, we report a pipeline for selecting OTUs with a relatively low computational demand and a high degree of accuracy. This pipeline is referr...

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Autores principales: Xiao-Tao Jiang, Hai Zhang, Hua-Fang Sheng, Yu Wang, Yan He, Fei Zou, Hong-Wei Zhou
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2012
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Acceso en línea:https://doaj.org/article/310758fd66834024b6fa766db45ac085
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