Growth Performance, Growth-Related Genes, Digestibility, Digestive Enzyme Activity, Immune and Stress Responses of <i>de novo</i> Camelina Meal in Diets of Red Seabream (<i>Pagrus major</i>)

A 60-day experiment was designed to assess the effect of different ratios of fish meal (FM): camelina meal plant protein (CM) on growth response and relative gene expression of growth-promoting factors, feed utilization potency, digestive enzymes activities, apparent digestibility (ADC), stress resp...

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Autores principales:	Kumbukani Mzengereza, Manabu Ishikawa, Shunsuke Koshio, Saichiro Yokoyama, Zhang Yukun, Ronick S. Shadrack, Seok Seo, Tomonari Kotani, Serge Dossou, Mohammed F. El Basuini, Mahmoud A. O. Dawood
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	plant protein meal red seabream fish meal health status growth-promoting genes Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/310cafe84dc14377961888c708a6e9a8
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spelling	oai:doaj.org-article:310cafe84dc14377961888c708a6e9a82021-11-25T16:15:59ZGrowth Performance, Growth-Related Genes, Digestibility, Digestive Enzyme Activity, Immune and Stress Responses of <i>de novo</i> Camelina Meal in Diets of Red Seabream (<i>Pagrus major</i>)10.3390/ani111131182076-2615https://doaj.org/article/310cafe84dc14377961888c708a6e9a82021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3118https://doaj.org/toc/2076-2615A 60-day experiment was designed to assess the effect of different ratios of fish meal (FM): camelina meal plant protein (CM) on growth response and relative gene expression of growth-promoting factors, feed utilization potency, digestive enzymes activities, apparent digestibility (ADC), stress response, non-specific immunity of <i>Pagrus major</i>. Four isonitrogenous (490.7 g/kg of crude protein) and isolipidic (91.5 g/kg total lipid) experimental diets were formulated and designated as camelina meal (CM0), soyabean meal (SBM20.5), CM20.5, and CM33 based on protein contents. At the end of the feed trial, significantly higher (<i>p</i> < 0.05) weight gain, specific growth rate, and feed intake but lower feed conversion ratio were recorded in fish fed CM0, SBM20.5, and CM20.5 than fish fed CM33. The lowest growth, feed utilization, enzyme activity, and digestibility were recorded in fish fed CM33. Significantly higher pepsin, amylase, and protease activities were observed in fish fed CM0, SBM20.5, and CM20.5 diets than fish fed CM33. The highest ADC of protein was recorded in fish fed CM0, SBM20.5, and CM20.5 diets. Hematocrit levels were depressed CM33 while total serum protein, total cholesterol, triglyceride, blood urea nitrogen, total bilirubin, aspartate aminotransferase, and alanine aminotransferase were not significantly changed by the inclusion of CM. Non-specific immune variables (lysozyme activity, peroxidase activity in serum and nitro blue tetrazolium) in fish fed CM0, SBM20.5, and CM20.5 were significantly higher than in fish fed CM33 diet. The superoxide dismutase of fish fed CM20.5 was not significantly different from CM0 and SBM20.5 (<i>p</i> > 0.05). Catalase and low salinity stress test show that CM0, SBM20.5, and CM20.5 were not significantly (<i>p</i> > 0.05) different, while CM33 was significantly lower than the rest of the diets. TBARs show that CM20.5 and CM33 diets were significantly different (<i>p</i> < 0.05), but CM20.5 was not significantly different from SBM20.5. Significantly higher hepatic <i>IGF-1</i> and <i>IGF-2</i> mRNA expression was found in fish-fed diet groups CM0, SBM20.5, and CM20.5 than fish fed CM33. The present study indicated that the addition of CM up 205 kg/kg to diet maintains growth, digestive enzymes, nutrient digestibility, immunity, stress resistance, and feed utilization efficiency of red sea bream.Kumbukani MzengerezaManabu IshikawaShunsuke KoshioSaichiro YokoyamaZhang YukunRonick S. ShadrackSeok SeoTomonari KotaniSerge DossouMohammed F. El BasuiniMahmoud A. O. DawoodMDPI AGarticleplant protein mealred seabreamfish mealhealth statusgrowth-promoting genesVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3118, p 3118 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	plant protein meal red seabream fish meal health status growth-promoting genes Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	plant protein meal red seabream fish meal health status growth-promoting genes Veterinary medicine SF600-1100 Zoology QL1-991 Kumbukani Mzengereza Manabu Ishikawa Shunsuke Koshio Saichiro Yokoyama Zhang Yukun Ronick S. Shadrack Seok Seo Tomonari Kotani Serge Dossou Mohammed F. El Basuini Mahmoud A. O. Dawood Growth Performance, Growth-Related Genes, Digestibility, Digestive Enzyme Activity, Immune and Stress Responses of <i>de novo</i> Camelina Meal in Diets of Red Seabream (<i>Pagrus major</i>)
description	A 60-day experiment was designed to assess the effect of different ratios of fish meal (FM): camelina meal plant protein (CM) on growth response and relative gene expression of growth-promoting factors, feed utilization potency, digestive enzymes activities, apparent digestibility (ADC), stress response, non-specific immunity of <i>Pagrus major</i>. Four isonitrogenous (490.7 g/kg of crude protein) and isolipidic (91.5 g/kg total lipid) experimental diets were formulated and designated as camelina meal (CM0), soyabean meal (SBM20.5), CM20.5, and CM33 based on protein contents. At the end of the feed trial, significantly higher (<i>p</i> < 0.05) weight gain, specific growth rate, and feed intake but lower feed conversion ratio were recorded in fish fed CM0, SBM20.5, and CM20.5 than fish fed CM33. The lowest growth, feed utilization, enzyme activity, and digestibility were recorded in fish fed CM33. Significantly higher pepsin, amylase, and protease activities were observed in fish fed CM0, SBM20.5, and CM20.5 diets than fish fed CM33. The highest ADC of protein was recorded in fish fed CM0, SBM20.5, and CM20.5 diets. Hematocrit levels were depressed CM33 while total serum protein, total cholesterol, triglyceride, blood urea nitrogen, total bilirubin, aspartate aminotransferase, and alanine aminotransferase were not significantly changed by the inclusion of CM. Non-specific immune variables (lysozyme activity, peroxidase activity in serum and nitro blue tetrazolium) in fish fed CM0, SBM20.5, and CM20.5 were significantly higher than in fish fed CM33 diet. The superoxide dismutase of fish fed CM20.5 was not significantly different from CM0 and SBM20.5 (<i>p</i> > 0.05). Catalase and low salinity stress test show that CM0, SBM20.5, and CM20.5 were not significantly (<i>p</i> > 0.05) different, while CM33 was significantly lower than the rest of the diets. TBARs show that CM20.5 and CM33 diets were significantly different (<i>p</i> < 0.05), but CM20.5 was not significantly different from SBM20.5. Significantly higher hepatic <i>IGF-1</i> and <i>IGF-2</i> mRNA expression was found in fish-fed diet groups CM0, SBM20.5, and CM20.5 than fish fed CM33. The present study indicated that the addition of CM up 205 kg/kg to diet maintains growth, digestive enzymes, nutrient digestibility, immunity, stress resistance, and feed utilization efficiency of red sea bream.
format	article
author	Kumbukani Mzengereza Manabu Ishikawa Shunsuke Koshio Saichiro Yokoyama Zhang Yukun Ronick S. Shadrack Seok Seo Tomonari Kotani Serge Dossou Mohammed F. El Basuini Mahmoud A. O. Dawood
author_facet	Kumbukani Mzengereza Manabu Ishikawa Shunsuke Koshio Saichiro Yokoyama Zhang Yukun Ronick S. Shadrack Seok Seo Tomonari Kotani Serge Dossou Mohammed F. El Basuini Mahmoud A. O. Dawood
author_sort	Kumbukani Mzengereza
title	Growth Performance, Growth-Related Genes, Digestibility, Digestive Enzyme Activity, Immune and Stress Responses of <i>de novo</i> Camelina Meal in Diets of Red Seabream (<i>Pagrus major</i>)
title_short	Growth Performance, Growth-Related Genes, Digestibility, Digestive Enzyme Activity, Immune and Stress Responses of <i>de novo</i> Camelina Meal in Diets of Red Seabream (<i>Pagrus major</i>)
title_full	Growth Performance, Growth-Related Genes, Digestibility, Digestive Enzyme Activity, Immune and Stress Responses of <i>de novo</i> Camelina Meal in Diets of Red Seabream (<i>Pagrus major</i>)
title_fullStr	Growth Performance, Growth-Related Genes, Digestibility, Digestive Enzyme Activity, Immune and Stress Responses of <i>de novo</i> Camelina Meal in Diets of Red Seabream (<i>Pagrus major</i>)
title_full_unstemmed	Growth Performance, Growth-Related Genes, Digestibility, Digestive Enzyme Activity, Immune and Stress Responses of <i>de novo</i> Camelina Meal in Diets of Red Seabream (<i>Pagrus major</i>)
title_sort	growth performance, growth-related genes, digestibility, digestive enzyme activity, immune and stress responses of <i>de novo</i> camelina meal in diets of red seabream (<i>pagrus major</i>)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/310cafe84dc14377961888c708a6e9a8
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Growth Performance, Growth-Related Genes, Digestibility, Digestive Enzyme Activity, Immune and Stress Responses of <i>de novo</i> Camelina Meal in Diets of Red Seabream (<i>Pagrus major</i>)

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