Biodegradation of a Complex Phenolic Industrial Stream by Bacterial Strains Isolated from Industrial Wastewaters

Molecular and metabolomic tools were used to design and understand the biodegradation of phenolic compounds in real industrial streams. Bacterial species were isolated from an industrial wastewater treatment plant of a phenol production factory and identified using molecular techniques. Next, the bi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alejandra Bartolomé, Gema Rodríguez-Moro, Juan-Luis Fuentes, Mariana Lopes, Juana Frontela, Jesús Lázaro, María Cuaresma, José-Luis Gómez-Ariza, Tamara García-Barrera, Carlos Vílchez
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	phenolic compounds biodegradation <i>Pseudomonas</i> <i>Alcaligenes</i> massive sequencing metabolomics Chemical technology TP1-1185 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/310d6dcd284a40c2843179fe29de1c19
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:310d6dcd284a40c2843179fe29de1c19
record_format	dspace
spelling	oai:doaj.org-article:310d6dcd284a40c2843179fe29de1c192021-11-25T18:50:53ZBiodegradation of a Complex Phenolic Industrial Stream by Bacterial Strains Isolated from Industrial Wastewaters10.3390/pr91119642227-9717https://doaj.org/article/310d6dcd284a40c2843179fe29de1c192021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9717/9/11/1964https://doaj.org/toc/2227-9717Molecular and metabolomic tools were used to design and understand the biodegradation of phenolic compounds in real industrial streams. Bacterial species were isolated from an industrial wastewater treatment plant of a phenol production factory and identified using molecular techniques. Next, the biodegradation potential of the most promising strains was analyzed in the presence of a phenolic industrial by-product containing phenol, alfa-methylstyrene, acetophenone, 2-cumylphenol, and 4-cumylphenol. A bacterial consortium comprising <i>Pseudomonas</i> and <i>Alcaligenes</i> species was assessed for its ability to degrade phenolic compounds from the phenolic industrial stream (PS). The consortium adapted itself to the increasing levels of phenolic compounds, roughly up to 1750 ppm of PS; thus, becoming resistant to them. In addition, the consortium exhibited the ability to grow in the presence of PS in repeated batch mode processes. Results from untargeted metabolomic analysis of the culture medium in the presence of PS suggested that bacteria transformed the toxic phenolic compounds into less harmful molecules as a survival mechanism. Overall, the study demonstrates the usefulness of massive sequencing and metabolomic tools in constructing bacterial consortia that can efficiently biodegrade complex PS. Furthermore, it improves our understanding of their biodegradation capabilities.Alejandra BartoloméGema Rodríguez-MoroJuan-Luis FuentesMariana LopesJuana FrontelaJesús LázaroMaría CuaresmaJosé-Luis Gómez-ArizaTamara García-BarreraCarlos VílchezMDPI AGarticlephenolic compoundsbiodegradation<i>Pseudomonas</i><i>Alcaligenes</i>massive sequencingmetabolomicsChemical technologyTP1-1185ChemistryQD1-999ENProcesses, Vol 9, Iss 1964, p 1964 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	phenolic compounds biodegradation <i>Pseudomonas</i> <i>Alcaligenes</i> massive sequencing metabolomics Chemical technology TP1-1185 Chemistry QD1-999
spellingShingle	phenolic compounds biodegradation <i>Pseudomonas</i> <i>Alcaligenes</i> massive sequencing metabolomics Chemical technology TP1-1185 Chemistry QD1-999 Alejandra Bartolomé Gema Rodríguez-Moro Juan-Luis Fuentes Mariana Lopes Juana Frontela Jesús Lázaro María Cuaresma José-Luis Gómez-Ariza Tamara García-Barrera Carlos Vílchez Biodegradation of a Complex Phenolic Industrial Stream by Bacterial Strains Isolated from Industrial Wastewaters
description	Molecular and metabolomic tools were used to design and understand the biodegradation of phenolic compounds in real industrial streams. Bacterial species were isolated from an industrial wastewater treatment plant of a phenol production factory and identified using molecular techniques. Next, the biodegradation potential of the most promising strains was analyzed in the presence of a phenolic industrial by-product containing phenol, alfa-methylstyrene, acetophenone, 2-cumylphenol, and 4-cumylphenol. A bacterial consortium comprising <i>Pseudomonas</i> and <i>Alcaligenes</i> species was assessed for its ability to degrade phenolic compounds from the phenolic industrial stream (PS). The consortium adapted itself to the increasing levels of phenolic compounds, roughly up to 1750 ppm of PS; thus, becoming resistant to them. In addition, the consortium exhibited the ability to grow in the presence of PS in repeated batch mode processes. Results from untargeted metabolomic analysis of the culture medium in the presence of PS suggested that bacteria transformed the toxic phenolic compounds into less harmful molecules as a survival mechanism. Overall, the study demonstrates the usefulness of massive sequencing and metabolomic tools in constructing bacterial consortia that can efficiently biodegrade complex PS. Furthermore, it improves our understanding of their biodegradation capabilities.
format	article
author	Alejandra Bartolomé Gema Rodríguez-Moro Juan-Luis Fuentes Mariana Lopes Juana Frontela Jesús Lázaro María Cuaresma José-Luis Gómez-Ariza Tamara García-Barrera Carlos Vílchez
author_facet	Alejandra Bartolomé Gema Rodríguez-Moro Juan-Luis Fuentes Mariana Lopes Juana Frontela Jesús Lázaro María Cuaresma José-Luis Gómez-Ariza Tamara García-Barrera Carlos Vílchez
author_sort	Alejandra Bartolomé
title	Biodegradation of a Complex Phenolic Industrial Stream by Bacterial Strains Isolated from Industrial Wastewaters
title_short	Biodegradation of a Complex Phenolic Industrial Stream by Bacterial Strains Isolated from Industrial Wastewaters
title_full	Biodegradation of a Complex Phenolic Industrial Stream by Bacterial Strains Isolated from Industrial Wastewaters
title_fullStr	Biodegradation of a Complex Phenolic Industrial Stream by Bacterial Strains Isolated from Industrial Wastewaters
title_full_unstemmed	Biodegradation of a Complex Phenolic Industrial Stream by Bacterial Strains Isolated from Industrial Wastewaters
title_sort	biodegradation of a complex phenolic industrial stream by bacterial strains isolated from industrial wastewaters
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/310d6dcd284a40c2843179fe29de1c19
work_keys_str_mv	AT alejandrabartolome biodegradationofacomplexphenolicindustrialstreambybacterialstrainsisolatedfromindustrialwastewaters AT gemarodriguezmoro biodegradationofacomplexphenolicindustrialstreambybacterialstrainsisolatedfromindustrialwastewaters AT juanluisfuentes biodegradationofacomplexphenolicindustrialstreambybacterialstrainsisolatedfromindustrialwastewaters AT marianalopes biodegradationofacomplexphenolicindustrialstreambybacterialstrainsisolatedfromindustrialwastewaters AT juanafrontela biodegradationofacomplexphenolicindustrialstreambybacterialstrainsisolatedfromindustrialwastewaters AT jesuslazaro biodegradationofacomplexphenolicindustrialstreambybacterialstrainsisolatedfromindustrialwastewaters AT mariacuaresma biodegradationofacomplexphenolicindustrialstreambybacterialstrainsisolatedfromindustrialwastewaters AT joseluisgomezariza biodegradationofacomplexphenolicindustrialstreambybacterialstrainsisolatedfromindustrialwastewaters AT tamaragarciabarrera biodegradationofacomplexphenolicindustrialstreambybacterialstrainsisolatedfromindustrialwastewaters AT carlosvilchez biodegradationofacomplexphenolicindustrialstreambybacterialstrainsisolatedfromindustrialwastewaters
_version_	1718410639804727296

Biodegradation of a Complex Phenolic Industrial Stream by Bacterial Strains Isolated from Industrial Wastewaters

Ejemplares similares