ProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization

Abstract Fluorescence in situ hybridization (FISH) is a powerful method to visualize the spatial positions of specific genomic loci and RNA species. Recent technological advances have leveraged FISH to visualize these features in a highly multiplexed manner. Notable examples include chromatin tracin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mengwei Hu, Bing Yang, Yubao Cheng, Jonathan S. D. Radda, Yanbo Chen, Miao Liu, Siyuan Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/31111c7780434b0eb86d2f873fb1a7f4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:31111c7780434b0eb86d2f873fb1a7f4
record_format	dspace
spelling	oai:doaj.org-article:31111c7780434b0eb86d2f873fb1a7f42021-12-02T13:33:59ZProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization10.1038/s41598-020-76439-x2045-2322https://doaj.org/article/31111c7780434b0eb86d2f873fb1a7f42020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-76439-xhttps://doaj.org/toc/2045-2322Abstract Fluorescence in situ hybridization (FISH) is a powerful method to visualize the spatial positions of specific genomic loci and RNA species. Recent technological advances have leveraged FISH to visualize these features in a highly multiplexed manner. Notable examples include chromatin tracing, RNA multiplexed error-robust FISH (MERFISH), multiplexed imaging of nucleome architectures (MINA), and sequential single-molecule RNA FISH. However, one obstacle to the broad adoption of these methods is the complexity of the multiplexed FISH probe design. In this paper, we introduce an easy-to-use, versatile, and all-in-one application called ProbeDealer to design probes for a variety of multiplexed FISH techniques and their combinations. ProbeDealer offers a one-stop shop for multiplexed FISH design needs of the research community.Mengwei HuBing YangYubao ChengJonathan S. D. RaddaYanbo ChenMiao LiuSiyuan WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-7 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mengwei Hu Bing Yang Yubao Cheng Jonathan S. D. Radda Yanbo Chen Miao Liu Siyuan Wang ProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization
description	Abstract Fluorescence in situ hybridization (FISH) is a powerful method to visualize the spatial positions of specific genomic loci and RNA species. Recent technological advances have leveraged FISH to visualize these features in a highly multiplexed manner. Notable examples include chromatin tracing, RNA multiplexed error-robust FISH (MERFISH), multiplexed imaging of nucleome architectures (MINA), and sequential single-molecule RNA FISH. However, one obstacle to the broad adoption of these methods is the complexity of the multiplexed FISH probe design. In this paper, we introduce an easy-to-use, versatile, and all-in-one application called ProbeDealer to design probes for a variety of multiplexed FISH techniques and their combinations. ProbeDealer offers a one-stop shop for multiplexed FISH design needs of the research community.
format	article
author	Mengwei Hu Bing Yang Yubao Cheng Jonathan S. D. Radda Yanbo Chen Miao Liu Siyuan Wang
author_facet	Mengwei Hu Bing Yang Yubao Cheng Jonathan S. D. Radda Yanbo Chen Miao Liu Siyuan Wang
author_sort	Mengwei Hu
title	ProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization
title_short	ProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization
title_full	ProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization
title_fullStr	ProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization
title_full_unstemmed	ProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization
title_sort	probedealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/31111c7780434b0eb86d2f873fb1a7f4
work_keys_str_mv	AT mengweihu probedealerisaconvenienttoolfordesigningprobesforhighlymultiplexedfluorescenceinsituhybridization AT bingyang probedealerisaconvenienttoolfordesigningprobesforhighlymultiplexedfluorescenceinsituhybridization AT yubaocheng probedealerisaconvenienttoolfordesigningprobesforhighlymultiplexedfluorescenceinsituhybridization AT jonathansdradda probedealerisaconvenienttoolfordesigningprobesforhighlymultiplexedfluorescenceinsituhybridization AT yanbochen probedealerisaconvenienttoolfordesigningprobesforhighlymultiplexedfluorescenceinsituhybridization AT miaoliu probedealerisaconvenienttoolfordesigningprobesforhighlymultiplexedfluorescenceinsituhybridization AT siyuanwang probedealerisaconvenienttoolfordesigningprobesforhighlymultiplexedfluorescenceinsituhybridization
_version_	1718392785647697920

ProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization

Ejemplares similares