In vitro blood cell viability profiling of polymers used in molecular assembly

Abstract Biocompatible polymers have been extensively applied to molecular assembly techniques on a micro- and nanoscale to miniaturize functional devices for biomedical uses. However, cytotoxic assessments of developed devices are prone to partially focus on non-specific cells or cells associated w...

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Autores principales:	Hyejoong Jeong, Jangsun Hwang, Hwankyu Lee, Paula T. Hammond, Jonghoon Choi, Jinkee Hong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3113a523c04f4939a844dc8f2dabf8d9
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spelling	oai:doaj.org-article:3113a523c04f4939a844dc8f2dabf8d92021-12-02T15:05:34ZIn vitro blood cell viability profiling of polymers used in molecular assembly10.1038/s41598-017-10169-52045-2322https://doaj.org/article/3113a523c04f4939a844dc8f2dabf8d92017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-10169-5https://doaj.org/toc/2045-2322Abstract Biocompatible polymers have been extensively applied to molecular assembly techniques on a micro- and nanoscale to miniaturize functional devices for biomedical uses. However, cytotoxic assessments of developed devices are prone to partially focus on non-specific cells or cells associated with the specific applications. Thereby, since toxicity is dependent on the type of cells and protocols, we do not fully understand the relative toxicities of polymers. Additionally, we need to ensure the blood cell biocompatibility of developed devices prior to that of targeted cells because most of the devices contact the blood before reaching the targeted regions. Motivated by this issue, we focused on screening cytotoxicity of polymers widely used for the layer-by-layer assembly technique using human blood cells. Cytotoxicity at the early stage was investigated on twenty types of polymers (positively charged, negatively charged, or neutral) and ten combination forms via hemolysis, cell viability, and AnnexinV-FITC/PI staining assays. We determined their effects on the cell membrane depending on their surface chemistry by molecular dynamics simulations. Furthermore, the toxicity of LbL-assembled nanofilms was assessed by measuring cell viability. Based on this report, researchers can produce nanofilms that are better suited for drug delivery and biomedical applications by reducing the possible cytotoxicity.Hyejoong JeongJangsun HwangHwankyu LeePaula T. HammondJonghoon ChoiJinkee HongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hyejoong Jeong Jangsun Hwang Hwankyu Lee Paula T. Hammond Jonghoon Choi Jinkee Hong In vitro blood cell viability profiling of polymers used in molecular assembly
description	Abstract Biocompatible polymers have been extensively applied to molecular assembly techniques on a micro- and nanoscale to miniaturize functional devices for biomedical uses. However, cytotoxic assessments of developed devices are prone to partially focus on non-specific cells or cells associated with the specific applications. Thereby, since toxicity is dependent on the type of cells and protocols, we do not fully understand the relative toxicities of polymers. Additionally, we need to ensure the blood cell biocompatibility of developed devices prior to that of targeted cells because most of the devices contact the blood before reaching the targeted regions. Motivated by this issue, we focused on screening cytotoxicity of polymers widely used for the layer-by-layer assembly technique using human blood cells. Cytotoxicity at the early stage was investigated on twenty types of polymers (positively charged, negatively charged, or neutral) and ten combination forms via hemolysis, cell viability, and AnnexinV-FITC/PI staining assays. We determined their effects on the cell membrane depending on their surface chemistry by molecular dynamics simulations. Furthermore, the toxicity of LbL-assembled nanofilms was assessed by measuring cell viability. Based on this report, researchers can produce nanofilms that are better suited for drug delivery and biomedical applications by reducing the possible cytotoxicity.
format	article
author	Hyejoong Jeong Jangsun Hwang Hwankyu Lee Paula T. Hammond Jonghoon Choi Jinkee Hong
author_facet	Hyejoong Jeong Jangsun Hwang Hwankyu Lee Paula T. Hammond Jonghoon Choi Jinkee Hong
author_sort	Hyejoong Jeong
title	In vitro blood cell viability profiling of polymers used in molecular assembly
title_short	In vitro blood cell viability profiling of polymers used in molecular assembly
title_full	In vitro blood cell viability profiling of polymers used in molecular assembly
title_fullStr	In vitro blood cell viability profiling of polymers used in molecular assembly
title_full_unstemmed	In vitro blood cell viability profiling of polymers used in molecular assembly
title_sort	in vitro blood cell viability profiling of polymers used in molecular assembly
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3113a523c04f4939a844dc8f2dabf8d9
work_keys_str_mv	AT hyejoongjeong invitrobloodcellviabilityprofilingofpolymersusedinmolecularassembly AT jangsunhwang invitrobloodcellviabilityprofilingofpolymersusedinmolecularassembly AT hwankyulee invitrobloodcellviabilityprofilingofpolymersusedinmolecularassembly AT paulathammond invitrobloodcellviabilityprofilingofpolymersusedinmolecularassembly AT jonghoonchoi invitrobloodcellviabilityprofilingofpolymersusedinmolecularassembly AT jinkeehong invitrobloodcellviabilityprofilingofpolymersusedinmolecularassembly
_version_	1718388756882391040

In vitro blood cell viability profiling of polymers used in molecular assembly

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