Single genetic locus improvement of iron, zinc and β-carotene content in rice grains

Abstract Nearly half of the world’s population obtains its daily calories from rice grains, which lack or have insufficient levels of essential micronutrients. The deficiency of micronutrients vital for normal growth is a global health problem, and iron, zinc and vitamin A deficiencies are the most...

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Autores principales:	Simrat Pal Singh, Wilhelm Gruissem, Navreet K. Bhullar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3115eee4d7ae4d3e8e6325382e29ca12
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spelling	oai:doaj.org-article:3115eee4d7ae4d3e8e6325382e29ca122021-12-02T15:05:19ZSingle genetic locus improvement of iron, zinc and β-carotene content in rice grains10.1038/s41598-017-07198-52045-2322https://doaj.org/article/3115eee4d7ae4d3e8e6325382e29ca122017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07198-5https://doaj.org/toc/2045-2322Abstract Nearly half of the world’s population obtains its daily calories from rice grains, which lack or have insufficient levels of essential micronutrients. The deficiency of micronutrients vital for normal growth is a global health problem, and iron, zinc and vitamin A deficiencies are the most prevalent ones. We developed rice lines expressing Arabidopsis NICOTIANAMINE SYNTHASE 1 (AtNAS1), bean FERRITIN (PvFERRITIN), bacterial CAROTENE DESATURASE (CRTI) and maize PHYTOENE SYNTHASE (ZmPSY) in a single genetic locus in order to increase iron, zinc and β-carotene content in the rice endosperm. NAS catalyzes the synthesis of nicotianamine (NA), which is a precursor of deoxymugeneic acid (DMA) iron and zinc chelators, and also chelate iron and zinc for long distance transport. FERRITIN provides efficient storage of up to 4500 iron ions. PSY catalyzes the conversion of GGDP to phytoene, and CRTI performs the function of desaturases required for the synthesis of β-carotene from phytoene. All transgenic rice lines have significantly increased β-carotene, iron, and zinc content in the polished rice grains. Our results establish a proof-of-concept for multi-nutrient enrichment of rice grains from a single genetic locus, thus offering a sustainable and effective approach to address different micronutrient deficiencies at once.Simrat Pal SinghWilhelm GruissemNavreet K. BhullarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Simrat Pal Singh Wilhelm Gruissem Navreet K. Bhullar Single genetic locus improvement of iron, zinc and β-carotene content in rice grains
description	Abstract Nearly half of the world’s population obtains its daily calories from rice grains, which lack or have insufficient levels of essential micronutrients. The deficiency of micronutrients vital for normal growth is a global health problem, and iron, zinc and vitamin A deficiencies are the most prevalent ones. We developed rice lines expressing Arabidopsis NICOTIANAMINE SYNTHASE 1 (AtNAS1), bean FERRITIN (PvFERRITIN), bacterial CAROTENE DESATURASE (CRTI) and maize PHYTOENE SYNTHASE (ZmPSY) in a single genetic locus in order to increase iron, zinc and β-carotene content in the rice endosperm. NAS catalyzes the synthesis of nicotianamine (NA), which is a precursor of deoxymugeneic acid (DMA) iron and zinc chelators, and also chelate iron and zinc for long distance transport. FERRITIN provides efficient storage of up to 4500 iron ions. PSY catalyzes the conversion of GGDP to phytoene, and CRTI performs the function of desaturases required for the synthesis of β-carotene from phytoene. All transgenic rice lines have significantly increased β-carotene, iron, and zinc content in the polished rice grains. Our results establish a proof-of-concept for multi-nutrient enrichment of rice grains from a single genetic locus, thus offering a sustainable and effective approach to address different micronutrient deficiencies at once.
format	article
author	Simrat Pal Singh Wilhelm Gruissem Navreet K. Bhullar
author_facet	Simrat Pal Singh Wilhelm Gruissem Navreet K. Bhullar
author_sort	Simrat Pal Singh
title	Single genetic locus improvement of iron, zinc and β-carotene content in rice grains
title_short	Single genetic locus improvement of iron, zinc and β-carotene content in rice grains
title_full	Single genetic locus improvement of iron, zinc and β-carotene content in rice grains
title_fullStr	Single genetic locus improvement of iron, zinc and β-carotene content in rice grains
title_full_unstemmed	Single genetic locus improvement of iron, zinc and β-carotene content in rice grains
title_sort	single genetic locus improvement of iron, zinc and β-carotene content in rice grains
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/3115eee4d7ae4d3e8e6325382e29ca12
work_keys_str_mv	AT simratpalsingh singlegeneticlocusimprovementofironzincandbcarotenecontentinricegrains AT wilhelmgruissem singlegeneticlocusimprovementofironzincandbcarotenecontentinricegrains AT navreetkbhullar singlegeneticlocusimprovementofironzincandbcarotenecontentinricegrains
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Single genetic locus improvement of iron, zinc and β-carotene content in rice grains

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