Identification of the <i>Capsicum baccatum</i> NLR Protein CbAR9 Conferring Disease Resistance to Anthracnose
Anthracnose is caused by <i>Colletotrichum</i> species and is one of the most virulent fungal diseases affecting chili pepper (<i>Capsicum</i>) yield globally. However, the noble genes conferring resistance to <i>Colletotrichum</i> species remain largely elusive....
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| Autores principales: | , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/311af52c9a2243079b61c413bcddf4de |
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| Sumario: | Anthracnose is caused by <i>Colletotrichum</i> species and is one of the most virulent fungal diseases affecting chili pepper (<i>Capsicum</i>) yield globally. However, the noble genes conferring resistance to <i>Colletotrichum</i> species remain largely elusive. In this study, we identified <i>CbAR9</i> as the causal locus underlying the large effect quantitative trait locus <i>CcR9</i> from the anthracnose-resistant chili pepper variety PBC80. <i>CbAR9</i> encodes a nucleotide-binding and leucine-rich repeat (NLR) protein related to defense-associated NLRs in several other plant species. <i>CbAR9</i> transcript levels were induced dramatically after <i>Colletotrichum capsici</i> infection. To explore the biological function, we generated transgenic <i>Nicotiana benthamiana</i> lines overexpressing <i>CbAR9</i>, which showed enhanced resistance to <i>C. capsici</i> relative to wild-type plants. Transcript levels of pathogenesis-related (<i>PR</i>) genes increased markedly in <i>CbAR9</i>-overexpressing <i>N. benthamiana</i> plants. Moreover, resistance to anthracnose and transcript levels of <i>PR1</i> and <i>PR2</i> were markedly reduced in <i>CbAR9</i>-silenced chili pepper fruits after <i>C. capsici</i> infection. Our results revealed that CbAR9 contributes to innate immunity against <i>C. capsici</i>. |
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