Identification of the Capsicum baccatum NLR Protein CbAR9 Conferring Disease Resistance to Anthracnose

Anthracnose is caused by Colletotrichum species and is one of the most virulent fungal diseases affecting chili pepper (Capsicum) yield globally. However, the noble genes conferring resistance to Colletotrichum species remain largely elusive....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Seungmin Son, Soohong Kim, Kyong Sil Lee, Jun Oh, Inchan Choi, Jae Wahng Do, Jae Bok Yoon, Jungheon Han, Doil Choi, Sang Ryeol Park
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Capsicum baccatum</i> <i>Colletotrichum capsici</i> innate immunity <i>Nicotiana benthamiana</i> nucleotide-binding and leucine-rich repeat Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/311af52c9a2243079b61c413bcddf4de
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:311af52c9a2243079b61c413bcddf4de
record_format	dspace
spelling	oai:doaj.org-article:311af52c9a2243079b61c413bcddf4de2021-11-25T17:58:19ZIdentification of the <i>Capsicum baccatum</i> NLR Protein CbAR9 Conferring Disease Resistance to Anthracnose10.3390/ijms2222126121422-00671661-6596https://doaj.org/article/311af52c9a2243079b61c413bcddf4de2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12612https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Anthracnose is caused by <i>Colletotrichum</i> species and is one of the most virulent fungal diseases affecting chili pepper (<i>Capsicum</i>) yield globally. However, the noble genes conferring resistance to <i>Colletotrichum</i> species remain largely elusive. In this study, we identified <i>CbAR9</i> as the causal locus underlying the large effect quantitative trait locus <i>CcR9</i> from the anthracnose-resistant chili pepper variety PBC80. <i>CbAR9</i> encodes a nucleotide-binding and leucine-rich repeat (NLR) protein related to defense-associated NLRs in several other plant species. <i>CbAR9</i> transcript levels were induced dramatically after <i>Colletotrichum capsici</i> infection. To explore the biological function, we generated transgenic <i>Nicotiana benthamiana</i> lines overexpressing <i>CbAR9</i>, which showed enhanced resistance to <i>C. capsici</i> relative to wild-type plants. Transcript levels of pathogenesis-related (<i>PR</i>) genes increased markedly in <i>CbAR9</i>-overexpressing <i>N. benthamiana</i> plants. Moreover, resistance to anthracnose and transcript levels of <i>PR1</i> and <i>PR2</i> were markedly reduced in <i>CbAR9</i>-silenced chili pepper fruits after <i>C. capsici</i> infection. Our results revealed that CbAR9 contributes to innate immunity against <i>C. capsici</i>.Seungmin SonSoohong KimKyong Sil LeeJun OhInchan ChoiJae Wahng DoJae Bok YoonJungheon HanDoil ChoiSang Ryeol ParkMDPI AGarticle<i>Capsicum baccatum</i><i>Colletotrichum capsici</i>innate immunity<i>Nicotiana benthamiana</i>nucleotide-binding and leucine-rich repeatBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12612, p 12612 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Capsicum baccatum</i> <i>Colletotrichum capsici</i> innate immunity <i>Nicotiana benthamiana</i> nucleotide-binding and leucine-rich repeat Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Capsicum baccatum</i> <i>Colletotrichum capsici</i> innate immunity <i>Nicotiana benthamiana</i> nucleotide-binding and leucine-rich repeat Biology (General) QH301-705.5 Chemistry QD1-999 Seungmin Son Soohong Kim Kyong Sil Lee Jun Oh Inchan Choi Jae Wahng Do Jae Bok Yoon Jungheon Han Doil Choi Sang Ryeol Park Identification of the <i>Capsicum baccatum</i> NLR Protein CbAR9 Conferring Disease Resistance to Anthracnose
description	Anthracnose is caused by <i>Colletotrichum</i> species and is one of the most virulent fungal diseases affecting chili pepper (<i>Capsicum</i>) yield globally. However, the noble genes conferring resistance to <i>Colletotrichum</i> species remain largely elusive. In this study, we identified <i>CbAR9</i> as the causal locus underlying the large effect quantitative trait locus <i>CcR9</i> from the anthracnose-resistant chili pepper variety PBC80. <i>CbAR9</i> encodes a nucleotide-binding and leucine-rich repeat (NLR) protein related to defense-associated NLRs in several other plant species. <i>CbAR9</i> transcript levels were induced dramatically after <i>Colletotrichum capsici</i> infection. To explore the biological function, we generated transgenic <i>Nicotiana benthamiana</i> lines overexpressing <i>CbAR9</i>, which showed enhanced resistance to <i>C. capsici</i> relative to wild-type plants. Transcript levels of pathogenesis-related (<i>PR</i>) genes increased markedly in <i>CbAR9</i>-overexpressing <i>N. benthamiana</i> plants. Moreover, resistance to anthracnose and transcript levels of <i>PR1</i> and <i>PR2</i> were markedly reduced in <i>CbAR9</i>-silenced chili pepper fruits after <i>C. capsici</i> infection. Our results revealed that CbAR9 contributes to innate immunity against <i>C. capsici</i>.
format	article
author	Seungmin Son Soohong Kim Kyong Sil Lee Jun Oh Inchan Choi Jae Wahng Do Jae Bok Yoon Jungheon Han Doil Choi Sang Ryeol Park
author_facet	Seungmin Son Soohong Kim Kyong Sil Lee Jun Oh Inchan Choi Jae Wahng Do Jae Bok Yoon Jungheon Han Doil Choi Sang Ryeol Park
author_sort	Seungmin Son
title	Identification of the <i>Capsicum baccatum</i> NLR Protein CbAR9 Conferring Disease Resistance to Anthracnose
title_short	Identification of the <i>Capsicum baccatum</i> NLR Protein CbAR9 Conferring Disease Resistance to Anthracnose
title_full	Identification of the <i>Capsicum baccatum</i> NLR Protein CbAR9 Conferring Disease Resistance to Anthracnose
title_fullStr	Identification of the <i>Capsicum baccatum</i> NLR Protein CbAR9 Conferring Disease Resistance to Anthracnose
title_full_unstemmed	Identification of the <i>Capsicum baccatum</i> NLR Protein CbAR9 Conferring Disease Resistance to Anthracnose
title_sort	identification of the <i>capsicum baccatum</i> nlr protein cbar9 conferring disease resistance to anthracnose
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/311af52c9a2243079b61c413bcddf4de
work_keys_str_mv	AT seungminson identificationoftheicapsicumbaccatuminlrproteincbar9conferringdiseaseresistancetoanthracnose AT soohongkim identificationoftheicapsicumbaccatuminlrproteincbar9conferringdiseaseresistancetoanthracnose AT kyongsillee identificationoftheicapsicumbaccatuminlrproteincbar9conferringdiseaseresistancetoanthracnose AT junoh identificationoftheicapsicumbaccatuminlrproteincbar9conferringdiseaseresistancetoanthracnose AT inchanchoi identificationoftheicapsicumbaccatuminlrproteincbar9conferringdiseaseresistancetoanthracnose AT jaewahngdo identificationoftheicapsicumbaccatuminlrproteincbar9conferringdiseaseresistancetoanthracnose AT jaebokyoon identificationoftheicapsicumbaccatuminlrproteincbar9conferringdiseaseresistancetoanthracnose AT jungheonhan identificationoftheicapsicumbaccatuminlrproteincbar9conferringdiseaseresistancetoanthracnose AT doilchoi identificationoftheicapsicumbaccatuminlrproteincbar9conferringdiseaseresistancetoanthracnose AT sangryeolpark identificationoftheicapsicumbaccatuminlrproteincbar9conferringdiseaseresistancetoanthracnose
_version_	1718411792775905280

Identification of the <i>Capsicum baccatum</i> NLR Protein CbAR9 Conferring Disease Resistance to Anthracnose

Ejemplares similares