Identification and Characterization of Circular Single-Stranded DNA Genomes in Sheep and Goat Milk

In recent years, a variety of circular replicase-encoding single-stranded (CRESS) DNA viruses and unclassified virus-like DNA elements have been discovered in a broad range of animal species and environmental samples. Key questions to be answered concern their presence in the human diet and their po...

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Autores principales:	Marie-Thérèse König, Robert Fux, Ellen Link, Gerd Sutter, Erwin Märtlbauer, Andrea Didier
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	BMMF circular ssDNA colon/breast cancer <i>Cressdnaviricota</i> <i>Genomoviridae</i> milk Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/311ce4235926457aa0fda14b7b0bddd8
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spelling	oai:doaj.org-article:311ce4235926457aa0fda14b7b0bddd82021-11-25T19:13:05ZIdentification and Characterization of Circular Single-Stranded DNA Genomes in Sheep and Goat Milk10.3390/v131121761999-4915https://doaj.org/article/311ce4235926457aa0fda14b7b0bddd82021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2176https://doaj.org/toc/1999-4915In recent years, a variety of circular replicase-encoding single-stranded (CRESS) DNA viruses and unclassified virus-like DNA elements have been discovered in a broad range of animal species and environmental samples. Key questions to be answered concern their presence in the human diet and their potential impact on disease emergence. Especially DNA elements termed bovine meat and milk factors (BMMF) are suspected to act as co-factors in the development of colon and breast cancer. To expand our knowledge on the occurrence of these potential pathogens in human nutrition, a total of 73 sheep and 40 goat milk samples were assayed by combining rolling circle amplification (RCA), PCR and Sanger sequencing. The present study further includes retail milk from the aforementioned species. We recovered 15 single stranded (ss) circular genomes. Of those, nine belong to the family <i>Genomoviridae</i> and six are members of the unclassified group of BMMF. Thus, dairy sheep and goats add to dispersal of CRESS viruses and circular ssDNA elements, which enter the food chain via milk. The presence of these entities is therefore more widespread in <i>Bovidae</i> than initially assumed and seems to be part of the common human nutrition.Marie-Thérèse KönigRobert FuxEllen LinkGerd SutterErwin MärtlbauerAndrea DidierMDPI AGarticleBMMFcircular ssDNAcolon/breast cancer<i>Cressdnaviricota</i><i>Genomoviridae</i>milkMicrobiologyQR1-502ENViruses, Vol 13, Iss 2176, p 2176 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	BMMF circular ssDNA colon/breast cancer <i>Cressdnaviricota</i> <i>Genomoviridae</i> milk Microbiology QR1-502
spellingShingle	BMMF circular ssDNA colon/breast cancer <i>Cressdnaviricota</i> <i>Genomoviridae</i> milk Microbiology QR1-502 Marie-Thérèse König Robert Fux Ellen Link Gerd Sutter Erwin Märtlbauer Andrea Didier Identification and Characterization of Circular Single-Stranded DNA Genomes in Sheep and Goat Milk
description	In recent years, a variety of circular replicase-encoding single-stranded (CRESS) DNA viruses and unclassified virus-like DNA elements have been discovered in a broad range of animal species and environmental samples. Key questions to be answered concern their presence in the human diet and their potential impact on disease emergence. Especially DNA elements termed bovine meat and milk factors (BMMF) are suspected to act as co-factors in the development of colon and breast cancer. To expand our knowledge on the occurrence of these potential pathogens in human nutrition, a total of 73 sheep and 40 goat milk samples were assayed by combining rolling circle amplification (RCA), PCR and Sanger sequencing. The present study further includes retail milk from the aforementioned species. We recovered 15 single stranded (ss) circular genomes. Of those, nine belong to the family <i>Genomoviridae</i> and six are members of the unclassified group of BMMF. Thus, dairy sheep and goats add to dispersal of CRESS viruses and circular ssDNA elements, which enter the food chain via milk. The presence of these entities is therefore more widespread in <i>Bovidae</i> than initially assumed and seems to be part of the common human nutrition.
format	article
author	Marie-Thérèse König Robert Fux Ellen Link Gerd Sutter Erwin Märtlbauer Andrea Didier
author_facet	Marie-Thérèse König Robert Fux Ellen Link Gerd Sutter Erwin Märtlbauer Andrea Didier
author_sort	Marie-Thérèse König
title	Identification and Characterization of Circular Single-Stranded DNA Genomes in Sheep and Goat Milk
title_short	Identification and Characterization of Circular Single-Stranded DNA Genomes in Sheep and Goat Milk
title_full	Identification and Characterization of Circular Single-Stranded DNA Genomes in Sheep and Goat Milk
title_fullStr	Identification and Characterization of Circular Single-Stranded DNA Genomes in Sheep and Goat Milk
title_full_unstemmed	Identification and Characterization of Circular Single-Stranded DNA Genomes in Sheep and Goat Milk
title_sort	identification and characterization of circular single-stranded dna genomes in sheep and goat milk
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/311ce4235926457aa0fda14b7b0bddd8
work_keys_str_mv	AT marietheresekonig identificationandcharacterizationofcircularsinglestrandeddnagenomesinsheepandgoatmilk AT robertfux identificationandcharacterizationofcircularsinglestrandeddnagenomesinsheepandgoatmilk AT ellenlink identificationandcharacterizationofcircularsinglestrandeddnagenomesinsheepandgoatmilk AT gerdsutter identificationandcharacterizationofcircularsinglestrandeddnagenomesinsheepandgoatmilk AT erwinmartlbauer identificationandcharacterizationofcircularsinglestrandeddnagenomesinsheepandgoatmilk AT andreadidier identificationandcharacterizationofcircularsinglestrandeddnagenomesinsheepandgoatmilk
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Identification and Characterization of Circular Single-Stranded DNA Genomes in Sheep and Goat Milk

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