Molecular and Serological Techniques to Determine the Acute and Chronic Phase of Toxoplasmosis in HIV Patients

BACKGROUND AND OBJECTIVE: Toxoplasma gondii is associated with several complications including neurological problems, ocular damage and encephalitis in immunodeficiency individuals. Early diagnosis of this infection can lead to better management of this disease. Therefore, this study was conducted t...

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Autores principales:	F Shokri, R Abbaszadeh, M Rrostamnezhad, S Heydari digeh sara, N Marandi, A Bairami kuzeh konan, M Abolhasani, MJ Gharavi
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2019
Materias:	hiv toxoplasma gondii serological and molecular methods Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/311d759a91ba47aabfffc2a12e04a8f2
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spelling	oai:doaj.org-article:311d759a91ba47aabfffc2a12e04a8f22021-11-10T08:30:36ZMolecular and Serological Techniques to Determine the Acute and Chronic Phase of Toxoplasmosis in HIV Patients1561-41072251-7170https://doaj.org/article/311d759a91ba47aabfffc2a12e04a8f22019-12-01T00:00:00Zhttp://jbums.org/article-1-7987-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Toxoplasma gondii is associated with several complications including neurological problems, ocular damage and encephalitis in immunodeficiency individuals. Early diagnosis of this infection can lead to better management of this disease. Therefore, this study was conducted to determine the presence of Toxoplasma gondii with two serologic and molecular methods in HIV-infected individuals. METHODS: In this cross-sectional study, 102 male patients with HIV with a mean age of 40 ± 9.2 years were examined. The serum sample was used for ELISA to determine the acute and chronic phase and cellular samples using Real Time-PCR for determining the acute phase of the disease. The relationship between age groups and the HIV transmission pathway, as well as the age group, was compared with the results of the Toxoplasma gondii test. FINDINGS: Out of 102 samples tested for IgM anti-Toxoplasma gondii antigen by ELFA, all (100%) samples were negative, but for anti-IgG anti-parasite, 44 samples (43.1%) were positive and 58 Sample (56.9%) was negative. Out of 102 samples tested by RT-PCR, all (100%) samples were negative for Toxoplasma DNA. There was a statistically significant relationship between age groups and transmission pathways (p<0.001), as well as between age groups with anti-Toxoplasma gondii IgG levels (p<0.001). CONCLUSION: According to the results of this study, the use of IgM-ELFA and PCR-RT methods for the diagnosis of acute phase and IgG-ELFA in the chronic phase of the disease is important. With the diagnosis of chronic form of toxoplasmosis, preventive treatments can be used in HIV + patients.F ShokriR AbbaszadehM RrostamnezhadS Heydari digeh saraN MarandiA Bairami kuzeh konanM AbolhasaniMJ GharaviBabol University of Medical Sciencesarticlehivtoxoplasma gondiiserological and molecular methodsMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 21, Iss 1, Pp 140-146 (2019)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	hiv toxoplasma gondii serological and molecular methods Medicine R Medicine (General) R5-920
spellingShingle	hiv toxoplasma gondii serological and molecular methods Medicine R Medicine (General) R5-920 F Shokri R Abbaszadeh M Rrostamnezhad S Heydari digeh sara N Marandi A Bairami kuzeh konan M Abolhasani MJ Gharavi Molecular and Serological Techniques to Determine the Acute and Chronic Phase of Toxoplasmosis in HIV Patients
description	BACKGROUND AND OBJECTIVE: Toxoplasma gondii is associated with several complications including neurological problems, ocular damage and encephalitis in immunodeficiency individuals. Early diagnosis of this infection can lead to better management of this disease. Therefore, this study was conducted to determine the presence of Toxoplasma gondii with two serologic and molecular methods in HIV-infected individuals. METHODS: In this cross-sectional study, 102 male patients with HIV with a mean age of 40 ± 9.2 years were examined. The serum sample was used for ELISA to determine the acute and chronic phase and cellular samples using Real Time-PCR for determining the acute phase of the disease. The relationship between age groups and the HIV transmission pathway, as well as the age group, was compared with the results of the Toxoplasma gondii test. FINDINGS: Out of 102 samples tested for IgM anti-Toxoplasma gondii antigen by ELFA, all (100%) samples were negative, but for anti-IgG anti-parasite, 44 samples (43.1%) were positive and 58 Sample (56.9%) was negative. Out of 102 samples tested by RT-PCR, all (100%) samples were negative for Toxoplasma DNA. There was a statistically significant relationship between age groups and transmission pathways (p<0.001), as well as between age groups with anti-Toxoplasma gondii IgG levels (p<0.001). CONCLUSION: According to the results of this study, the use of IgM-ELFA and PCR-RT methods for the diagnosis of acute phase and IgG-ELFA in the chronic phase of the disease is important. With the diagnosis of chronic form of toxoplasmosis, preventive treatments can be used in HIV + patients.
format	article
author	F Shokri R Abbaszadeh M Rrostamnezhad S Heydari digeh sara N Marandi A Bairami kuzeh konan M Abolhasani MJ Gharavi
author_facet	F Shokri R Abbaszadeh M Rrostamnezhad S Heydari digeh sara N Marandi A Bairami kuzeh konan M Abolhasani MJ Gharavi
author_sort	F Shokri
title	Molecular and Serological Techniques to Determine the Acute and Chronic Phase of Toxoplasmosis in HIV Patients
title_short	Molecular and Serological Techniques to Determine the Acute and Chronic Phase of Toxoplasmosis in HIV Patients
title_full	Molecular and Serological Techniques to Determine the Acute and Chronic Phase of Toxoplasmosis in HIV Patients
title_fullStr	Molecular and Serological Techniques to Determine the Acute and Chronic Phase of Toxoplasmosis in HIV Patients
title_full_unstemmed	Molecular and Serological Techniques to Determine the Acute and Chronic Phase of Toxoplasmosis in HIV Patients
title_sort	molecular and serological techniques to determine the acute and chronic phase of toxoplasmosis in hiv patients
publisher	Babol University of Medical Sciences
publishDate	2019
url	https://doaj.org/article/311d759a91ba47aabfffc2a12e04a8f2
work_keys_str_mv	AT fshokri molecularandserologicaltechniquestodeterminetheacuteandchronicphaseoftoxoplasmosisinhivpatients AT rabbaszadeh molecularandserologicaltechniquestodeterminetheacuteandchronicphaseoftoxoplasmosisinhivpatients AT mrrostamnezhad molecularandserologicaltechniquestodeterminetheacuteandchronicphaseoftoxoplasmosisinhivpatients AT sheydaridigehsara molecularandserologicaltechniquestodeterminetheacuteandchronicphaseoftoxoplasmosisinhivpatients AT nmarandi molecularandserologicaltechniquestodeterminetheacuteandchronicphaseoftoxoplasmosisinhivpatients AT abairamikuzehkonan molecularandserologicaltechniquestodeterminetheacuteandchronicphaseoftoxoplasmosisinhivpatients AT mabolhasani molecularandserologicaltechniquestodeterminetheacuteandchronicphaseoftoxoplasmosisinhivpatients AT mjgharavi molecularandserologicaltechniquestodeterminetheacuteandchronicphaseoftoxoplasmosisinhivpatients
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Molecular and Serological Techniques to Determine the Acute and Chronic Phase of Toxoplasmosis in HIV Patients

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