Symbiotic Adaptation Drives Genome Streamlining of the Cyanobacterial Sponge Symbiont “<italic toggle="yes">Candidatus</italic> Synechococcus spongiarum”

ABSTRACT “Candidatus Synechococcus spongiarum” is a cyanobacterial symbiont widely distributed in sponges, but its functions at the genome level remain unknown. Here, we obtained the draft genome (1.66 Mbp, 90% estimated genome recovery) of “Ca. Synechococcus spongiarum” strain SH4 inhabiting the Re...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhao-Ming Gao, Yong Wang, Ren-Mao Tian, Yue Him Wong, Zenon B. Batang, Abdulaz M. Al-Suwailem, Vladimir B. Bajic, Pei-Yuan Qian
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/312274bd17584193952d608d70e9ca0d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:312274bd17584193952d608d70e9ca0d
record_format	dspace
spelling	oai:doaj.org-article:312274bd17584193952d608d70e9ca0d2021-11-15T15:45:13ZSymbiotic Adaptation Drives Genome Streamlining of the Cyanobacterial Sponge Symbiont “<italic toggle="yes">Candidatus</italic> Synechococcus spongiarum”10.1128/mBio.00079-142150-7511https://doaj.org/article/312274bd17584193952d608d70e9ca0d2014-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00079-14https://doaj.org/toc/2150-7511ABSTRACT “Candidatus Synechococcus spongiarum” is a cyanobacterial symbiont widely distributed in sponges, but its functions at the genome level remain unknown. Here, we obtained the draft genome (1.66 Mbp, 90% estimated genome recovery) of “Ca. Synechococcus spongiarum” strain SH4 inhabiting the Red Sea sponge Carteriospongia foliascens. Phylogenomic analysis revealed a high dissimilarity between SH4 and free-living cyanobacterial strains. Essential functions, such as photosynthesis, the citric acid cycle, and DNA replication, were detected in SH4. Eukaryoticlike domains that play important roles in sponge-symbiont interactions were identified exclusively in the symbiont. However, SH4 could not biosynthesize methionine and polyamines and had lost partial genes encoding low-molecular-weight peptides of the photosynthesis complex, antioxidant enzymes, DNA repair enzymes, and proteins involved in resistance to environmental toxins and in biosynthesis of capsular and extracellular polysaccharides. These genetic modifications imply that “Ca. Synechococcus spongiarum” SH4 represents a low-light-adapted cyanobacterial symbiont and has undergone genome streamlining to adapt to the sponge’s mild intercellular environment. IMPORTANCE Although the diversity of sponge-associated microbes has been widely studied, genome-level research on sponge symbionts and their symbiotic mechanisms is rare because they are unculturable. “Candidatus Synechococcus spongiarum” is a widely distributed uncultivated cyanobacterial sponge symbiont. The genome of this symbiont will help to characterize its evolutionary relationship and functional dissimilarity to closely related free-living cyanobacterial strains. Knowledge of its adaptive mechanism to the sponge host also depends on the genome-level research. The data presented here provided an alternative strategy to obtain the draft genome of “Ca. Synechococcus spongiarum” strain SH4 and provide insight into its evolutionary and functional features.Zhao-Ming GaoYong WangRen-Mao TianYue Him WongZenon B. BatangAbdulaz M. Al-SuwailemVladimir B. BajicPei-Yuan QianAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 2 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Zhao-Ming Gao Yong Wang Ren-Mao Tian Yue Him Wong Zenon B. Batang Abdulaz M. Al-Suwailem Vladimir B. Bajic Pei-Yuan Qian Symbiotic Adaptation Drives Genome Streamlining of the Cyanobacterial Sponge Symbiont “<italic toggle="yes">Candidatus</italic> Synechococcus spongiarum”
description	ABSTRACT “Candidatus Synechococcus spongiarum” is a cyanobacterial symbiont widely distributed in sponges, but its functions at the genome level remain unknown. Here, we obtained the draft genome (1.66 Mbp, 90% estimated genome recovery) of “Ca. Synechococcus spongiarum” strain SH4 inhabiting the Red Sea sponge Carteriospongia foliascens. Phylogenomic analysis revealed a high dissimilarity between SH4 and free-living cyanobacterial strains. Essential functions, such as photosynthesis, the citric acid cycle, and DNA replication, were detected in SH4. Eukaryoticlike domains that play important roles in sponge-symbiont interactions were identified exclusively in the symbiont. However, SH4 could not biosynthesize methionine and polyamines and had lost partial genes encoding low-molecular-weight peptides of the photosynthesis complex, antioxidant enzymes, DNA repair enzymes, and proteins involved in resistance to environmental toxins and in biosynthesis of capsular and extracellular polysaccharides. These genetic modifications imply that “Ca. Synechococcus spongiarum” SH4 represents a low-light-adapted cyanobacterial symbiont and has undergone genome streamlining to adapt to the sponge’s mild intercellular environment. IMPORTANCE Although the diversity of sponge-associated microbes has been widely studied, genome-level research on sponge symbionts and their symbiotic mechanisms is rare because they are unculturable. “Candidatus Synechococcus spongiarum” is a widely distributed uncultivated cyanobacterial sponge symbiont. The genome of this symbiont will help to characterize its evolutionary relationship and functional dissimilarity to closely related free-living cyanobacterial strains. Knowledge of its adaptive mechanism to the sponge host also depends on the genome-level research. The data presented here provided an alternative strategy to obtain the draft genome of “Ca. Synechococcus spongiarum” strain SH4 and provide insight into its evolutionary and functional features.
format	article
author	Zhao-Ming Gao Yong Wang Ren-Mao Tian Yue Him Wong Zenon B. Batang Abdulaz M. Al-Suwailem Vladimir B. Bajic Pei-Yuan Qian
author_facet	Zhao-Ming Gao Yong Wang Ren-Mao Tian Yue Him Wong Zenon B. Batang Abdulaz M. Al-Suwailem Vladimir B. Bajic Pei-Yuan Qian
author_sort	Zhao-Ming Gao
title	Symbiotic Adaptation Drives Genome Streamlining of the Cyanobacterial Sponge Symbiont “<italic toggle="yes">Candidatus</italic> Synechococcus spongiarum”
title_short	Symbiotic Adaptation Drives Genome Streamlining of the Cyanobacterial Sponge Symbiont “<italic toggle="yes">Candidatus</italic> Synechococcus spongiarum”
title_full	Symbiotic Adaptation Drives Genome Streamlining of the Cyanobacterial Sponge Symbiont “<italic toggle="yes">Candidatus</italic> Synechococcus spongiarum”
title_fullStr	Symbiotic Adaptation Drives Genome Streamlining of the Cyanobacterial Sponge Symbiont “<italic toggle="yes">Candidatus</italic> Synechococcus spongiarum”
title_full_unstemmed	Symbiotic Adaptation Drives Genome Streamlining of the Cyanobacterial Sponge Symbiont “<italic toggle="yes">Candidatus</italic> Synechococcus spongiarum”
title_sort	symbiotic adaptation drives genome streamlining of the cyanobacterial sponge symbiont “<italic toggle="yes">candidatus</italic> synechococcus spongiarum”
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/312274bd17584193952d608d70e9ca0d
work_keys_str_mv	AT zhaominggao symbioticadaptationdrivesgenomestreamliningofthecyanobacterialspongesymbiontitalictoggleyescandidatusitalicsynechococcusspongiarum AT yongwang symbioticadaptationdrivesgenomestreamliningofthecyanobacterialspongesymbiontitalictoggleyescandidatusitalicsynechococcusspongiarum AT renmaotian symbioticadaptationdrivesgenomestreamliningofthecyanobacterialspongesymbiontitalictoggleyescandidatusitalicsynechococcusspongiarum AT yuehimwong symbioticadaptationdrivesgenomestreamliningofthecyanobacterialspongesymbiontitalictoggleyescandidatusitalicsynechococcusspongiarum AT zenonbbatang symbioticadaptationdrivesgenomestreamliningofthecyanobacterialspongesymbiontitalictoggleyescandidatusitalicsynechococcusspongiarum AT abdulazmalsuwailem symbioticadaptationdrivesgenomestreamliningofthecyanobacterialspongesymbiontitalictoggleyescandidatusitalicsynechococcusspongiarum AT vladimirbbajic symbioticadaptationdrivesgenomestreamliningofthecyanobacterialspongesymbiontitalictoggleyescandidatusitalicsynechococcusspongiarum AT peiyuanqian symbioticadaptationdrivesgenomestreamliningofthecyanobacterialspongesymbiontitalictoggleyescandidatusitalicsynechococcusspongiarum
_version_	1718427576080269312

Symbiotic Adaptation Drives Genome Streamlining of the Cyanobacterial Sponge Symbiont “<italic toggle="yes">Candidatus</italic> Synechococcus spongiarum”

Ejemplares similares