Genetic Profiling of Sida rhombifolia Originated from Several Indonesian Ethnicities Based on Sequence-Related Amplified Polymorphism Markers

Sida rhombifolia is one of wild flowering plants that grows easily in many habitats with moderate humidity, with some usefulness in traditional medicine. Genetic characterization of Sida rhombifolia accessions originated from 12 ethnicities of Indonesia was analyzed based on Sequence-Related Amplifi...

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Autores principales:	Jumailatus Solihah, Isma Kurniatanty, Dyah Subositi, Anshary Maruzy, Ika Nugraheny Ari Martiwi, Erny Qurrotul Ainy, Khoirul Anam, Aslikh Lana Dina
Formato:	article
Lenguaje:	EN ID
Publicado:	UIN Sunan Gunung Djati 2021
Materias:	enetic characterisation, sida rhombifolia, srap Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/3123121bf1c44f63a8cdb9159445c28c
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spelling	oai:doaj.org-article:3123121bf1c44f63a8cdb9159445c28c2021-11-30T11:51:07ZGenetic Profiling of Sida rhombifolia Originated from Several Indonesian Ethnicities Based on Sequence-Related Amplified Polymorphism Markers2541-42082548-160610.15575/biodjati.v6i2.14553https://doaj.org/article/3123121bf1c44f63a8cdb9159445c28c2021-11-01T00:00:00Zhttps://journal.uinsgd.ac.id/index.php/biodjati/article/view/14553https://doaj.org/toc/2541-4208https://doaj.org/toc/2548-1606Sida rhombifolia is one of wild flowering plants that grows easily in many habitats with moderate humidity, with some usefulness in traditional medicine. Genetic characterization of Sida rhombifolia accessions originated from 12 ethnicities of Indonesia was analyzed based on Sequence-Related Amplified Polymorphism (SRAP) Markers. The genomic DNA were extracted from leaf samples and then were characterized by using the SRAP marker system according to Li and Quiros (2001). Nine pairs of SRAP primer resulted high polymorphic bands and were used in the genetic profiling. The data analysis was performed using GenAlEx to calculate genetic distance, Principal coordinate analysis, and Analysis of Molecular Variance (AMOVA), also using POPGENE to assess genetic diversity (Hs and Ht) and Nm to predict gene flow among populations. The coordinate analysis showed that the accessions originated from ethnicities along Wallacean line tend to differ genetically from most other locations. However, the results of analysis of molecular variance suggested that there were only slight differences (0.1%) found between ethnicities, while most genetic variances (99.9%) were found mostly among accessions within populations. The results suggested that there was an extensive genetic flow and plant spreading among Sida rhombifolia plant populations, resulting more homogenous genetic characters among most populations, while high diversity within population. The calculation of the number of migration (Nm = 1.7341) confirmed that the high rate of gene flow had occurred between populations.Jumailatus SolihahIsma KurniatantyDyah SubositiAnshary MaruzyIka Nugraheny Ari MartiwiErny Qurrotul AinyKhoirul AnamAslikh Lana DinaUIN Sunan Gunung Djatiarticleenetic characterisation, sida rhombifolia, srapBiology (General)QH301-705.5ENIDJurnal Biodjati, Vol 6, Iss 2, Pp 203-212 (2021)
institution	DOAJ
collection	DOAJ
language	EN ID
topic	enetic characterisation, sida rhombifolia, srap Biology (General) QH301-705.5
spellingShingle	enetic characterisation, sida rhombifolia, srap Biology (General) QH301-705.5 Jumailatus Solihah Isma Kurniatanty Dyah Subositi Anshary Maruzy Ika Nugraheny Ari Martiwi Erny Qurrotul Ainy Khoirul Anam Aslikh Lana Dina Genetic Profiling of Sida rhombifolia Originated from Several Indonesian Ethnicities Based on Sequence-Related Amplified Polymorphism Markers
description	Sida rhombifolia is one of wild flowering plants that grows easily in many habitats with moderate humidity, with some usefulness in traditional medicine. Genetic characterization of Sida rhombifolia accessions originated from 12 ethnicities of Indonesia was analyzed based on Sequence-Related Amplified Polymorphism (SRAP) Markers. The genomic DNA were extracted from leaf samples and then were characterized by using the SRAP marker system according to Li and Quiros (2001). Nine pairs of SRAP primer resulted high polymorphic bands and were used in the genetic profiling. The data analysis was performed using GenAlEx to calculate genetic distance, Principal coordinate analysis, and Analysis of Molecular Variance (AMOVA), also using POPGENE to assess genetic diversity (Hs and Ht) and Nm to predict gene flow among populations. The coordinate analysis showed that the accessions originated from ethnicities along Wallacean line tend to differ genetically from most other locations. However, the results of analysis of molecular variance suggested that there were only slight differences (0.1%) found between ethnicities, while most genetic variances (99.9%) were found mostly among accessions within populations. The results suggested that there was an extensive genetic flow and plant spreading among Sida rhombifolia plant populations, resulting more homogenous genetic characters among most populations, while high diversity within population. The calculation of the number of migration (Nm = 1.7341) confirmed that the high rate of gene flow had occurred between populations.
format	article
author	Jumailatus Solihah Isma Kurniatanty Dyah Subositi Anshary Maruzy Ika Nugraheny Ari Martiwi Erny Qurrotul Ainy Khoirul Anam Aslikh Lana Dina
author_facet	Jumailatus Solihah Isma Kurniatanty Dyah Subositi Anshary Maruzy Ika Nugraheny Ari Martiwi Erny Qurrotul Ainy Khoirul Anam Aslikh Lana Dina
author_sort	Jumailatus Solihah
title	Genetic Profiling of Sida rhombifolia Originated from Several Indonesian Ethnicities Based on Sequence-Related Amplified Polymorphism Markers
title_short	Genetic Profiling of Sida rhombifolia Originated from Several Indonesian Ethnicities Based on Sequence-Related Amplified Polymorphism Markers
title_full	Genetic Profiling of Sida rhombifolia Originated from Several Indonesian Ethnicities Based on Sequence-Related Amplified Polymorphism Markers
title_fullStr	Genetic Profiling of Sida rhombifolia Originated from Several Indonesian Ethnicities Based on Sequence-Related Amplified Polymorphism Markers
title_full_unstemmed	Genetic Profiling of Sida rhombifolia Originated from Several Indonesian Ethnicities Based on Sequence-Related Amplified Polymorphism Markers
title_sort	genetic profiling of sida rhombifolia originated from several indonesian ethnicities based on sequence-related amplified polymorphism markers
publisher	UIN Sunan Gunung Djati
publishDate	2021
url	https://doaj.org/article/3123121bf1c44f63a8cdb9159445c28c
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Genetic Profiling of Sida rhombifolia Originated from Several Indonesian Ethnicities Based on Sequence-Related Amplified Polymorphism Markers

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