Coupling chemical mutagenesis to next generation sequencing for the identification of drug resistance mutations in Leishmania

Here, Bhattacharya et al. chemically mutagenize Leishmania and identify genes associated with resistance to miltefosine and paromomycin by next generation sequencing. The study shows that a protein kinase (CDPK1) can mediate resistance to paromomycin by affecting translation.

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Autores principales:	Arijit Bhattacharya, Philippe Leprohon, Sophia Bigot, Prasad Kottayil Padmanabhan, Angana Mukherjee, Gaétan Roy, Hélène Gingras, Anais Mestdagh, Barbara Papadopoulou, Marc Ouellette
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/31297421268e443fb05fa40f1379e120
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spelling	oai:doaj.org-article:31297421268e443fb05fa40f1379e1202021-12-02T16:50:56ZCoupling chemical mutagenesis to next generation sequencing for the identification of drug resistance mutations in Leishmania10.1038/s41467-019-13344-62041-1723https://doaj.org/article/31297421268e443fb05fa40f1379e1202019-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-13344-6https://doaj.org/toc/2041-1723Here, Bhattacharya et al. chemically mutagenize Leishmania and identify genes associated with resistance to miltefosine and paromomycin by next generation sequencing. The study shows that a protein kinase (CDPK1) can mediate resistance to paromomycin by affecting translation.Arijit BhattacharyaPhilippe LeprohonSophia BigotPrasad Kottayil PadmanabhanAngana MukherjeeGaétan RoyHélène GingrasAnais MestdaghBarbara PapadopoulouMarc OuelletteNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Arijit Bhattacharya Philippe Leprohon Sophia Bigot Prasad Kottayil Padmanabhan Angana Mukherjee Gaétan Roy Hélène Gingras Anais Mestdagh Barbara Papadopoulou Marc Ouellette Coupling chemical mutagenesis to next generation sequencing for the identification of drug resistance mutations in Leishmania
description	Here, Bhattacharya et al. chemically mutagenize Leishmania and identify genes associated with resistance to miltefosine and paromomycin by next generation sequencing. The study shows that a protein kinase (CDPK1) can mediate resistance to paromomycin by affecting translation.
format	article
author	Arijit Bhattacharya Philippe Leprohon Sophia Bigot Prasad Kottayil Padmanabhan Angana Mukherjee Gaétan Roy Hélène Gingras Anais Mestdagh Barbara Papadopoulou Marc Ouellette
author_facet	Arijit Bhattacharya Philippe Leprohon Sophia Bigot Prasad Kottayil Padmanabhan Angana Mukherjee Gaétan Roy Hélène Gingras Anais Mestdagh Barbara Papadopoulou Marc Ouellette
author_sort	Arijit Bhattacharya
title	Coupling chemical mutagenesis to next generation sequencing for the identification of drug resistance mutations in Leishmania
title_short	Coupling chemical mutagenesis to next generation sequencing for the identification of drug resistance mutations in Leishmania
title_full	Coupling chemical mutagenesis to next generation sequencing for the identification of drug resistance mutations in Leishmania
title_fullStr	Coupling chemical mutagenesis to next generation sequencing for the identification of drug resistance mutations in Leishmania
title_full_unstemmed	Coupling chemical mutagenesis to next generation sequencing for the identification of drug resistance mutations in Leishmania
title_sort	coupling chemical mutagenesis to next generation sequencing for the identification of drug resistance mutations in leishmania
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/31297421268e443fb05fa40f1379e120
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Coupling chemical mutagenesis to next generation sequencing for the identification of drug resistance mutations in Leishmania

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