Applications of the indole-alkaloid gramine modulate the assembly of individual members of the barley rhizosphere microbiota

Microbial communities proliferating at the root-soil interface, collectively referred to as the rhizosphere microbiota, represent an untapped beneficial resource for plant growth, development and health. Integral to a rational manipulation of the microbiota for sustainable agriculture is the identif...

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Autores principales:	Mauro Maver, Carmen Escudero-Martinez, James Abbott, Jenny Morris, Pete E. Hedley, Tanja Mimmo, Davide Bulgarelli
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	Barley Rhizosphere Microbiota Domestication Gramine Medicine R
Acceso en línea:	https://doaj.org/article/31390aed7beb4949a5cd94a9ffffa424
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spelling	oai:doaj.org-article:31390aed7beb4949a5cd94a9ffffa4242021-11-24T15:05:12ZApplications of the indole-alkaloid gramine modulate the assembly of individual members of the barley rhizosphere microbiota10.7717/peerj.124982167-8359https://doaj.org/article/31390aed7beb4949a5cd94a9ffffa4242021-11-01T00:00:00Zhttps://peerj.com/articles/12498.pdfhttps://peerj.com/articles/12498/https://doaj.org/toc/2167-8359Microbial communities proliferating at the root-soil interface, collectively referred to as the rhizosphere microbiota, represent an untapped beneficial resource for plant growth, development and health. Integral to a rational manipulation of the microbiota for sustainable agriculture is the identification of the molecular determinants of these communities. In plants, biosynthesis of allelochemicals is centre stage in defining inter-organismal relationships in the environment. Intriguingly, this process has been moulded by domestication and breeding selection. The indole-alkaloid gramine, whose occurrence in barley (Hordeum vulgare L.) is widespread among wild genotypes but has been counter selected in several modern varieties, is a paradigmatic example of this phenomenon. This prompted us to investigate how exogenous applications of gramine impacted on the rhizosphere microbiota of two, gramine-free, elite barley varieties grown in a reference agricultural soil. High throughput 16S rRNA gene amplicon sequencing revealed that applications of gramine interfere with the proliferation of a subset of soil microbes with a relatively broad phylogenetic assignment. Strikingly, growth of these bacteria appeared to be rescued by barley plants in a genotype- and dosage-independent manner. In parallel, we discovered that host recruitment cues can interfere with the impact of gramine application in a host genotype-dependent manner. Interestingly, this latter effect displayed a bias for members of the phyla Proteobacteria. These initial observations indicate that gramine can act as a determinant of the prokaryotic communities inhabiting the root-soil interface.Mauro MaverCarmen Escudero-MartinezJames AbbottJenny MorrisPete E. HedleyTanja MimmoDavide BulgarelliPeerJ Inc.articleBarleyRhizosphereMicrobiotaDomesticationGramineMedicineRENPeerJ, Vol 9, p e12498 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Barley Rhizosphere Microbiota Domestication Gramine Medicine R
spellingShingle	Barley Rhizosphere Microbiota Domestication Gramine Medicine R Mauro Maver Carmen Escudero-Martinez James Abbott Jenny Morris Pete E. Hedley Tanja Mimmo Davide Bulgarelli Applications of the indole-alkaloid gramine modulate the assembly of individual members of the barley rhizosphere microbiota
description	Microbial communities proliferating at the root-soil interface, collectively referred to as the rhizosphere microbiota, represent an untapped beneficial resource for plant growth, development and health. Integral to a rational manipulation of the microbiota for sustainable agriculture is the identification of the molecular determinants of these communities. In plants, biosynthesis of allelochemicals is centre stage in defining inter-organismal relationships in the environment. Intriguingly, this process has been moulded by domestication and breeding selection. The indole-alkaloid gramine, whose occurrence in barley (Hordeum vulgare L.) is widespread among wild genotypes but has been counter selected in several modern varieties, is a paradigmatic example of this phenomenon. This prompted us to investigate how exogenous applications of gramine impacted on the rhizosphere microbiota of two, gramine-free, elite barley varieties grown in a reference agricultural soil. High throughput 16S rRNA gene amplicon sequencing revealed that applications of gramine interfere with the proliferation of a subset of soil microbes with a relatively broad phylogenetic assignment. Strikingly, growth of these bacteria appeared to be rescued by barley plants in a genotype- and dosage-independent manner. In parallel, we discovered that host recruitment cues can interfere with the impact of gramine application in a host genotype-dependent manner. Interestingly, this latter effect displayed a bias for members of the phyla Proteobacteria. These initial observations indicate that gramine can act as a determinant of the prokaryotic communities inhabiting the root-soil interface.
format	article
author	Mauro Maver Carmen Escudero-Martinez James Abbott Jenny Morris Pete E. Hedley Tanja Mimmo Davide Bulgarelli
author_facet	Mauro Maver Carmen Escudero-Martinez James Abbott Jenny Morris Pete E. Hedley Tanja Mimmo Davide Bulgarelli
author_sort	Mauro Maver
title	Applications of the indole-alkaloid gramine modulate the assembly of individual members of the barley rhizosphere microbiota
title_short	Applications of the indole-alkaloid gramine modulate the assembly of individual members of the barley rhizosphere microbiota
title_full	Applications of the indole-alkaloid gramine modulate the assembly of individual members of the barley rhizosphere microbiota
title_fullStr	Applications of the indole-alkaloid gramine modulate the assembly of individual members of the barley rhizosphere microbiota
title_full_unstemmed	Applications of the indole-alkaloid gramine modulate the assembly of individual members of the barley rhizosphere microbiota
title_sort	applications of the indole-alkaloid gramine modulate the assembly of individual members of the barley rhizosphere microbiota
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/31390aed7beb4949a5cd94a9ffffa424
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Applications of the indole-alkaloid gramine modulate the assembly of individual members of the barley rhizosphere microbiota

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